Metabolic engineering for advanced biofuels production from Escherichia coli

Current Opinion in Biotechnology

Volumn 19, Issue 5, 2008, Pages 414-419

  Atsumi, Shota ^a   Liao, James C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI; ETHANOL; FUELS; HYDROSTATIC PRESSURE; TECHNOLOGY; VAPOR PRESSURE;

ALTERNATIVE APPROACH; BIOFUELS PRODUCTION; ENVIRONMENTAL PROBLEMS; GLOBAL ENERGY; HIGHER ENERGY DENSITY; LIQUID BIOFUELS; LOWER VAPOR PRESSURE; METABOLIC ENGINEERING; PRODUCTION EFFICIENCIES; RECENT PROGRESS; SYNTHETIC PATHWAYS; TRANSPORTATION INFRASTRUCTURES;

BIOFUELS;

2 PROPANOL; BIOFUEL; BUTANOL; FATTY ACID ESTER; ISOBUTANOL; ISOPRENOID; OXOACID;

ALCOHOL PRODUCTION; BIOFUEL PRODUCTION; ENERGY TRANSFER; ESCHERICHIA COLI; FERMENTATION; GENETIC COMPATIBILITY; METABOLIC ENGINEERING; MICROORGANISM; NONHUMAN; PRIORITY JOURNAL; REVIEW; VAPOR PRESSURE; WETTABILITY;

ALCOHOLS; BIOELECTRIC ENERGY SOURCES; BIOTECHNOLOGY; CONSERVATION OF NATURAL RESOURCES; ESCHERICHIA COLI; FORECASTING; GASOLINE; METABOLOMICS; PROTEIN ENGINEERING;

ESCHERICHIA COLI;

EID: 53049083876     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.copbio.2008.08.008     Document Type: Review

References (27)

1. Kalscheuer R., Stolting T., and Steinbuchel A. Microdiesel: Escherichia coli engineered for fuel production. Microbiology 152 (2006) 2529-2536. This study demonstrates that ethanol can be esterified to a fatty acid in E. coli, which provides significant possibility for a whole-cell catalyst for the production of biodiesel.
2. Hanai T., Atsumi S., and Liao J.C. Engineered synthetic pathway for isopropanol production in Escherichia coli. Appl Environ Microbiol 73 (2007) 7814-7818. This paper demonstrates isopropanol production from E. coli by expressing various combinations of genes from C. acetobutylicum ATCC 824, E. coli K-12 MG1655, C. beijerinckii NRRL B593, and T. brockii HTD4. The isopropanol production from E. coli is higher than those produced by C. acetobutylicum.
3. Withers S.T., Gottlieb S.S., Lieu B., Newman J.D., and Keasling J.D. Identification of isopentenol biosynthetic genes from Bacillus subtilis by a screening method based on isoprenoid precursor toxicity. Appl Environ Microbiol 73 (2007) 6277-6283. The authors developed a screening method to isolate isoprenoid biosynthetic genes. With this method, two terpene synthase genes are cloned from the isoprene-producing bacterium Bacillus subtilis strain 6051.
4. Atsumi S., Hanai T., and Liao J.C. Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels. Nature 451 (2008) 86-89. This paper presents a metabolic engineering approach using E. coli to produce higher alcohols including isobutanol, n-butanol, 2-methyl-1-butanol, and 3-methyl-1-butanol from glucose, a renewable carbon source. This strategy uses the host's amino acid biosynthetic pathway and diverts its 2-ketoacid intermediates for alcohol synthesis.
5. Atsumi S., Cann A.F., Connor M.R., Shen C.R., Smith K.M., Brynildsen M.P., Chou K.J., Hanai T., and Liao J.C. Metabolic engineering of Escherichia coli for 1-butanol production. Metab Eng (2008) 10.1016/j.ymben.2007.08.003. Clostridium is a natural producer of n-butanol. However, the relatively unknown genetic system and complex physiology of Clostridium present difficulties in engineering its metabolism for optimal production of n-butanol. To deal with these problems, the authors engineered a synthetic pathway in E. coli and demonstrated the production of 1-butanol from this non-native user-friendly host.
6. Lin Y.L., and Blaschek H.P. Butanol production by a butanol-tolerant strain of Clostridium acetobutylicum in extruded corn broth. Appl Environ Microbiol 45 (1983) 966-973
7. Jones D.T., and Woods D.R. Acetone-butanol fermentation revisited. Microbiol Rev 50 (1986) 484-524
8. Osburn O.L., Brown R.W., and Werkman C.H. The butyl alcohol-isopropyl alcohol fermentation. J Biol Chem 121 (1937) 685-695
9. Chen J.S., and Hiu S.F. Acetone-butanol-isopropanol production by Clostridium beijerinckii (synonym, Clostridium butylicum). Biotechnol Lett 8 (1986) 371-376
10. Bermejo L.L., Welker N.E., and Papoutsakis E.T. Expression of Clostridium acetobutylicum ATCC 824 genes in Escherichia coli for acetone production and acetate detoxification. Appl Environ Microbiol 64 (1998) 1079-1085
11. Peretz M., Bogin O., Tel-Or S., Cohen A., Li G., Chen J.S., and Burstein Y. Molecular cloning, nucleotide sequencing, and expression of genes encoding alcohol dehydrogenases from the thermophile Thermoanaerobacter brockii and the mesophile Clostridium beijerinckii. Anaerobe 3 (1997) 259-270
12. Duncombe G.R., and Frerman F.E. Molecular and catalytic properties of the acetoacetyl-coenzyme A thiolase of Escherichia coli. Arch Biochem Biophys 176 (1976) 159-170
13. Jenkins L.S., and Nunn W.D. Genetic and molecular characterization of the genes involved in short-chain fatty acid degradation in Escherichia coli: the ato system. J Bacteriol 169 (1987) 42-52
14. Ismaiel A.A., Zhu C.X., Colby G.D., and Chen J.S. Purification and characterization of a primary-secondary alcohol dehydrogenase from two strains of Clostridium beijerinckii. J Bacteriol 175 (1993) 5097-5105
15. Lamed R.J., and Zeikus J.G. Novel NADP-linked alcohol-aldehyde/ketone oxidoreductase in thermophilic ethanologenic bacteria. Biochem J 195 (1981) 183-190
16. Boynton Z.L., Bennett G.N., and Rudolph F.B. Cloning, sequencing, and expression of genes encoding phosphotransacetylase and acetate kinase from Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol 62 (1996) 2758-2766
17. Fontaine L., Meynial-Salles I., Girbal L., Yang X., Croux C., and Soucaille P. Molecular characterization and transcriptional analysis of adhE2, the gene encoding the NADH-dependent aldehyde/alcohol dehydrogenase responsible for butanol production in alcohologenic cultures of Clostridium acetobutylicum ATCC 824. J Bacteriol 184 (2002) 821-830
18. Wiesenborn D.P., Rudolph F.B., and Papoutsakis E.T. Thiolase from Clostridium acetobutylicum ATCC 824 and its role in the synthesis of acids and solvents. Appl Environ Microbiol 54 (1988) 2717-2722
19. Becker D.F., Fuchs J.A., Banfield D.K., Funk W.D., MacGillivray R.T., and Stankovich M.T. Characterization of wild-type and an active-site mutant in Escherichia coli of short-chain acyl-CoA dehydrogenase from Megasphaera elsdenii. Biochemistry 32 (1993) 10736-10742
20. O'Neill H., Mayhew S.G., and Butler G. Cloning and analysis of the genes for a novel electron-transferring flavoprotein from Megasphaera elsdenii. Expression and characterization of the recombinant protein. J Biol Chem 273 (1998) 21015-21024
21. Wallace K.K., Bao Z.Y., Dai H., Digate R., Schuler G., Speedie M.K., and Reynolds K.A. Purification of crotonyl-CoA reductase from Streptomyces collinus and cloning, sequencing and expression of the corresponding gene in Escherichia coli. Eur J Biochem 233 (1995) 954-962
22. Sentheshanuganathan S. The mechanism of the formation of higher alcohols from amino acids by Saccharomyces cerevisiae. Biochem J 74 (1960) 568-576
23. de la Plaza M., Fernandez de Palencia P., Pelaez C., and Requena T. Biochemical and molecular characterization of alpha-ketoisovalerate decarboxylase, an enzyme involved in the formation of aldehydes from amino acids by Lactococcus lactis. FEMS Microbiol Lett 238 (2004) 367-374
24. Kisumi M., Sugiura M., and Chibata I. Biosynthesis of norvaline, norleucine, and homoisoleucine in Serratia marcescens. J Biochem 80 (1976) 333-339
25. Miwa K., Tsuchida T., Kurahashi O., Nakamori S., Sano K., and Momose H. Construction of l-threonine overproducing strains of Escherichia coli K-12 using recombinant. Agric Biol Chem 47 (1983) 2329-2334
26. Vaneechoutte M., Young D.M., Ornston L.N., De Baere T., Nemec A., Van Der Reijden T., Carr E., Tjernberg I., and Dijkshoorn L. Naturally transformable Acinetobacter sp. strain ADP1 belongs to the newly described species Acinetobacter baylyi. Appl Environ Microbiol 72 (2006) 932-936
27. Sacchettini J.C., and Poulter C.D. Creating isoprenoid diversity. Science 277 (1997) 1788-1789