Production and secretion of biologically active human granulocyte-macrophage colony stimulating factor in transgenic tomato suspension cultures

Biotechnology Letters

Volumn 25, Issue 18, 2003, Pages 1571-1574

  Kwon, Tae Ho ^a   Kim, Young Sook ^a   Lee, Jae Hwa ^b   Yang, Moon Sik ^a  

^a Chonbuk National University   (South Korea)

^b Silla University   (South Korea)

Author keywords

Granulocyte macrophage colony stimulating factor; Plant suspension culture; Recombinant protein; Tomato

Indexed keywords

GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; RECOMBINANT PROTEIN;

ARTICLE; BIOSYNTHESIS; CELL CULTURE; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; METABOLISM; METHODOLOGY; PLANT LEAF; PROTEIN ENGINEERING; SECRETION; TISSUE DISTRIBUTION; TOMATO; TRANSGENIC PLANT;

CELLS, CULTURED; GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; HUMANS; LYCOPERSICON ESCULENTUM; PLANT LEAVES; PLANTS, GENETICALLY MODIFIED; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; TISSUE DISTRIBUTION;

AGROBACTERIUM; LYCOPERSICON; LYCOPERSICON ESCULENTUM;

EID: 0141565118     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1025409927790     Document Type: Article

References (16)

1. Alwine JC, Kemp DJ, Stark GR (1977) Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc. Natl. Acad.Sci. USA 74: 5350-5354.
2. Chiou CJ, Wu MC (1990) Expression of human granulocyte-macrophage colony-stimulating factor gene in insect cells by a baculovirus vector. FEBS Lett. 259: 249-253.
3. Doran PM (2000) Foreign protein production in plant tissue cultures. Curr. Opin. Biotechnol. 11: 199-204.
4. Herbers K, Sonnewald U (1999) Production of new/modified proteins in transgenic plants. Curr. Opin. Biotechnol. 10: 163-168.
5. James EA, Eang C, Wang Z, Reeves R (2000) Production and characterization of biologically active human GM-CSF secreted by genetically modified plant cells. Protein Express. Purif. 19: 131-138.
6. Kim MJ, Kwon TH, Jang YS, Yang MS, Kim DH (2000) Expression of murine GM-CSF in recombinant Aspergillus niger. J. Microbiol. Biotech. 10: 287-292.
7. Kwon TH, Seo JE, Kim J, Lee JH, Jang YS, Yang MS (2003a) Expression and secretion of the heterodimeric protein interleukin-12 in plant cell suspension culture. Biotech. Bioeng. 81: 870-875.
8. Kwon TH, Shin YM, Kim YS, Jang YS, Yang MS (2003b) Secretory production of hGM-CSF with a high specific biological activity by transgenic plant cell suspension culture. Biotech. Bioproc. Engin. 8: 135-141
9. Lee JS, Choi SJ, Kang, HS, Oh WG, Cho KH, Kwon TH, Kim DH, Jang YS, Yang MS (1997) Establishment of the transgenic tobacco cell suspension culture system for producing murine granulocyte-macrophage colony-stimulating factor. Mol. Cells 7: 783-787.
10. Libby RT, Braedt G, Kronheim SR, March CJ, Urdal DL, Chiaverotti TA, Tushinski RJ, Mochizuki DY, Hopp TP, Cosman D (1987) Expression and purification of native human granulocyte-macrophage colony-stimulating factor from an Escherichia coli secretion vector. DNA 6: 221-229.
11. Miele L (1997) Plants as bioreactors for biopharmaceuticals: regulatory considerations. Trends Biotechnol. 15: 45-50.
12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497.
13. Price V, Mochizuki D, March CJ, Cosman D, Deeley MC, Klinke R, Clevenger W, Gillis S, Baker P, Urdal D (1987) Expression, purification and characterization of recombinant murine granulocyte-macrophage colony-stimulating factor and bovine interleukin-2 from yeast. Gene 55: 287-293.
14. Rasko JE, Gough NM (1994) Granulocyte-macrophage colony-stimulating factor. In: Thomson A, ed. The Cytokine Handbook. London: Academic Press, pp. 343-369.
15. Su WW (1995) Bioprocessing technology for plant cell suspension cultures. Appl. Biochem. Biotech. 50: 189-230.
16. Yeoman MM, Yeoman CL (1996) Manipulating secondary metabolism in cultured plant cells. New Phytol. 134: 553-569.