Identification of host proteins required for HIV infection through a functional genomic screen

Science

Volumn 319, Issue 5865, 2008, Pages 921-926

  Brass, Abraham L ^a,b   Dykxhoorn, Derek M ^c   Benita, Yair ^c   Yan, Nan ^c   Engelman, Alan ^c   Xavier, Ramnik J ^b,c   Lieberman, Judy ^c   Elledge, Stephen J ^a  

^a BRIGHAM AND WOMEN S HOSPITAL   (United States)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; CELL PROTEIN; GOLGI TRANSPORT PROTEIN RAB6; GOLGI TRANSPORT PROTEIN VPS53; KARYOPHERIN; MEDIATOR COMPLEX MED28; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

EVOLUTION; GENE EXPRESSION; GENOMICS; HOST; HUMAN IMMUNODEFICIENCY VIRUS; IDENTIFICATION METHOD; LIFE CYCLE; PROTEIN;

ARTICLE; BIOINFORMATICS; CELL FUNCTION; FUNCTIONAL GENOMICS; GENE EXPRESSION; GENETIC SCREENING; GENETIC TRANSCRIPTION; GENETICS; HIV DEPENDENCY FACTOR GENE; HOST CELL; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IMMUNOCOMPETENT CELL; LIFE CYCLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; RNA INTERFERENCE; TRANSCRIPTION REGULATION; VIRUS ENTRY; VIRUS GENE; VIRUS TRANSCRIPTION;

CELL LINE, TUMOR; COMPUTATIONAL BIOLOGY; CYTOSKELETAL PROTEINS; GENOMICS; HIV INFECTIONS; HIV-1; HOST-PATHOGEN INTERACTIONS; HUMAN IMMUNODEFICIENCY VIRUS PROTEINS; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; KARYOPHERINS; PROTEINS; RAB GTP-BINDING PROTEINS; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSCRIPTION, GENETIC; VESICULAR TRANSPORT PROTEINS; VIRUS INTEGRATION; VIRUS INTERNALIZATION; VIRUS REPLICATION;

HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 39349097864     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1152725     Document Type: Article

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37. We thank the Institute of Chemistry and Cell Biology (ICCB)-Longwood: C. Shamu, S. Rudnicki, S. Johnston, D. Fletcher, K. Schulberg, and M. Tsui. We also thank F. Diaz-Griffero, J. Walsh, A. Balazs, R. Mulligan, R. Sutton, V. Ramesh, and A. Puri for reagents and guidance; B. Glick, G. Hu, E. R. McDonald III, A. Smorgorzewska, N. Solimini, M. Krishnan, and members of the Elledge lab for help and advice; A. Liao, A. Landry, and Z. Wu for technical assistance; and E. Golde for facilitating collaborations. Funding for this study was provided by a Crohns and Colitis Foundation of America fellowship (A.L.B.), a Center for Computational and Integrative Biology fellowship (Y.B.), and the Harvard Center for AIDS Research (CFAR) scholar award (N.Y.). J.L. (U19-AI056900), R.J.X. (AI062773), and A.E. (AI052014) are supported by NIH grants. D.M.D. was funded by a grant from the Harvard CFAR to J.L. and S.J.E. S.J.E. is an Investigator with the Howard Hughes Medical Institute.