Directed evolution of a bacterial α-amylase: Toward enhanced pH-performance and higher specific activity

Protein Science

Volumn 12, Issue 10, 2003, Pages 2141-2149

  Bessler, Cornelius ^a   Schmitt, Jutta ^b   Maurer, Karl Heinz ^a   Schmid, Rolf D ^b  

^a HENKEL KGAA   (Germany)

^b UNIVERSITY OF STUTTGART   (Germany)

Author keywords

amylase; Directed evolution; High throughput assay; pH activity profile; Specific activity

Indexed keywords

AMYLASE; DETERGENT; STARCH;

ALKALINITY; ARTICLE; BACILLUS AMYLOLIQUEFACIENS; CONTROLLED STUDY; CYTOPLASM; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SPECIFICITY; EXTRACTION; GENE MUTATION; NONHUMAN; PH; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RELIABILITY;

ALPHA-AMYLASE; AMINO ACID SEQUENCE; BACILLUS; DIRECTED MOLECULAR EVOLUTION; DNA MUTATIONAL ANALYSIS; DNA SHUFFLING; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TRANSFORMATION, BACTERIAL;

BACILLUS AMYLOLIQUEFACIENS; BACTERIA (MICROORGANISMS);

EID: 0141858719     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.0384403     Document Type: Article

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