Genome-scale loss-of-function screening with a lentiviral RNAi library

Nature Methods

Volumn 3, Issue 9, 2006, Pages 715-719

  Root, David E ^a   Hacohen, Nir ^a,b,c   Hahn, William C ^a,c,d   Lander, Eric S ^a,c,e,f   Sabatini, David M ^a,e,f  

^a HARVARD UNIVERSITY   (United States)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

^d DANA FARBER CANCER INSTITUTE   (United States)

^e WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

^f MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; DOUBLE STRANDED RNA; FAS ACTIVATED SERINE THREONINE KINASE; GENOMIC DNA; LENTIVIRUS VECTOR; PHOSPHOTRANSFERASE; PLASMID DNA; PROTEIN AKT3; SHORT HAIRPIN RNA; SMALL INTERFERING RNA; UNCLASSIFIED DRUG; VIRUS DNA;

ARTICLE; BIOMEDICAL TECHNOLOGY ASSESSMENT; BIOSAFETY; CELL DEATH; CELL STRUCTURE; CORRELATION ANALYSIS; DNA MICROARRAY; FLUORESCENCE ANALYSIS; GENE AMPLIFICATION; GENE CASSETTE; GENE EXPRESSION REGULATION; GENE LIBRARY; GENE REPRESSION; GENE SILENCING; GENE TARGETING; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN GENOME; INTERMETHOD COMPARISON; LONG TERMINAL REPEAT; MAMMAL CELL; MITOSIS; MOLECULAR CLONING; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; QUALITY CONTROL; QUANTITATIVE ANALYSIS; RELIABILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA HYBRIDIZATION; RNA INTERFERENCE; RNA SEQUENCE; RNA STRUCTURE; TARGET CELL; TRANSCRIPTION REGULATION; VIRAL GENE DELIVERY SYSTEM; VIRUS GENE; VIRUS INFECTION; VIRUS REPLICATION;

ANIMALS; GENE EXPRESSION REGULATION; GENE LIBRARY; GENETIC VECTORS; HUMANS; LENTIVIRUS; MICE; RNA, SMALL INTERFERING;

LENTIVIRUS; MAMMALIA;

EID: 33747794587     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth924     Document Type: Article

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