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Characterization of a root-specific Arabidopsis terpene synthase responsible for the formation of the volatile monoterpene 1,8-cineole
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The first report of developmentally regulated expression patterns of a root terpene synthase. The expression of the gene in the epidermal cells of older root growth zones suggests direct release of the volatile monoterpene product into the rhizosphere.
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Chen F., Ro D.K., Petri J., Gershenzon J., Bohlmann J., Pichersky E., and Tholl D. Characterization of a root-specific Arabidopsis terpene synthase responsible for the formation of the volatile monoterpene 1,8-cineole. Plant Physiol 135 (2004) 1956-1966. The first report of developmentally regulated expression patterns of a root terpene synthase. The expression of the gene in the epidermal cells of older root growth zones suggests direct release of the volatile monoterpene product into the rhizosphere.
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Ro DK, Ehlting J, Keeling CI, Lin R, Mattheus N, Bohlmann J: Microarray expression profiling and functional characterization of AtTPS genes: duplicated Arabidopsis thaliana sesquiterpene synthase genes At4g13280 and At4g13300 encode root-specific and wound-inducible (Z)-γ-bisabolene synthases. Arch Biochem Biophys 2005. in press. [doi:10.1016/j.abb.2005.09.019].
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Chen F., Tholl D., D'Auria J.C., Farooq A., Pichersky E., and Gershenzon J. Biosynthesis and emission of terpenoid volatiles from Arabidopsis flowers. Plant Cell 15 (2003) 481-494
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The authors demonstrate that floral terpene volatile bouquets can be dramatically altered by silencing just two florally expressed TPS genes.
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Tholl D., Chen F., Petri J., Gershenzon J., and Pichersky E. Two sesquiterpene synthases are responsible for the complex mixture of sesquiterpenes emitted from Arabidopsis flowers. Plant J 42 (2005) 757-771. The authors demonstrate that floral terpene volatile bouquets can be dramatically altered by silencing just two florally expressed TPS genes.
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A comprehensive analysis of the evolution of isoprene synthases. The authors also report on the first successful generation of isoprene-emitting transgenic Arabidopsis.
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A fascinating example of how allelic variation and subtle differences in the primary structures of terpene synthases can change the stereoselectivity, and hence the terpene volatile profiles, of plant cultivars.
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Köllner T.G., Schnee C., Gershenzon J., and Degenhardt J. The variability of sesquiterpenes emitted from two Zea mays cultivars is controlled by allelic variation of two terpene synthase genes encoding stereoselective multiple product enzymes. Plant Cell 16 (2004) 1115-1131. A fascinating example of how allelic variation and subtle differences in the primary structures of terpene synthases can change the stereoselectivity, and hence the terpene volatile profiles, of plant cultivars.
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The authors present a detailed biochemical and molecular study showing how intraspecific variation of the expression of multiple TPS genes determines the existence of distinct chemotypes in basil. This knowledge can be exploited for breeding special chemotypes.
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Iijima Y., Davidovich-Rikanati R., Fridman E., Gang D.R., Bar E., Lewinsohn E., and Pichersky E. The biochemical and molecular basis for the divergent patterns in the biosynthesis of terpenes and phenylpropenes in the peltate glands of three cultivars of basil. Plant Physiol 136 (2004) 3724-3736. The authors present a detailed biochemical and molecular study showing how intraspecific variation of the expression of multiple TPS genes determines the existence of distinct chemotypes in basil. This knowledge can be exploited for breeding special chemotypes.
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This study represents a good example of the evolution of metabolic diversity in fruit flavors. The differential expression of two terpene synthases in cultivated and wildtype strawberry fruit has a profound effect on strawberry fruit flavor. The cytosolic localization of the predominant terpene synthase in cultivated strawberry and the formation of two aroma compounds, linalool and nerolidol, by this enzyme led the authors to suggest that both enzyme substrates (GPP and FPP) are present in the cytosol.
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Aharoni A., Giri A.P., Verstappen F.W.A., Bertea C.M., Sevenier R., Sun Z.K., Jongsma M.A., Schwab W., and Bouwmeester H.J. Gain and loss of fruit flavor compounds produced by wild and cultivated strawberry species. Plant Cell 16 (2004) 3110-3131. This study represents a good example of the evolution of metabolic diversity in fruit flavors. The differential expression of two terpene synthases in cultivated and wildtype strawberry fruit has a profound effect on strawberry fruit flavor. The cytosolic localization of the predominant terpene synthase in cultivated strawberry and the formation of two aroma compounds, linalool and nerolidol, by this enzyme led the authors to suggest that both enzyme substrates (GPP and FPP) are present in the cytosol.
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Miller B., Madilao L.L., Ralph S., and Bohlmann J. Insect-induced conifer defense. White pine weevil and methyl jasmonate induce traumatic resinosis, de novo formed volatile emissions, and accumulation of terpenoid synthase and putative octadecanoid pathway transcripts in Sitka spruce. Plant Physiol 137 (2005) 369-382
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