Metabolic control of recombinant protein N-glycan processing in NS0 and CHO cells

Biotechnology and Bioengineering

Volumn 73, Issue 3, 2001, Pages 188-202

  Baker, Kym N ^a   Rendall, Mark H ^a   Hills, Anna E ^a   Hoare, Michael ^a   Freedman, Robert B ^a   James, David C ^a,b  

^a UNIVERSITY OF KENT   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

galactose; Chinese hamster ovary (CHO) cells; Glycosylation; High performance liquid chromatography (HPLC); Murine myeloma cells; Nucleotide sugar; Sialic acid

Indexed keywords

CELLS; ENZYME KINETICS; PROTEINS; TISSUE;

RECOMBINANT PROTEINS;

METABOLISM;

GALACTOSE; GLUCOSAMINE; GLYCAN DERIVATIVE; HEXOSAMINE; MANNOSAMINE; N ACETYLMANNOSAMINE; N ACETYLNEURAMINIC ACID; N GLYCOLOYLNEURAMINIC ACID; OLIGOSACCHARIDE; RECOMBINANT PROTEIN; SIALIC ACID; SIALYLTRANSFERASE; TISSUE INHIBITOR OF METALLOPROTEINASE 1; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE N ACETYLHEXOSAMINE;

ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; DERIVATIZATION; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMUNOAFFINITY CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLIC REGULATION; MOUSE; MYELOMA CELL; NONHUMAN; PROTEIN PROCESSING; SIALYLATION;

ANIMALS; BIOREACTORS; CELL DIVISION; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; GALACTOSE; GLUCOSAMINE; GLYCOSYLATION; HEXOSAMINES; MICE; N-ACETYLNEURAMINIC ACID; NUCLEOTIDES; POLYSACCHARIDES; RECOMBINANT PROTEINS; SIALYLTRANSFERASES; TISSUE INHIBITOR OF METALLOPROTEINASE-1; TUMOR CELLS, CULTURED;

CRICETINAE; CRICETULUS GRISEUS; MURINAE;

EID: 0035810656     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.1051     Document Type: Article

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