Production and characterization of biologically active human GM-CSF secreted by genetically modified plant cells

Protein Expression and Purification

Volumn 19, Issue 1, 2000, Pages 131-138

  James, Eddie A ^a   Wang, Changlin ^b   Wang, Zeping ^b   Reeves, Raymond ^b   Shin, Joong Han ^a   Magnuson, Nancy S ^b   Lee, James M ^a  

^a Washington State University   (United States)

^b WASHINGTON STATE UNIVERSITY   (United States)

Author keywords

6 His tag; GM CSF; Plant cells; Protein stabilization; Suspension culture; Tobacco

Indexed keywords

AMINO TERMINAL SEQUENCE; CELL SECRETION; COMPLEMENTARY DNA; CULTURE MEDIUM; EXPRESSION VECTOR; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; MOLECULAR WEIGHT; PLANT CELL CULTURE; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SALT; SUSPENSION CELL CULTURE; TOBACCO; TOBACCO ETCH VIRUS; TRANSGENE; TRANSGENIC PLANT;

NICOTIANA TABACUM; TOBACCO ETCH VIRUS;

EID: 0034041013     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1232     Document Type: Article

References (25)

1. Hiatt A., Cafferkey R., Bowdish K. Production of antibodies in transgenic plants. Nature. 342:1989;76-78.
2. De Neve M., De Loose M., Jacobs A., Van Houdt H., Kaluza B., Weidle U., Van Montagu M., Depicker A. Assembly of an antibody and its derived antibody fragment in Nicotiana and Arabodipsis. Transgenic Res. 2:1993;227-237.
3. Magnuson N., Limzmaier P. M., Gao J., Reeves R., An G., Lee J. M. Enhanced recovery of a secreted mammalian protein from suspension culture of genetically modified tobacco cells. Protein Express. Purif. 7:1996;220-228.
4. Wongsamuth R., Doran P. M. Production of monoclonal antibodies by tobacco hairy roots. Biotechnol. Bioeng. 54:1997;401-415.
5. Firek S., Draper J., Owen M. R. L., Gandecha A., Cockburn B., Whitelam G. C. Secretion of a functional single-chain Fv protein in transgenic tobacco plants and cell suspension cultures. Plant Mol. Biol. 23:1993;861-870.
6. Conrad U., Fiedler R. Expression of engineered antibodies in plant cells. Plant Mol. Biol. 26:1994;1023-1030.
7. Sijmons P. C., Dekker B. M., Schrammeijer B., Verwoerd T. C., van den Elzen P. J. M., Hoekema A. Production of correctly processed human serum albumin in transgenic plants. Bio/Technology. 8:1990;217-221.
8. Trudel J., Potvin C., Asselin A. Secreted hen lysozyme in transgenic tobacco: Recovery of bound enzyme and in vitro growth inhibition of plant pathogens. Plant Sci. 106:1995;55-62.
9. Matsumoto S., Ikura K., Ueda M., Sasaki R. Characterization of a human glycoprotein (erythropoietin) produced in cultured tobacco cells. Plant Mol. Biol. 27:1995;1163-1172.
10. Magnuson N., Linzmaier P. M., Reeves R., An G., HayGlass K., Lee J. M. Secretion of biologically active human interleukin-2 and interleukin-4 from genetically modified tobacco cells in suspension culture. Protein Express. Purif. 13:1998;45-52.
11. Lee J., Choi S., Kang H., Oh W., Cho K., Kim D., Jang Y., Yang M. Establishment of a transgenic tobacco cell suspension for producing murine granulocyte-macrophage colony stimulating factor. Molecules Cells. 7:1997;783-787.
12. Kusnadi A. R., Nikolov Z. L., Howard J. A. Production of recombinant proteins in transgenic plants: Practical considerations. Biotechnol. Bioeng. 56:1997;473-484.
13. Düring K., Hippe S., Kreuzaler F., Schell J. Synthesis and self-assembly of a functional monoclonal antibody in transgenic Nicotiana tabacum. Plant Mol. Biol. 15:1990;281-293.
14. Gallie D. R., Tanguay L., Leathers V. The tobacco etch viral 5′ leader and poly(A) tail are functionally synergistic regulators of translation. Gene. 165:1995;233-238.
15. Allen G., Hall G., Michalowski S., Newman W., Spiker S., Weissinger A., Thompson W. High-level transgene expression in plant cells. Plant Cell. 8:1996;899-913.
16. Lund P., Dunsmuir P. A plant signal sequence enhances the secretion of bacterial ChiA in transgenic tobacco. Plant Mol. Biol. 18:1992;47-53.
17. Ma J. K.-C., Hiatt A., Hein M., Vine N. D., Wang F., Stabila P., van Dolleweerd C., Mostov K., Lehner T. Generation and assembly of secretory antibodies in plants. Science. 268:1995;716-719.
18. Wahl M. F., An G., Lee J. M. Effects of dimethyl sulfoxide on heavy chain monoclonal antibody production from plant cell culture. Biotechnol. Lett. 17:1995;463-468.
19. LaCount W., An G., Lee J. M. The effect of polyvinylpyrrolidone (PVP) on the heavy chain monoclonal antibody production from plant suspension cultures. Biotechnol. Lett. 11:1997;93-96.
20. Lee F., Yokota T., Otsuka T., Gemmell L., Larson N., Luh J., Arai K., Rennick D. Isolation of cDNA for a human granulocyte-macrophage colony-stimulating factor by functional expression in mammalian cells. Proc. Natl. Acad. Sci. USA. 82:1985;4360-4364.
21. Carrington J. C., Freed D. D. Cap-independent enhancement of translation by a plant potyvirus 5′ nontranslated region. J. Virol. 64:1990;1590-1597.
22. Murashige T., Skoog F. A revised medium for rapid growth and bio assay with tobacco tissue cultures. Plant Physiol. 15:1962;473-497.
23. Coligan J. E., Kruisbeek A. M., Margulies D., Shevach E., Stober W. Current Protocols in Immunology. 1990;Wiley, New York.
24. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London). 227:1970;680-683.
25. Voet D., Voet J. G. Biochemistry. 1995;Wiley, New York. p. 271-273.