In vitro evolution of beta-glucuronidase into a beta-galactosidase proceeds through non-specific intermediates

Journal of Molecular Biology

Volumn 305, Issue 2, 2001, Pages 331-339

  Matsumura, Ichiro ^a   Ellington, Andrew D ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

Beta galactosidase; Beta glucuronidase; DNA shuffling; In vitro evolution; Substrate specificity

Indexed keywords

BACTERIAL DNA; BETA GALACTOSIDASE; BETA GLUCURONIDASE; GENE PRODUCT; PROTEIN GUSA; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SPECIFICITY; ESCHERICHIA COLI; EVOLUTION; GENE MUTATION; HYDROLYSIS; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL;

ESCHERICHIA COLI;

EID: 0035846958     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4259     Document Type: Article

References (39)

1. Directed evolution of new catalytic activity using the alpha/beta-barrel scaffold
2. Randomization of genes by PCR mutagenesis
3. Dissecting the catalytic triad of a serine protease
4. Redesigning secondary structure to invert coenzyme specificity in isopropylmalate dehydrogenase
5. DNA shuffling of a family of genes from diverse species accelerates directed evolution
6. Complete sequence and organization of the murine beta-glucuronidase gene
7. Guided evolution of enzymes with new substrate specificities
8. Selection of arnidases with novel substrate specificities from penicillin amidase of Escherichia coli
9. Random mutagenesis of the substrate-binding site of a serine protease can generate enzymes with increased activities and altered primary specificities
10. Experimental evolution of Ebg enzyme provides clues about the evolution of catalysis and to evolutionary potential
11. Engineering enzyme specificity
12. Converting trypsin to chymotrypsin: The role of surface loops
13. A classification of glycosyl hydrolases based on amino acid sequence similarities
14. Active site residues of human beta-glucuronidase. Evidence for Glu(540) as the nucleophile and Glu(451) as the acid-base residue
15. Three-dimensional structure of betagalactosidase from E. coli
16. Structure of human beta-glucuronidase reveals candidate lysosomal targeting and active-site motifs
17. Enzyme recruitment in evolution of new function
18. Laboratory evolution of peroxide-mediated cytochrome P450 hydroxylation
19. Directed evolution of the surface chemistry of the reporter enzyme beta-glucuronidase
20. Inverting enantioselectivity by directed evolution of hydantoinase for improved production of L-methionine
21. Exploring nonnatural evolutionary pathways by saturation mutagenesis: Rapid improvement of protein function
22. Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvent
23. Nucleotide sequence of rat preputial gland beta-glucuronidase cDNA and in vitro insertion of its encoded polypeptide into microsomal membranes
24. Redesign of the substrate specificity of Escherichia coli aspartate aminotransferase to that of Escherichia coli tyrosine aminotransferase by homology modeling and sitedirected mutagenesis
25. Cloning, sequencing, and expression of cDNA for human beta-glucuronidase
26. Redesigning the substrate specificity of an enzyme by cumulative effects of the mutations of non-active site residues
27. Identification of amino acid substitutions that alter the substrate specificity of TEM-1 beta-lactamase
28. Cloning of the canine beta-glucuronidase cDNA, mutation identification in canine MPS VII, and retroviral vector-mediated correction of MPS VII cells
29. A screen for SVgpt in E. coli DH5 delta lac and DH5 alpha: Small colony phenotype
30. Nucleotide sequence corrections of the uidA open reading frame encoding beta-glucuronidase
31. DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution
32. Rapid evolution of a protein in vitro by DNA shuffling
33. Some aspects of the evolution of metabolic pathways
34. Directed evolution of an aspartate arninotransferase with new substrate specificities
35. On earlier states of the biochemical system
36. The effect of highfrequency random mutagenesis on in vitro protein evolution: A study on TEM-1 beta-lactamase
37. An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues
38. Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening