Creating random mutagenesis libraries using megaprimer PCR of whole plasmid

BioTechniques

Volumn 33, Issue 5, 2002, Pages 1033-1038

  Miyazaki, Kentaro ^a   Takenouchi, Misa ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CLONING; MUTAGENESIS; SCREENING;

OLIGONUCLEOTIDE PRIMERS;

DNA;

DNA FRAGMENT; DNA POLYMERASE; GREEN FLUORESCENT PROTEIN; DNA DIRECTED DNA POLYMERASE; PFU DNA POLYMERASE; PHOTOPROTEIN; PRIMER DNA; RECOMBINANT DNA; TAQ POLYMERASE;

ARTICLE; DNA LIBRARY; GENE MUTATION; MOLECULAR CLONING; MUTAGENESIS; PLASMID; POLYMERASE CHAIN REACTION; SCREENING; SITE DIRECTED MUTAGENESIS; DNA TEMPLATE; EVALUATION; GENE LIBRARY; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; RANDOMIZATION; REPORTER GENE;

CLONING, MOLECULAR; DNA PRIMERS; DNA, RECOMBINANT; DNA-DIRECTED DNA POLYMERASE; GENE LIBRARY; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MUTAGENESIS; POLYMERASE CHAIN REACTION; RANDOM ALLOCATION; TAQ POLYMERASE; TEMPLATES, GENETIC;

EID: 1842842876     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/02335st03     Document Type: Article

References (9)

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