The production of biopharmaceuticals in plant systems

Biotechnology Advances

Volumn 27, Issue 6, 2009, Pages 879-894

  Karg, Saskia R ^a   Kallio, Pauli T ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

Biopharmaceutical; Heterologous expression; Molecular farming; N glycosylation; Plants

Indexed keywords

BIOPHARMACEUTICAL; HETEROLOGOUS EXPRESSION; MOLECULAR FARMING; N-GLYCOSYLATION; PLANTS;

CELL MEMBRANES; DRUG PRODUCTS PLANTS; ESTERIFICATION; GLYCOSYLATION; INDUSTRIAL APPLICATIONS; MACHINERY; MAMMALS; PLANT CELL CULTURE; PROTEINS;

CELL CULTURE;

DRUG;

BIOCHEMICAL COMPOSITION; BIOCHEMISTRY; COST-BENEFIT ANALYSIS; GENE EXPRESSION; GENETIC ENGINEERING; MOLECULAR ANALYSIS; PHARMACEUTICAL INDUSTRY; PLANT; PROTEIN;

GLYCOSYLATION; METABOLISM; PLANT; REVIEW;

GLYCOSYLATION; PHARMACEUTICAL PREPARATIONS; PLANTS;

MAMMALIA;

EID: 70349765520     PISSN: 07349750     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biotechadv.2009.07.002     Document Type: Review

References (221)

1. Aalberse R.C., Koshte V., and Clemens J.G. Immunoglobulin E antibodies that crossreact with vegetable foods, pollen, and Hymenoptera venom. J Allergy Clin Immunol 68 (1981) 356-364
2. Abranches R., Arcalis E., Marcel S., Altmann F., Ribeiro-Pedro M., Rodriguez J., et al. Functional specialization of Medicago truncatula leaves and seeds does not affect the subcellular localization of a recombinant protein. Planta 227 (2008) 649-658
3. Altmann F. The role of protein glycosylation in allergy. Int Arch Allergy Immunol 142 (2007) 99-115
4. Arntzen C., Plotkin S., and Dodet B. Preface - plant-derived vaccines and antibodies: potential and limitations. Vaccine 23 (2005) 1753-1756
5. Artsaenko O., Kettig B., Fiedler U., Conrad U., and During K. Potato tubers as a biofactory for recombinant antibodies. Mol Breed 4 (1998) 313-319
6. Ashford D.A., Dwek R.A., Rademacher T.W., Lis H., and Sharon N. The glycosylation of glycoprotein lectins. Intra- and inter-genus variation in N-linked oligosaccharide expression. Carbohydr Res 213 (1991) 215-227
7. Ashwell G., and Harford J. Carbohydrate-specific receptors of the liver. Annu Rev Biochem 51 (1982) 531-554
8. Bailey J.E., Prati E.G.P., Jean-Mairet J., Sburlati A.R., and Umaña P. Engineering glycosylation in animal cells. In: Merten O.W. (Ed). New developments and new applications in animal cell technology (1998), Kluwer Academic Publishers
9. Baker K.N., Rendall M.H., Hills A.E., Hoare M., Freedman R.B., and James D.C. Metabolic control of recombinant protein N-glycan processing in NS0 and CHO cells. Biotechnol Bioeng 73 (2001) 188-202
10. Bakker H., Bardor M., Molthoff J.W., Gomord V., Elbers I., Stevens L.H., et al. Galactose-extended glycans of antibodies produced by transgenic plants. Proc Natl Acad Sci U S A 98 (2001) 2899-2904
11. Bakker H., Schijlen E., de Vries T., Schiphorst W., Jordi W., Lommen A., et al. Plant members of the alpha 1, 3/4-fucosyltransferase gene family encode an alpha 1, 4-fucosyltransferase, potentially involved in Lewis(a) biosynthesis, and two core alpha 1, 3-fucosyltransferases. FEBS Lett 507 (2001) 307-312
12. Bakker H., Rouwendal G.J., Karnoup A.S., Florack D.E.A., Stoopen G.M., Helsper J., et al. An antibody produced in tobacco expressing a hybrid beta-(1,4)-galactosyltransferase is essentially devoid of plant carbohydrate epitopes. Proc Natl Acad Sci U S A 103 (2006) 7577-7582
13. Balen B., and Krsnik-Rasol M. N-glycosylation of recombinant therapeutic glycoproteins in plant systems. Food Technol Biotechnol 45 (2007) 1-10
14. Bardor M., Cabrera G., Rudd P.M., Dwek R.A., Cremata J.A., and Lerouge P. Analytical strategies to investigate plant N-glycan profiles in the context of plant-made pharmaceuticals. Curr Opin Struck Biol 16 (2006) 576-583
15. Bardor M., Faveeuw C., Fitchette A.C., Gilbert D., Galas L., Trottein F., et al. Immunoreactivity in mammals of two typical plant glyco-epitopes, core alpha-(1,3)-fucose and core xylose. Glycobiology 13 (2003) 427-434
16. Bardor M., Loutelier-Bourhis C., Paccalet T., Cosette P., Fitchette A.C., Vezina L.P., et al. Monoclonal C5-1 antibody produced in transgenic alfalfa plants exhibits a N-glycosylation that is homogenous and suitable for glyco-engineering into human-compatible structures. Plant Biotechnol J 1 (2003) 451-462
17. Barta A., Sommergruber K., Thompson D., Hartmuth K., Matzke M.A., and Matzke A.J.M. The expression of a nopaline synthase - human growth-hormone chimeric gene in transformed tobacco and sunflower callus-tissue. Plant Mol Biol 6 (1986) 347-357
18. Basaran P., and Rodríguez-Cerezo E. Plant molecular farming: opportunities and challenges. Crit Rev Biotechnol 28 (2008) 153-172
19. Becerra-Arteaga A., and Shuler M.L. Influence of culture medium supplementation of tobacco NT1 cell suspension cultures on the N-glycosylation of human secreted alkaline phosphatase. Biotechnol Bioeng 97 (2007) 1585-1593
20. Benchabane M., Goulet C., Rivard D., Faye L., Gomord V., and Michaud D. Preventing unintended proteolysis in plant protein biofactories. Plant Biotechnol J 6 (2008) 633-648
21. Bencur P., Steinkellner H., Svoboda B., Mucha J., Strasser R., Kolarich D., et al. Arabidopsis thaliana beta-(1,2)-xylosyltransferase: an unusual glycosyltransferase with the potential to act at multiple stages of the plant N-glycosylation pathway. Biochem J 388 (2005) 515-525
22. Bisaria V., and Panda A. Large-scale plant cell culture: methods, applications and products. Curr Opin Biotechnol 2 (1991) 370-374
23. Blais D.R., and Altosaar I. Human CD14 expressed in seeds of transgenic tobacco displays similar proteolytic resistance and bioactivity with its mammalian-produced counterpart. Transgenic Res 15 (2006) 151-164
24. Bondili J.S., Castilho A., Mach L., Glossl J., Steinkellner H., Altmann F., et al. Molecular cloning and heterologous expression of beta-(1,2)-xylosyltransferase and core alpha-(1,3)-fucosyltransferase from maize. Phytochemistry 67 (2006) 2215-2224
25. Borisjuk N.V., Borisjuk L.G., Logendra S., Petersen F., Gleba Y., and Raskin I. Production of recombinant proteins in plant root exudates. Nat Biotechnol 17 (1999) 466-469
26. Bouquin T., Thomsen M., Nielsen L.K., Green T.H., Mundy J., and Dziegiel M.H. Human anti-rhesus D IgG1 antibody produced in transgenic plants. Transgenic Res 11 (2002) 115-122
27. Brooks S.A. Appropriate glycosylation of recombinant proteins for human use: implications of choice of expression system. Mol Biotechnol 28 (2004) 241-255
28. Brooks S.A. Protein glycosylation in diverse cell systems: implications for modification and analysis of recombinant proteins. Exp Rev Proteomics 3 (2006) 345-359
29. Butler M. Animal cell cultures: recent achievements and perspectives in the production of biopharmaceuticals. Appl Microbiol Biotechnol 68 (2005) 283-291
30. Chargelegue D., Vine N.D., van Dolleweerd C.J., Drake P.M., and Ma J.K. A murine monoclonal antibody produced in transgenic plants with plant-specific glycans is not immunogenic in mice. Transgenic Res 9 (2000) 187-194
31. Chen M., Liu X., Wang Z., Song J., Qi Q., and Wang P.G. Modification of plant N-glycans processing: the future of producing therapeutic protein by transgenic plants. Med Res Rev 25 (2005) 343-360
32. Chen P.F., and Harcum S.W. Effects of elevated ammonium on glycosylation gene expression in CHO cells. Metab Eng 8 (2006) 123-132
33. Chrispeels M.J., and Faye L. The production of recombinant glycoproteins with defined non-immunogenic glycans. In: Owen M.R.L., and Pen J. (Eds). Transgenic plants: a production system for industrial and pharmaceutical proteins (1996), John Wiley and Sons Ltd.
34. Christou P. Particle gun mediated transformation. Curr Opin Biotechnol 4 (1993) 135-141
35. Conrad U., Fiedler U., Artsaenko O., and Phillips J. High-level and stable accumulation of single-chain Fv antibodies in plant storage organs. J Plant Physiol 152 (1998) 708-711
36. Cox K.M., Sterling J.D., Regan J.T., Gasdaska J.R., Frantz K.K., Peele C.G., et al. Glycan optimization of a human monoclonal antibody in the aquatic plant Lemna minor. Nat Biotechnol 24 (2006) 1591-1597
37. Cramer C.L., Weissenborn D.L., Oishi K.K., Grabau E.A., Bennett S., Ponce E., et al. Bioproduction of human enzymes in transgenic tobacco. Ann NY Acad Sci 792 (1996) 62-71
38. Dalal M., Dani R.G., and Kumar P.A. Current trends in the genetic engineering of vegetable crops. Sci Hortic 107 (2006) 215-225
39. Daniell T., and Edwards R. Changes in protein methylation associated with the elicitation response in cell cultures of alfalfa (Medicago sativa L.). FEBS Lett 360 (1995) 57-61
40. De Wilde C., Peeters K., Jacobs A., Peck I., and Depicker A. Expression of antibodies and Fab fragments in transgenic potato plants: a case study for bulk production in crop plants. Mol Breed 9 (2002) 271-282
41. Decker E.L., and Reski R. Current achievements in the production of complex biopharmaceuticals with moss bioreactors. Bioprocess Biosyst Eng 31 (2008) 3-9
42. De Neve M., Deloose M., Jacobs A., Vanhoudt H., Kaluza B., Weidle U., Van Montagu M., and Depicker A. Assembly of an antibody and its derived antibody fragment in Nicotiana and Arabidopsis. Transgenic Res 2 (1993) 227-237
43. Dong X., Storkus W.J., and Salter R.D. Binding and uptake of a galactosyl IgG by mannose receptor on macrophages and dendritic cells. J Immunol 163 (1999) 5427-5434
44. Drake P.M.W., Chargelegue D.M., Vine N.D., van Dolleweerd C.J., Obregon P., and Ma J.K.C. Rhizosecretion of a monoclonal antibody protein complex from transgenic tobacco roots. Plant Mol Biol 52 (2003) 233-241
45. Dube D.H., and Bertozzi C.R. Glycans in cancer and inflammation-potential for therapeutics and diagnostics. Nat Rev Drug Discov 4 (2005) 477-488
46. Dus Santos M.J., Carrillo C., Ardila F., Rios R.D., Franzone P., Piccone M.E., et al. Development of transgenic alfalfa plants containing the foot and mouth disease virus structural polyprotein gene P1 and its utilization as an experimental immunogen. Vaccine 23 (2005) 1838-1843
47. Dwek R.A. Glycobiology: toward understanding the function of sugars. Chem Rev 96 (1996) 683-720
48. Faye L., Boulaflous A., Benshabane M., Gomord V., and Michaud D. Protein modifications in the plant secretory pathway: current status and practical implications in molecular pharming. Vaccine 23 (2005) 1770-1778
49. Ferrara C., Brünker P., Suter T., Moser S., Püntener U., and Umaña P. Modulation of therapeutic antibody effector functions by glycosylation engineering: Influence of Golgi enzyme localization domain and co-expression of heterologous beta-(1,4)-N-acetylglucosaminyltransferase III and Golgi alpha-mannosidase II. Biotechnol Bioeng 93 (2006) 851-861
50. Fiedler U., Phillips J., Artsaenko O., and Conrad U. Optimization of scFv antibody production in transgenic plants. Immunotechnology 3 (1997) 205-216
51. Fischer R., and Emans N. Molecular farming of pharmaceutical proteins. Transgenic Res 9 (2000) 279-299
52. Fischer R., Liao Y.C., and Drossard J. Affinity-purification of a TMV-specific recombinant full-size antibody from a transgenic tobacco suspension culture. J Immunol Methods 226 (1999) 1-10
53. Fischer R., Schumann D., Zimmermann S., Drossard J., Sack M., and Schillberg S. Expression and characterization of bispecific single-chain Fv fragments produced in transgenic plants. Eur J Biochem 262 (1999) 810-816
54. Fitchette A.C., Cabanes-Macheteau M., Marvin L., Martin B., Satiat-Jeunemaitre B., Gomord V., et al. Biosynthesis and immunolocalization of Lewis a-containing N-glycans in the plant cell. Plant Physiol 121 (1999) 333-343
55. Fitchette-Lainé A.C., Gomord V., Chekkafi A., and Faye L. Distribution of xylosylation and fucosylation in the plant Golgi-apparatus. Plant J 5 (1994) 673-682
56. Fitchette-Lainé A.C., Gomord V., Cabanes M., Michalski J.C., Saint Macary M., Foucher B., et al. N-glycans harboring the Lewis a epitope are expressed at the surface of plant cells. Plant J 12 (1997) 1411-1417
57. Floss D.M., Falkenburg D., and Conrad U. Production of vaccines and therapeutic antibodies for veterinary applications in transgenic plants: an overview. Transgenic Res 16 (2007) 315-332
58. Follet-Gueye M.L., Pagny S., Faye L., Gomord V., and Driouich A. An improved chemical fixation method suitable for immunogold localization of green fluorescent protein in the Golgi apparatus of tobacco bright yellow (BY-2) cells. J Histochem Cytochem 51 (2003) 931-940
59. Fötisch K., and Vieths S. N- and O-linked oligosaccharides of allergenic glycoproteins. Glycoconj J 18 (2001) 373-390
60. Frey A.D., Karg S.R., and Kallio P.T. Expression of rat β-(1,4)-N-acetylglucosaminyltransferase III in Nicotiana tabacum remodels the plant-specific N-glycosylation. Plant Biotechnol J 7 (2009) 33-48
61. Fujiyama K., Furukawa A., Katsura A., Misaki R., Omasa T., and Seki T. Production of mouse monoclonal antibody with galactose-extended sugar chain by suspension cultured tobacco BY2 cells expressing human beta-(1,4)-galactosyltransferase. Biochem Biophys Res Commun 358 (2007) 85-91
62. Fujiyama K., Palacpac N.Q., Sakai H., Kimura Y., Shinmyo A., Yoshida T., et al. In vivo conversion of a glycan to human compatible type by transformed tobacco cells. Biochem Biophys Res Commun 289 (2001) 553-557
63. Garber K. Biotech industry faces new bottleneck. Nat Biotechnol 19 (2001) 184-185
64. Gerngross T.U. Advances in the production of human therapeutic proteins in yeasts and filamentous fungi. Nat Biotechnol 22 (2004) 1409-1414
65. Giddings G., Allison G., Brooks D., and Carter A. Transgenic plants as factories for biopharmaceuticals. Nat Biotechnol 18 (2000) 1151-1155
66. Gils M., Kandzia R., Marillonnet S., Klimyuk V., and Gleba Y. High-yield production of authentic human growth hormone using a plant virus-based expression system. Plant Biotechnol J 3 (2005) 613-620
67. Girard L.S., Fabis M.J., Bastin M., Courtois D., Petiard V., and Koprowski H. Expression of a human anti-rabies virus monoclonal antibody in tobacco cell culture. Biochem Biophys Res Commun 345 (2006) 602-607
68. Giritch A., Marillonnet S., Engler C., van Eldik G., Botterman J., Klimyuk V., et al. Rapid high-yield expression of full-size IgG antibodies in plants coinfected with noncompeting viral vectors. Proc Natl Acad Sci U S A 103 (2006) 14701-14706
69. Golovkin M., Spitsin S., Andrianov V., Smirnov Y., Xiao Y.H., Pogrebnyak N., et al. Smallpox subunit vaccine produced in planta confers protection in mice. Proc Natl Acad Sci U S A 104 (2007) 6864-6869
70. Gomord V., and Faye L. 2004 Posttranslational modification of therapeutic proteins in plants. Curr Opin Plant Biol 7 (2004) 171-181
71. Gomord V., Denmat L.A., Fitchette-Lainé A.C., Satiat-Jeunemaitre B., Hawes C., and Faye L. The C-terminal HDEL sequence is sufficient for retention of secretory proteins in the endoplasmic reticulum (ER) but promotes vacuolar targeting of proteins that escape the ER. Plant J 11 (1997) 313-325
72. Gutiérrez-Ortega A., Sandoval-Montes C., de Olivera-Flores T.J., Santos-Argumedo L., and Gómez-Lim M.A. Expression of functional interleukin-12 from mouse in transgenic tomato plants. Transgenic Res 14 (2005) 877-885
73. Gutternigg M., Kretschmer-Lubich D., Paschinger K., Rendic D., Hader J., Geier P., et al. Biosynthesis of truncated N-linked oligosaccharides results from non-orthologous hexosaminidase-mediated mechanisms in nematodes, plants, and insects. J Biol Chem 282 (2007) 27825-27840
74. Hamers-Casterman C., Atarhouch T., Muyldermans S., Robinson G., Hamers C., Songa E.B., et al. Naturally occurring antibodies devoid of light chains. Nature 363 (1993) 446-448
75. Hiatt A., Cafferkey R., and Bowdish K. Production of antibodies in transgenic plants. Nature 342 (1989) 76-78
76. Hodoniczky J., Zheng Y.Z., and James D.C. Control of recombinant monoclonal antibody effector functions by Fc N-glycan remodeling in vitro. Biotechnol Prog 21 (2005) 1644-1652
77. Hohl U., Upmeier B., and Barz W. Growth and nicotinate biotransformation in batch cultured and airlift fermenter grown soybean cell-suspension cultures. Appl Microbiol Biotechnol 28 (1988) 319-323
78. Hood E.E., Witcher D.R., Maddock S., Meyer T., Baszczynski C., Bailey M., et al. Commercial production of avidin from transgenic maize: characterization of transformant, production, processing, extraction and purification. Mol Breed 3 (1997) 291-306
79. Hooker B.S., Lee J.M., and An G.H. Cultivation of plant-cells in a stirred vessel - effect of impeller design. Biotechnol Bioeng 35 (1990) 296-304
80. Houdebine L.M. Production of pharmaceutical proteins by transgenic animals. Comp Immunol Microbiol Infect Dis 32 (2009) 107-121
81. Huang J., Wu L., Yalda D., Adkins Y., Kelleher S.L., Crane M., et al. Expression of functional recombinant human lysozyme in transgenic rice cell culture. Transgenic Res 11 (2002) 229-239
82. Huang N. Invited presentation at "Third International Rushmore Conference on Enteric Diseases" in Rapid City, South Dakota, USA on September 29-October 1, 2005 entitled "Recombinant antimicrobial proteins, lactoferrin and lysozyme, produced in plants for control of enteric pathogens".
83. Iobst S.T., and Drickamer K. Selective sugar binding to the carbohydrate recognition domains of the rat hepatic and macrophage asialoglycoprotein receptors. J Biol Chem 271 (1996) 6686-6693
84. Ismaili A., Jalali-Javaran M., Rasaee M.J., Rahbarizadeh F., Forouzandeh-Moghadam M., and Memari H.R. Production and characterization of anti-(mucin MUCI) single-domain antibody in tobacco (Nicotiana tabacum cultivar Xanthi). Biotechnol Appl Biochem 47 (2007) 11-19
85. James E.A., Wang C.L., Wang Z.P., Reeves R., Shin J.H., Magnuson N.S., et al. Production and characterization of biologically active human GM-CSF secreted by genetically modified plant cells. Protein Expr Purif 19 (2000) 131-138
86. Jarvis D.L. Developing baculovirus-insect cell expression systems for humanized recombinant glycoprotein production. Virology 310 (2003) 1-7
87. Jenkins G.I. UV and blue light signal transduction in Arabidopsis. Plant Cell Environ 20 (1997) 773-778
88. Jenkins N., Parekh R.B., and James D.C. Getting the glycosylation right: implications for the biotechnology industry. Nat Biotechnol 14 (1996) 975-981
89. Jin C., Altmann F., Strasser R., Mach L., Schahs M., Kunert R., et al. A plant-derived human monoclonal antibody induces an anti-carbohydrate immune response in rabbits. Glycobiology 18 (2008) 235-241
90. Jobling S.A., Jarman C., Teh M.M., Holmberg N., Blake C., and Verhoeyen M.E. Immunomodulation of enzyme function in plants by single-domain antibody fragments. Nat Biotechnol 21 (2002) 77-80
91. Johnson K.D., and Chrispeels M.J. Substrate specificities of N-acetylglucosaminyl-transferases, fucosyltransferases, and xylosyltransferases that modify glycoproteins in the Golgi-apparatus of bean cotyledons. Plant Physiol 84 (1987) 1301-1308
92. Joshi L., and Lopez L.C. Bioprospecting in plants for engineered proteins. Curr Opin Plant Biol 8 (2005) 223-226
93. Kaiser J. Is the drought over for pharming?. Science 320 (2008) 473-475
94. Kapila J., De Rycke R., Van Montagu M., and Angenon G. An Agrobacterium-mediated transient gene expression system for intact leaves. Plant Sci 122 (1997) 101-108
95. Kapusta J., Modelska A., Figlerowicz M., Pniewski T., Letellier M., Lisowa O., et al. A plant-derived edible vaccine against hepatitis B virus. FASEB J 13 (1999) 1796-1799
96. Karasev A.V., Foulke S., Wellens C., Rich A., Shon K.J., Zwierzynski I., et al. Plant based HIV-1 vaccine candidate: Tat protein produced in spinach. Vaccine 23 (2005) 1875-1880
97. Karg S.R. Glycosylation engineering in plants: steps towards the production of humanized antibodies in Nicotiana tabacum. Insitute of Biotechnology, Zürich, ETH Zürich (2002)
98. Karg S.R., Frey A.D., Ferrara C., Streich D.K., Umaña P., and Kallio P.T. A small-scale method for the preparation of plant N-linked glycans from soluble proteins for analysis by MALDI-TOF mass spectrometry. Plant Physiol Biochem 47 (2009) 160-166
99. Karnoup A.S., Kuppannan K., and Young S.A. A novel HPLC-UV-MS method for quantitative analysis of protein glycosylation. J Chromatogr B Analyt Technol Biomed Life Sci 859 (2007) 178-191
100. Karnoup A.S., Turkelson V., and Anderson W.H. O-linked glycosylation in maize-expressed human IgA1. Glycobiology 15 (2005) 965-981
101. Kathuria S.R., Nath R., Pal R., Singh O., Fischer R., Lohiya N.K., et al. Functional recombinant antibodies against human chorionic gonadotropin expressed in plants. Curr Sci India 82 (2002) 1452-1457
102. Khoudi H., Laberge S., Ferullo J.M., Bazin R., Darveau A., Castonguay Y., et al. Production of a diagnostic monoclonal antibody in perennial alfalfa plants. Biotechnol Bioeng 64 (1999) 135-143
103. Ko K.S., Tekoah Y., Rudd P.M., Harvey D.J., Dwek R.A., Spitsin S., et al. Function and glycosylation of plant-derived antiviral monoclonal antibody. Proc Natl Acad Sci U S A 100 (2003) 8013-8018
104. Kogelberg H., Tolner B., Sharma S.K., Lowdell M.W., Qureshi U., Robson M., et al. Clearance mechanism of a mannosylated antibody-enzyme fusion protein used in experimental cancer therapy. Glycobiology 17 (2007) 36-45
105. Koprivova A., Stemmer C., Altmann F., Hoffmann A., Kopriva S., Gorr G., et al. Targeted knockouts of Physcomitrella lacking plantspecific immunogenic N-glycans. Plant Biotechnol J 2 (2004) 517-523
106. Kornfeld R., and Kornfeld S. Assembly of asparagine-linked oligosaccharides. Annu Rev Biochem 54 (1985) 631-664
107. Kwon T.H., Kim Y.S., Lee J.H., and Yang M.S. Production and secretion of biologically active human granulocyte-macrophage colony stimulating factor in transgenic tomato suspension cultures. Biotechnol Lett 25 (2003) 1571-1574
108. Lainé A.C., Gomord V., and Faye L. Xylose-specific antibodies as markers of subcompartmentation of terminal glycosylation in the Golgi-apparatus of sycamore cells. FEBS Lett 295 (1991) 179-184
109. Lamphear B.J., Jilka J.M., Kesl L., Welter M., Howard J.A., and Streatfield S.J. A corn-based delivery system for animal vaccines: an oral transmissible gastroenteritis virus vaccine boosts lactogenic immunity in swine. Vaccine 22 (2004) 2420-2424
110. Law R.D., Russell D.A., Thompson L.C., Schroeder S.C., Middle C.M., Tremaine M.T., et al. Biochemical limitations to high-level expression of humanized monoclonal antibodies in transgenic maize seed endosperm. BBA-Gen Subjects 1760 (2006) 1434-1444
111. Lawrence S. Billion dollar babies - biotech drugs as blockbusters. Nat Biotechnol 25 (2007) 380-382
112. Lee R.W., Strommer J., Hodgins D., Shewen P.E., Niu Y., and Lo R.Y. Towards development of an edible vaccine against bovine pneumonic pasteurellosis using transgenic white clover expressing a Mannheimia haemolytica A1 leukotoxin 50 fusion protein. Infect Immun 69 (2001) 5786-5793
113. Lee S.J., Park C.I., Park M.Y., Jung H.S., Ryu W.S., Lim S.M., et al. Production and characterization of human CTLA4Ig expressed in transgenic rice cell suspension cultures. Protein Expr Purif 51 (2007) 293-302
114. Leonard R., Costa G., Darrambide E., Lhernould S., Fleurat-Lessard P., Carlue M., et al. The presence of Lewis a epitopes in Arabidopsis thaliana glycoconjugates depends on an active alpha 4-fucosyltransferase gene. Glycobiology 12 (2002) 299-306
115. Leonard R., Kolarich D., Paschinger K., Altmann F., and Wilson I.B.H. A genetic and structural analysis of the N-glycosylation capabilities of rice and other monocotyledons. Plant Mol Biol 55 (2004) 631-644
116. Lerouge P., Cabanes-Macheteau M., Rayon C., Fischette-Lainé A.C., Gomord V., and Faye L. N-glycoprotein biosynthesis in plants: recent developments and future trends. Plant Mol Biol 38 (1998) 31-48
117. Lienard D., Dinh O.T., van Oort E., Van Overtvelt L., Bonneau C., Wambre E., et al. Suspension-cultured BY-2 tobacco cells produce and mature immunologically active house dust mite allergens. Plant Biotechnol J 5 (2007) 93-108
118. Lowe J.A., and Jones P. Biopharmaceuticals and the future of the pharmaceutical industry. Curr Opin Drug Discov Devel 10 (2007) 513-514
119. Ma J.K., Hiatt A., Hein M., Vine N.D., Wang F., Stabila P., et al. Generation and assembly of secretory antibodies in plants. Science 268 (1995) 716-719
120. Ma J.K., Hikmat B.Y., Wycoff K., Vine N.D., Chargelegue D., Yu L., et al. Characterization of a recombinant plant monoclonal secretory antibody and preventive immunotherapy in humans. Nat Med 4 (1998) 601-606
121. Ma J.K.C., Barros E., Bock R., Christou P., Dale P.J., Dix P.J., et al. Molecular farming for new drugs and vaccines - current perspectives on the production of pharmaceuticals in transgenic plants. EMBO Rep 6 (2005) 593-599
122. Magnuson N.S., Linzmaier P.M., Reeves R., An G.H., HayGlass K., and Lee J.M. Secretion of biologically active human interleukin-2 and interleukin-4 from genetically modified tobacco cells in suspension culture. Protein Expr Purif 13 (1998) 45-52
123. Marshall, B., www.molecularfarming.com, October 2007.
124. Marusic C., Nuttall J., Buriani G., Lico C., Lombardi R., Baschieri S., et al. Expression, intracellular targeting and purification of HIV Nef variants in tobacco cells. BMC Biotechnol 7 (2007) 12
125. Mason H.S., Lam D.M., and Arntzen C.J. Expression of hepatitis B surface antigen in transgenic plants. Proc Natl Acad Sci U S A 89 (1992) 11745-11749
126. Mayfield S.P., Franklin S.E., and Lerner R.A. Expression and assembly of a fully active antibody in algae. Proc Natl Acad Sci U S A 100 (2003) 438-442
127. McCormick A.A., Kumagai M.H., Hanley K., Turpen T.H., Hakim I., Grill L.K., et al. Rapid production of specific vaccines for lymphoma by expression of the tumor-derived single-chain Fv epitopes in tobacco plants. Proc Natl Acad Sci U S A 96 (1999) 703-708
128. McCormick A.A., Reddy S., Reinl S.J., Cameron T.I., Czerwinkski D.K., Vojdani F., et al. Plant-produced idiotype vaccines for the treatment of non-Hodgkin's lymphoma: safety and immunogenicity in a phase I clinical study. Proc Natl Acad Sci U S A 105 (2008) 10131-10136
129. Misaki R., Fujiyama K., and Seki T. Expression of human CMP-N-acetylneuraminic acid synthetase and CMP-sialic acid transporter in tobacco suspension-cultured cell. Biochem Biophys Res Commun 339 (2006) 1184-1189
130. Misaki R., Kimura Y., Palacpac N.Q., Yoshida S., Fujiyama K., and Seki T. Plant cultured cells expressing human beta-(1,4)-galactosyltransferase secrete glycoproteins with galactose-extended N-linked glycans. Glycobiology 13 (2003) 199-205
131. Morrow K.J. Glycosylation in scale-up of antibody production - new field of glycomics on uncertain path. Genet Eng News 22 (2002) 54-57
132. Mulder H., Dideberg F., Schachter H., Spronk B.A., Dejongbrink M., Kamerling J.P., et al. In the biosynthesis of N-glycans in connective tissue of the snail Lymnaea stagnalis of incorporation GlcNAc by beta-2GlcNAc-Transferase-I is an essential prerequisite for the action of beta-2GlcNAc-Transferase-II and beta-2xyl-Transferase. Eur J Biochem 232 (1995) 272-283
133. Nagata T., Nemoto Y., and Hasezawa S. Tobacco BY-2 cell line as the "HeLa" cell in the cell biology of higher plants. Int Rev Cytol 132 (1992) 1-30
134. Narasimhan S. Control of glycoprotein-synthesis.7. Udp-GlcNAc-Glycopeptide beta-4-N-acetylglucosaminyltransferase-III, an enzyme in hen oviduct which adds GlcNAc in beta-(1-4) linkage to the beta-linked mannose of the trimannosyl core of N-glycosyl oligosaccharides. J Biol Chem 257 (1982) 10235-10242
135. Navazio L., Miuzzo M., Royle L., Baldan B., Varotto S., Merry A.H., et al. Monitoring endoplasmic reticulum-to-Golgi traffic of a plant calreticulin by protein glycosylation analysis. Biochemistry - US 41 (2002) 14141-14149
136. Nuttall J., Ma J.K.C., and Frigerio L. A functional antibody lacking N-linked glycans is efficiently folded, assembled and secreted by tobacco mesophyll protoplasts. Plant Biotechnol J 3 (2005) 497-504
137. O'Keefe B.R., Vojdani F., Buffa V., Shattock R.J., Montefiori D.C., Bakke J., et al. Scaleable manufacture of HIV-1 entry inhibitor griffithsin and validation of its safety and efficacy as a topical microbicide component. Proc Natl Acad Sci U S A 106 (2009) 6099-6104
138. Owen M., Gandecha A., Cockburn B., and Whitelam G. Synthesis of a functional anti-phytochrome single-chain Fv protein in transgenic tobacco. Biotechnology 10 (1992) 790-794
139. Oxley D., and Bacic A. Microheterogeneity of N-glycosylation on a stylar self-incompatibility glycoprotein of Nicotiana alata. Glycobiology 5 (1995) 517-523
140. Paccalet T., Bardor M., Rihouey C., Delmas F., Chevalier C., D'Aoust M.A., et al. Engineering of a sialic acid synthesis pathway in transgenic plants by expression of bacterial Neu5Ac-synthesizing enzymes. Plant Biotechnol J 5 (2007) 16-25
141. Pagny S., Bouissonnie F., Sarkar M., Follet-Gueye M.L., Driouich A., Schachter H., et al. Structural requirements for Arabidopsis beta-(1,2)-xylosyltransferase activity and targeting to the Golgi. Plant J 33 (2003) 189-203
142. Palacpac N.Q., Yoshida S., Sakai H., Kimura Y., Fujiyama K., Yoshida T., et al. Stable expression of human beta-(1,4)-galactosyltransferase in plant cells modifies N-linked glycosylation patterns. Proc Natl Acad Sci U S A 96 (1999) 4692-4697
143. Parmenter D.L., Boothe J.G., van Rooijen G.J., Yeung E.C., and Moloney M.M. Production of biologically active hirudin in plant seeds using oleosin partitioning. Plant Mol Biol 29 (1995) 1167-1180
144. Perrin Y., Vaquero C., Gerrard I., Sack M., Drossard J., Stoger E., et al. Transgenic pea seeds as bioreactors for the production of a single-chain Fv fragment (scFV) antibody used in cancer diagnosis and therapy. Mol Breed 6 (2000) 345-352
145. Petruccelli S., Otegui M.S., Lareu F., Dinh O.T., Fitchette A.C., Circosta A., et al. A KDEL-tagged monoclonal antibody is efficiently retained in the endoplasmic reticulum in leaves, but is both partially secreted and sorted to protein storage vacuoles in seeds. Plant Biotechnol J 4 (2006) 511-527
146. Pujol M., Gavilondo J., Ayala M., Rodríguez M., González E.M., and Pérez L. Fighting cancer with plant-expressed pharmaceuticals. Trends Biotechnol. 25 (2007) 455-459
147. Rademacher T., Sack M., Arcalis E., Stadlmann J., Balzer S., Altmann F., et al. Recombinant antibody 2G12 produced in maize endosperm efficiently neutralizes HIV-1 and contains predominantly single-GlcNAc N-glycans. Plant Biotechnol J 6 (2008) 189-201
148. Ramessar K., Rademacher T., Sack M., Stadlmann J., Platis D., Stiegler G., et al. Cost-effective production of a vaginal protein microbicide to prevent HIV transmission. Proc Natl Acad Sci U S A 105 (2008) 3727-3732
149. Ramírez N., Ayala M., Lorenzo D., Palenzuela D., Herrera L., Doreste V., et al. Expression of a single-chain Fv antibody fragment specific for the hepatitis B surface antigen in transgenic tobacco plants. Transgenic Res 11 (2002) 61-64
150. Rodríguez M., Ramírez N.I., Ayala M., Freyre F., Pérez L., Triguero A., et al. Transient expression in tobacco leaves of an aglycosylated recombinant antibody against the epidermal growth factor receptor. Biotechnol Bioeng 89 (2005) 188-194
151. Rouwendal G.J.A., Wuhrer M., Florack D.E.A., Koeleman C.A.M., Deelder A.M., Bakker H., et al. Efficient introduction of a bisecting GlcNAc residue in tobacco N-glycans by expression of the gene encoding human N-acetylglucosaminyltransferase III. Glycobiology 17 (2007) 334-344
152. Russell D.A., Spatola L.A., Dian T., Paradkar V.M., Dufield D.R., Carroll J.A., et al. Host limits to accurate human growth hormone production in multiple plant systems. Biotechnol Bioeng 89 (2005) 775-782
153. Saint-Jore-Dupas C., Faye L., and Gomord V. From planta to pharma with glycosylation in the toolbox. Trends Biotechnol 25 (2007) 317-323
154. Schachter H., Narasimhan S., Gleeson P., and Vella G. Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type. Methods Enzymol 98 (1983) 98-134
155. Schäffer C., Graninger M., and Messner P. Prokaryotic glycosylation. Proteomics 1 (2001) 248-261
156. Schähs M., Strasser R., Stadlmann J., Kunert R., Rademacher T., and Steinkellner H. Production of a monoclonal antibody in plants with a humanized N-glycosylation pattern. Plant Biotechnol J 5 (2007) 657-663
157. Schmidt F.R. Recombinant expression systems in the pharmaceutical industry. Appl Microbiol Biotechnol 65 (2004) 363-372
158. Seveno M., Bardor M., Paccalet T., Gomord V., Lerouge P., and Faye L. Glycoprotein sialylation in plants?. Nat Biotechnol 22 (2004) 1351-1352 author reply 1352-3
159. Shaaltiel Y., Bartfeld D., Hashmueli S., Baum G., Brill-Almon E., Galili G., et al. Production of glucocerebrosidase with terminal mannose glycans for enzyme replacement therapy of Gaucher's disease using a plant cell system. Plant Biotechnol J 5 (2007) 579-590
160. Shah M.M., Fujiyama K., Flynn C.R., and Joshi L. Sialylated endogenous glycoconjugates in plant cells. Nat Biotechnol 21 (2003) 1470-1471
161. Sharma A.K., and Sharma M.K. Plants as bioreactors: recent developments and emerging opportunities. Biotechnol Adv (2009) 10.1016/j.biotechadv.2009.06.004
162. Shattock R.J., and Moore J.P. Inhibiting sexual transmission of HIV-1 infection. Nat Rev Microbiol 1 (2003) 25-34
163. Shchelkunov S.N., Salyaev R.K., Pozdnyakov S.G., Rekoslavskaya N.I., Nesterov A.E., Ryzhova T.S., et al. Immunogenicity of a novel, bivalent, plant-based oral vaccine against hepatitis B and human immunodeficiency viruses. Biotechnol Lett 28 (2006) 959-967
164. Shields R.L., Lai J., Keck R., O'Connell L.Y., Hong K., Meng Y.G., et al. Lack of fucose on human IgG1 N-linked oligosaccharide improves binding to human Fc gamma RIII and antibody-dependent cellular toxicity. J Biol Chem 277 (2002) 26733-26740
165. Sijmons P.C., Dekker B.M., Schrammeijer B., Verwoerd T.C., van den Elzen P.J., and Hoekema A. Production of correctly processed human serum albumin in transgenic plants. Biotechnology 8 (1990) 217-221
166. Simmons L.C., Reilly D., Klimowski L., Raju T.S., Meng G., Sims P., et al. Expression of full-length immunoglobulins in Escherichia coli: rapid and efficient production of aglycosylated antibodies. J Immunol Methods 263 (2002) 133-147
167. Soreq H., and Seidman S. Acetylcholinesterase - new roles for an old actor. Nat Rev, Neurosci 2 (2001) 294-302
168. Sorrentino A., Schillberg S., Fischer R., Rao R., Porta R., and Mariniello L. Recombinant human tissue transglutaminase produced into tobacco suspension cell cultures is active and recognizes autoantibodies in the serum of coeliac patients. Int J Biochem Cell Biol 37 (2005) 842-851
169. Sourrouille C., Marquet-Blouin E., D'Aoust M.A., Kiefer-Meyer M.C., Seveno M., Pagny-Salehabadi S., et al. Down-regulated expression of plant-specific glycoepitopes in alfalfa. Plant Biotechnol J 6 (2008) 702-721
170. Sparrow P.A.C., Irwin J.A., Dale P.J., Twyman R.M., and Ma J.K.C. Pharma-planta: road testing the developing regulatory guidelines for plant-made pharmaceuticals. Transgenic Res 16 (2007) 147-161
171. Spiro R.G. Protein glycosylation: nature, distribution, enzymatic formation, and disease implications of glycopeptide bonds. Glycobiology 12 (2002) 43R-56R
172. Spök A. Molecular farming on the rise-GMO regulators still walking a tightrope. Trends Biotechnol 25 (2007) 74-82
173. Stöger E., Vaquero C., Torres E., Sack M., Nicholson L., Drossard J., et al. Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies. Plant Mol Biol 42 (2000) 583-590
174. Strasser R., Altmann F., Mach L., Glossl J., and Steinkellner H. Generation of Arabidopsis thaliana plants with complex N-glycans lacking beta-(1,2)-linked xylose and core alpha-(1,3)-linked fucose. FEBS Lett 561 (2004) 132-136
175. Strasser R., Bondili J.S., Vavra U., Schoberer J., Svoboda B., Glossl J., et al. A unique beta-(1,3)-galactosyltransferase is indispensable for the biosynthesis of N-Glycans containing Lewis a structures in Arabidopsis thaliana. Plant Cell 19 (2007) 2278-2292
176. Strasser R., Mucha J., Mach L., Altmann F., Wilson I.B.H., Glossl J., et al. Molecular cloning and functional expression of beta-(1,2)-xylosyltransferase cDNA from Arabidopsis thaliana. FEBS Lett 472 (2000) 105-108
177. Strasser R., Mucha J., Schwihla H., Altmann F., Glossl J., and Steinkellner H. Molecular cloning and characterization of cDNA coding for beta-(1,2)-N-acetylglucosaminyltransferase I (GlcNAc-TI) from Nicotiana tabacum. Glycobiology 9 (1999) 779-785
178. Strasser R., Steinkellner H., Boren M., Altmann F., Mach L., Glossl J., et al. Molecular cloning of cDNA encoding N-acetylglucosaminyltransferase II from Arabidopsis thaliana. Glycoconj J 16 (1999) 787-791
179. Strasser R., Schoberer J., Jin C.S., Glossl J., Mach L., and Steinkellner H. Molecular cloning and characterization of Arabidopsis thaliana Golgi alpha-mannosidase II, a key enzyme in the formation of complex N-glycans in plants. Plant J 45 (2006) 789-803
180. Strasser R., Stadlmann J., Schahs M., Stiegler G., Quendler H., Mach L., et al. Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure. Plant Biotechnol J 6 (2008) 392-402
181. Streatfield S.J., Lane J.R., Brooks C.A., Barker D.K., Poage M.L., Mayor J.M., et al. Corn as a production system for human and animal vaccines. Vaccine 21 (2003) 812-815
182. Tacket C.O., Mason H.S., Losonsky G., Clements J.D., Levine M.M., and Arntzen C.J. Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato. Nat Med 4 (1998) 607-609
183. Tacket C.O., Mason H.S., Losonsky G., Estes M.K., Levine M.M., and Arntzen C.J. Human immune responses to a novel Norwalk virus vaccine delivered in transgenic potatoes. J Infect Dis 182 (2000) 302-305
184. Tan L., Qiu F., Lamport D.T., and Kieliszewski M.J. Structure of a hydroxyproline (Hyp)-arabinogalactan polysaccharide from repetitive Ala-Hyp expressed in transgenic Nicotiana tabacum. J Biol Chem 279 (2004) 13156-13165
185. Tavladoraki P., Benvenuto E., Trinca S., De Martinis D., Cattaneo A., and Galeffi P. Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack. Nature 366 (1993) 469-472
186. Tekoah Y., Ko K.S., Koprowski H., Harvey D.J., Wormald M.R., Dwek R.A., et al. Controlled glycosylation of therapeutic antibodies in plants. Arch Biochem Biophys 426 (2004) 266-278
187. Terashima M., Murai Y., Kawamura M., Nakanishi S., Stoltz T., Chen L., et al. Production of functional human alpha 1-antitrypsin by plant cell culture. Appl Microbiol Biotechnol 52 (1999) 516-523
188. Terrier B., Courtois D., Henault N., Cuvier A., Bastin M., Aknin A., et al. Two new disposable bioreactors for plant cell culture: the wave and undertow bioreactor and the slug bubble bioreactor. Biotechnol Bioeng 96 (2007) 914-923
189. Tezuka K., Hayashi M., Ishihara H., Akazawa T., and Takahashi N. Studies on synthetic pathway of xylose-containing N-linked oligosaccharides deduced from substrate specificities of the processing enzymes in sycamore cells (Acer pseudoplatanus L.). Eur J Biochem 203 (1992) 401-413
190. Thanavala Y., Mahoney M., Pal S., Scott A., Richter L., Natarajan N., et al. Immunogenicity in humans of an edible vaccine for hepatitis B. Proc Natl Acad Sci U S A 102 (2005) 3378-3382
191. Thiel K.A. Biomanufacturing, from bust to boom...to bubble?. Nat Biotechnol 22 (2004) 1365-1372
192. Tiwari S., Verma P.C., Singh P.K., and Tuli R. Plants as bioreactors for the production of vaccine antigens. Biotechnol Adv 27 (2009) 449-467
193. Torres E., Vaquero C., Nicholson L., Sack M., Stoger E., Drossard J., et al. Rice cell culture as an alternative production system for functional diagnostic and therapeutic antibodies. Transgenic Res 8 (1999) 441-449
194. Tregoning J.S., Nixon P., Kuroda H., Svab Z., Clare S., Bowe F., et al. Expression of tetanus toxin fragment C in tobacco chloroplasts. Nucleic Acids Res 31 (2003) 1174-1179
195. Triguero A., Cabrera G., Cremata J.A., Yuen C.T., Wheeler J., and Ramírez N.I. Plant-derived mouse IgG monoclonal antibody fused to KDEL endoplasmic reticulum-retention signal is N-glycosylated homogeneously throughout the plant with mostly high-mannose-type N-glycans. Plant Biotechnol J 3 (2005) 449-457
196. Tuboly T., Yu W., Bailey A., Degrandis S., Du S., Erickson L., et al. Immunogenicity of porcine transmissible gastroenteritis virus spike protein expressed in plants. Vaccine 18 (2000) 2023-2028
197. Umaña P., Jean-Mairet J., Moudry R., Amstutz H., and Bailey J.E. Engineered glycoforms of an antineuroblastoma IgG1 with optimized antibody-dependent cellular cytotoxic activity. Nat Biotechnol 17 (1999) 176-180
198. van Tetering A., Schiphorst W., van den Eijnden D.H., and van Die I. Characterization of a core alpha 1 -> 3-fucosyltransferase from the snail Lymnaea stagnalis that is involved in the synthesis of complex-type N-glycans. FEBS Lett 461 (1999) 311-314
199. Vaquero C., Sack M., Chandler J., Drossard J., Schuster F., Monecke M., et al. Transient expression of a tumor-specific single-chain fragment and a chimeric antibody in tobacco leaves. Proc Natl Acad Sci U S A 96 (1999) 11128-11133
200. Varki A. Biological Roles of Oligosaccharides - all of the theories are correct. Glycobiology 3 (1993) 97-130
201. Vermij P. Edible vaccines not ready for main course. Nat Med 10 (2004) 881
202. Vermij P., and Waltz E. USDA approves the first plant-based vaccine. Nat Biotechnol 24 (2006) 233-234
203. Vézina L.P., Faye L., Lerouge P., D'Aoust M.A., Marquet-Blouin E., Burel C., et al. Transient co-expression for fast and high-yield production of antibodies with human-like N-glycans in plants. Plant Biotechnol J 7 (2009) 442-455
204. Wagner R., Geyer H., Geyer R., and Klenk H.D. N-acetyl-beta-glucosaminidase accounts for differences in glycosylation of influenza virus hemagglutinin expressed in insect cells from a Baculovirus vector. J Virol 70 (1996) 4103-4109
205. Walsh G. Biopharmaceutical benchmarks 2006. Nat Biotechnol 24 (2006) 769
206. Warnock D., Bai X.M., Autote K., Gonzales J., Kinealy K., Yan B., et al. In vitro galactosylation of human IgG at 1 kg scale using recombinant galactosyltransferase. Biotechnol Bioeng 92 (2005) 831-842
207. Wee E.G.T., Sherrier D.J., Prime T.A., and Dupree P. Targeting of active sialyltransferase to the plant Golgi apparatus. Plant Cell 10 (1998) 1759-1768
208. Weise A., Altmann F., Rodriguez-Franco M., Sjoberg E.R., Baumer W., Launhardt H., et al. High-level expression of secreted complex glycosylated recombinant human erythropoietin in the Physcomitrella Delta-fuc-t Delta-xyl-t mutant. Plant Biotechnol J 5 (2007) 389-401
209. Wilson I.B.H. Glycosylation of proteins in plants and invertebrates. Curr Opin Struck Biol 12 (2002) 569-577
210. Woodard S.L., Mayor J.M., Bailey M.R., Barker D.K., Love R.T., Lane J.R., et al. Maize (Zea mays)-derived bovine trypsin: characterization of the first large-scale, commercial protein product from transgenic plants. Biotechnol Appl Biochem 38 (2003) 123-130
211. Wopereis S., Lefeber D.J., Morava E., and Wevers R.A. Mechanisms in protein O-glycan biosynthesis and clinical and molecular aspects of protein O-glycan biosynthesis defects: a review. Clin Chem 52 (2006) 574-600
212. Wright A., and Morrison S.L. Effect of altered C(H)2-associated carbohydrate structure on the functional properties and in-vivo fate of chimeric mouse-human immunoglobulin G1. J Exp Med 180 (1994) 1087-1096
213. Wright A., and Morrison S.L. Effect of glycosylation on antibody function: implications for genetic engineering. Trends Biotechnol 15 (1997) 26-32
214. Yang P.L., Wang Y.R., Bai Y.G., Meng K., Luo H.Y., Yuan T.Z., et al. Expression of xylanase with high specific activity from Streptomyces olivaceoviridis A1 in transgenic potato plants (Solanum tuberosum L.). Biotechnol Lett 29 (2007) 659-667
215. Yin J.C., Li G.X., Ren X.F., and Herrler G. Select what you need: a comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes. J Biotechnol 127 (2007) 335-347
216. Yusibov V., Hooper D.C., Spitsin S.V., Fleysh N., Kean R.B., Mikheeva T., et al. Expression in plants and immunogenicity of plant virus-based experimental rabies vaccine. Vaccine 20 (2002) 3155-3164
217. Zavaleta N., Figueroa D., Rivera J., Sanchez J., Alfaro S., and Lonnerdal B. Efficacy of rice-based oral rehydration solution containing recombinant human lactoferrin and lysozyme in Peruvian children with acute diarrhea. J Pediatr Gastroenterol Nutr 44 (2007) 258-264
218. Zeleny R., Kolarich D., Strasser R., and Altmann F. Sialic acid concentrations in plants are in the range of inadvertent contamination. Planta 224 (2006) 222-227
219. Zeng Y.C., Bannon G., Thomas V.H., Rice K., Drake R., and Elbein A. Purification and specificity of beta 1,2-xylosyltransferase, an enzyme that contributes to the allergenicity of some plant proteins. J Biol Chem 272 (1997) 31340-37
220. Zhang G.G., Rodrigues L., Rovinski B., and White K.A. Production of HIV-1 p24 protein in transgenic tobacco plants. Mol Biotechnol 20 (2002) 131-136
221. Zhu Z., Hughes K.W., Huang L., Sun B.L., Liu C.M., Li Y., et al. Expression of human alpha-interferon cDNA in transgenic rice plants. Plant Cell Tissue Organ cult 36 (1994) 197-204