An effective family shuffling method using single-stranded DNA

Gene

Volumn 243, Issue 1-2, 2000, Pages 133-137

  Kikuchi, Miho ^a   Ohnishi, Kouhei ^a   Harayama, Shigeaki ^a  

^a MARINE BIOTECHNOLOGY INSTITUTE   (Japan)

Author keywords

Family shuffling; nahH; Protein evolution; Single stranded DNA; xylE

Indexed keywords

CATECHOL 2,3 DIOXYGENASE; CHIMERIC PROTEIN; DEOXYRIBONUCLEASE; RESTRICTION ENDONUCLEASE; SINGLE STRANDED DNA;

ARTICLE; ESCHERICHIA COLI; EVOLUTION; GENE SEQUENCE; GENE STRUCTURE; MULTIGENE FAMILY; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN PROCESSING;

CATECHOL 2,3-DIOXYGENASE; DIOXYGENASES; DNA; DNA, SINGLE-STRANDED; ENZYME STABILITY; GENETIC ENGINEERING; OXYGENASES; RECOMBINANT FUSION PROTEINS; TEMPERATURE;

EID: 0033964975     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(99)00547-8     Document Type: Article

References (17)

1. Chang C.-C., Chen T.T., Cox B.W., Dawes G.N., Stemmer W.P., Punnonen J., Patten P.A. Evolution of a cytokine using DNA family shuffling. Nat. Biotechnol. 17:1999;793-797.
2. Crameri A., Whitehorn E.A., Tate E., Stemmer W.P. Improved green fluorescent protein by molecular evolution using DNA shuffling. Nat. Biotechnol. 14:1996;315-319.
3. Crameri A., Dawes G., Rodriguez E. Jr., Silver S., Stemmer W.P. Molecular evolution of an arsenate detoxification pathway by DNA shuffling. Nat. Biotechnol. 15:1997;436-438.
4. Crameri A., Raillard S.A., Bermudez E., Stemmer W.P. DNA shuffling of a family of genes from diverse species accelerates directed evolution. Nature. 391:1998;288-291.
5. Hansson L.O., Bolton-Grob R., Massoud T., Mannervik B. Evolution of differential substrate specificities in Mu class glutathione transferases probed by DNA shuffling. J. Mol. Biol. 287:1999;265-276.
6. Harayama S. Artificial evolution by DNA shuffling. Trends Biotechnol. 16:1998;76-82.
7. Kikuchi M., Ohnishi K., Harayama S. Novel family shuffling methods for the in vitro evolution of enzymes. Gene. 236:1999;159-167.
8. Kumamaru T., Suenaga H., Mitsuoka M., Watanabe T., Furukawa K. Enhanced degradation of polychlorinated biphenyls by directed evolution of biphenyl dioxygenase. Nat. Biotechnol. 16:1998;663-666.
9. Melgar E., Goldthwait D.A. Deoxyribonucleic acid nucleases. II. The effects of metals on the mechanism of action of deoxyribonuclease I. J. Biol. Chem. 243:1968;4409-4418.
10. Moore J.C., Jin H.M., Kuchner O., Arnold F.H. Strategies for the in vitro evolution of protein function: enzyme evolution by random recombination of improved sequences. J. Mol. Biol. 272:1997;336-347.
11. Patten P.A., Howard R.J., Stemmer W.P. Applications of DNA shuffling to pharmaceuticals and vaccines. Curr. Opin. Biotechnol. 8:1997;724-733.
12. Stemmer W.P. DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution. Proc. Natl. Acad. Sci. USA. 91:1994;10747-10751.
13. Stemmer W.P. Rapid evolution of a protein in vitro by DNA shuffling. Nature. 370:1994;389-391.
14. Vieira J., Messing J. Production of single-stranded plasmid DNA. Meth. Enzymol. 153:1987;3-11.
15. Yano T., Oue S., Kagamiyama H. Directed evolution of an aspartate aminotransferase with new substrate specificities. Proc. Natl. Acad. Sci. USA. 95:1998;5511-5515.
16. Zhang J.H., Dawes G., Stemmer W.P. Directed evolution of a fucosidase from a galactosidase by DNA shuffling and screening. Proc. Natl. Acad. Sci. USA. 94:1997;4504-4509.
17. Zhao H., Arnold F.H. Optimization of DNA shuffling for high fidelity recombination. Nucleic Acids Res. 25:1997;1307-1308.