Production of a monoclonal antibody in plants with a humanized N-glycosylation pattern

Plant Biotechnology Journal

Volumn 5, Issue 5, 2007, Pages 657-663

  Schähs, Matthias ^a   Strasser, Richard ^a   Stadlmann, Johannes ^a   Kunert, Renate ^b   Rademacher, Thomas ^c   Steinkellner, Herta ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

^c RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

1,2 xylose and core 1,3 fucose; Antibodies; Plant glycan engineering; Recombinant proteins

Indexed keywords

EPITOPE; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY; POLYSACCHARIDE; RECOMBINANT PROTEIN;

ANIMAL; ARABIDOPSIS; ARTICLE; CHEMISTRY; CHO CELL; CRICETULUS; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE DELETION; GENETICS; GLYCOSYLATION; HAMSTER; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; PLANT LEAF; TRANSGENIC PLANT;

ANIMALS; ANTIBODIES, MONOCLONAL; ARABIDOPSIS; CHO CELLS; CRICETINAE; CRICETULUS; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; GENE DELETION; GLYCOSYLATION; HUMANS; IMMUNOGLOBULIN G; PLANT LEAVES; PLANTS, GENETICALLY MODIFIED; POLYSACCHARIDES; RECOMBINANT PROTEINS;

ARABIDOPSIS; ARABIDOPSIS THALIANA; CRICETULUS GRISEUS; MAMMALIA;

EID: 34547568959     PISSN: 14677644     EISSN: 14677652     Source Type: Journal    
DOI: 10.1111/j.1467-7652.2007.00273.x     Document Type: Article

References (25)

1. Bakker, H., Bardor, M., Molthoff, J.W., Gomord, V., Elbers, I., Stevens, L.H., Jordi, W., Lommen, A., Faye, L., Lerouge, P. Bosch, D. (2001) Galactose-extended glycans of antibodies produced by transgenic plants. Proc. Natl. Acad. Sci. USA, 98, 2899 2904.
2. Bakker, H., Rouwendal, G.J., Karnoup, A.S., Florack, D.E., Stoopen, G.M., van Helsper, J.P., van Ree, R., Die, I. Bosch, D. (2006) An antibody produced in tobacco expressing a hybrid beta-1,4-galactosyltransferase is essentially devoid of plant carbohydrate epitopes. Proc. Natl. Acad. Sci. USA, 103, 7577 7582.
3. Bardor, M., Faveeuw, C., Fitchette, A.C., Gilbert, D., Galas, L., Trottein, F., Faye, L. Lerouge, P. (2003) Immunoreactivity in mammals of two typical plant glyco-epitopes, core alpha (1,3)-fucose and core xylose. Glycobiology, 13, 427 434.
4. Bencurova, M., Hemmer, W., Focke-Tejkl, M., Wilson, I.B. Altmann, F. (2004) Specificity of IgG and IgE antibodies against plant and insect glycoprotein glycans determined with artificial glycoforms of human transferrin. Glycobiology, 14, 457 466.
5. Cox, K.M., Sterling, J.D., Regan, J.T., Gasdaska, J.R., Frantz, K.K., Peele, C.G., Black, A., Passmore, D., Moldovan-Loomis, C., Srinivasan, M., Cuison, S., Cardarelli, P.M. Dickey, L.F. (2006) Glycan optimization of a human monoclonal antibody in the aquatic plant Lemna minor. Nat. Biotechnol. 24, 1591 1597.
6. De Jaeger, G., Scheffer, S., Jacobs, A., Zambre, M., Zobell, O., Goossens, A., Depicker, A. Angenon. G. (2002) Boosting heterologous protein production in transgenic dicotyledonous seeds using Phaseolus vulgaris regulatory sequences. Nat. Biotechnol. 20, 1265 1268.
7. Faye, L., Gomord, V., Fitchette-Laine, A.C. Chrispeels, M.J. (1993) Affinity purification of antibodies specific for Asn-linked glycans containing alpha 1→3 fucose or beta 1→2 xylose. Anal. Biochem. 209, 104 108.
8. Giritch, A., Marillonnet, S., Engler, C., van Eldik, G., Botterman, J., Klimyuk, V. Gleba, Y. (2006) Rapid high-yield expression of full-size IgG antibodies in plants coinfected with noncompeting viral vectors. Proc. Natl. Acad. Sci. USA, 103, 14 701 14 706.
9. Jach, G., Binot, E., Frings, S., Luxa, K. Schell, J. (2001) Use of red fluorescent protein from Discosoma sp. (dsRED) as a reporter for plant gene expression. Plant J. 28, 483 491.
10. Jefferis, R. (2005) Glycosylation of recombinant antibody therapeutics. Biotechnol. Prog. 21, 11 16.
11. Jin, C., Bencurova, M., Borth, N., Ferko, B., Jensen-Jarolim, E., Altmann, F. Hantusch, B. (2006) Immunoglobulin G specifically binding plant N-glycans with high affinity could be generated in rabbits but not in mice. Glycobiology, 16, 349 357.
12. Ko, K., Tekoah, Y., Rudd, P.M., Harvey, D.J., Dwek, R.A., Spitsin, S., Hanlon, C.A., Rupprecht, C., Dietzschold, B., Golovkin, M. Koprowski, H. (2003) Function and glycosylation of plant-derived antiviral monoclonal antibody. Proc. Natl. Acad. Sci. USA, 100, 8013 8018.
13. Kolarich, D. Altmann, F. (2000) N-Glycan analysis by matrix-assisted laser desorption/ionization mass spectrometry of electrophoretically separated nonmammalian proteins: application to peanut allergen Ara h 1 and olive pollen allergen Ole e 1. Anal. Biochem. 285, 64 75.
14. Kurosaka, A., Yano, A., Itoh, N., Kuroda, Y., Nakagawa, T. Kawasaki, T. (1991) The structure of a neural specific carbohydrate epitope of horseradish peroxidase recognized by anti-horseradish peroxidase antiserum. J. Biol. Chem. 266, 4168 4172.
15. Leonard, R., Rendic, D., Wilson, I.B.H. Altmann, F. (2005) Adaptation of the 'in gel release method' to glycom studies from a very little amount of material. Glycoconjugate J. 22, 238.
16. Ma, J.K., Hiatt, A., Hein, M., Vine, N.D., Wang, F., van Stabila, P., Dolleveerd. C., Mostov, K. Lehner, T. (1995) Generation and assembly of secretory antibodies in plants. Science, 268, 716 719.
17. Marillonnet, S., Giritch, A., Gils, M., Kandzia, R., Klimyuk, V. Gleba, Y. (2004) In planta engineering of viral RNA replicons: efficient assembly by recombination of DNA modules delivered by Agrobacterium. Proc. Natl. Acad. Sci. USA, 101, 6852 6857.
18. Marillonnet, S., Thoeringer, C., Kandzia, R., Klimyuk, V. Gleba, Y. (2005) Systemic Agrobacterium tumefaciens-mediated transfection of viral replicons for efficient transient expression in plants. Nat. Biotechnol. 23, 718 723.
19. Palacpac, N.Q., Yoshida, S., Sakai, H., Kimura, Y., Fujiyama, K., Yoshida, T. Seki, T. (1999) Stable expression of human beta1,4- galactosyltransferase in plant cells modifies N-linked glycosylation patterns. Proc. Natl. Acad. Sci. USA, 96, 4692 4697.
20. Schouten, A., Roosien, J., van Engelen, F.A., de Jong, G.A., Borst-Vrenssen, A.W., Zilverentant, J.F., Bosch, D., Stiekema, W.J., Gommers, F.J., Schots, A. Bakker, J. (1996) The C-terminal KDEL sequence increases the expression level of a single-chain antibody designed to be targeted to both the cytosol and the secretory pathway in transgenic tobacco. Plant Mol. Biol. 30, 781 793.
21. Shields, R.L., Lai, J., Keck, R., O'Connell, L.Y., Hong, K., Meng, Y.G., Weikert, S.H. Presta, L.G. (2001) Lack of fucose on human IgG1 N-linked oligosaccharide improves binding to human Fcgamma RIII and antibody-dependent cellular toxicity. J. Biol. Chem. 277, 26 733 26 740.
22. Strasser, R., Altmann, F., Mach, L., Glossl, J. Steinkellner, H. (2004) Generation of Arabidopsis thaliana plants with complex N-glycans lacking beta 1,2-linked xylose and core alpha 1,3-linked fucose. FEBS Lett. 561, 132 136.
23. Trkola, A., Pomales, A.B., Yuan, H., Korber, B., Maddon, P.J., Allaway, G.P., Katinger, H., Barbas, C.F., Burton, D.R., Ho, D.D. Moore, J.P. (1995) Cross-clade neutralization of primary isolates of human immunodeficiency virus type 1 by human monoclonal antibodies and tetrameric CD4-IgG. J. Virol. 69, 6609 6617.
24. Trkola, A., Purtscher, M., Muster, T., Ballaun, C., Buchacher, A., Sullivan, N., Srinivasan, K., Sodroski, J., Moore, J.P. Katinger, H. (1996) Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. J. Virol. 70, 1100 1108.
25. Van Droogenbroeck, B., Cao, J., Stadlmann, J., Altmann, F., Colanesi, S., Hillmer, S., Robinson, D.G., Van Lerberge, E., Terryn, N., Van Montagu, M., Liang, M., Depicker, A. De Jaeger, G. (2007) Aberrant localization and underglycosylation of highly accumulating single-chain Fv-Fc antibodies in transgenic Arabidopsis seeds. Proc. Natl Acad. Sci. USA, 104, 1430 1435.