Degenerate oligonucleotide gene shuffling (DOGS): A method for enhancing the frequency of recombination with family shuffling

Gene

Volumn 271, Issue 1, 2001, Pages 13-20

  Gibbs, Moreland D ^a   Nevalainen, K M Helena ^a   Bergquist, Peter L ^a,b  

^a MACQUARIE UNIVERSITY   (Australia)

^b UNIVERSITY OF AUCKLAND   (New Zealand)

Author keywords

Gene shuffling; Overlap extension PCR; Xylanase genes

Indexed keywords

ENDONUCLEASE; GENE PRODUCT; OLIGONUCLEOTIDE; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; CHIMERA; DEGENERATE OLIGONUCLEOTIDE GENE SHUFFLING; ENZYME ACTIVITY; GENE FREQUENCY; GENE ISOLATION; GENE MUTATION; GENE REARRANGEMENT; GENETIC CODE; GENETIC RECOMBINATION; MOLECULE; MULTIGENE FAMILY; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; TECHNIQUE;

AMINO ACID SEQUENCE; BACTERIA, ANAEROBIC; BASE SEQUENCE; BETA-GLUCOSIDASE; DNA PRIMERS; ENDO-1,4-BETA XYLANASES; GENE REARRANGEMENT; HYDROGEN-ION CONCENTRATION; METHODS; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDES; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; SEQUENCE HOMOLOGY, AMINO ACID; SEQUENCE HOMOLOGY, NUCLEIC ACID; TEMPERATURE; XYLOSIDASES;

EID: 0035854021     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(01)00506-6     Document Type: Article

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