DNA-dependent phosphorylation of Chk1 and Claspin in a human cell-free system

Biochemical Journal

Volumn 388, Issue 2, 2005, Pages 705-712

  Clarke, Catriona A L ^a   Clarke, Paul R ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

Cell cycle; Checkpoint; Chk1; Claspin; DNA damage; Protein kinase

Indexed keywords

BIOCHEMISTRY; DNA; GENES; GENETIC ENGINEERING; PROTEINS;

CLASPIN; DNA DAMAGE; OLIGONUCLEOTIDES; PHOSPHORYLATION;

CELLS;

ATR PROTEIN; BINDING PROTEIN; CHECKPOINT KINASE 1; OLIGONUCLEOTIDE; PROTEIN CLASPIN; SERINE; THREONINE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CELL CYCLE; CELL FREE SYSTEM; CELL FUNCTION; CHEMICAL ANALYSIS; CONTROLLED STUDY; DNA BINDING; ENZYME ACTIVATION; ENZYME ACTIVITY; HUMAN; HUMAN CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN INTERACTION; PROTEIN ISOLATION; PROTEIN MOTIF; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION;

ADAPTOR PROTEINS, SIGNAL TRANSDUCING; AMINO ACID SEQUENCE; CELL-FREE SYSTEM; DNA; HUMANS; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDES; PHOSPHOPEPTIDES; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN KINASE INHIBITORS; PROTEIN KINASES; SEQUENCE HOMOLOGY, AMINO ACID;

ATAXIA TELANGIECTASIA;

EID: 20544478438     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20041966     Document Type: Article

References (44)

1. O'Connell, M. J., Walworth, N. C. and Carr, A. M. (2000) The G2-phase DNA-damage checkpoint. Trends Cell Biol. 10, 296-303
2. Melo, J. and Toczyski, D. (2002) A unified view of the DNA-damage checkpoint. Curr. Opin. Cell Biol. 14, 237-245
3. Walworth, N., Davey, S. and Beach, D. (1993) Fission yeast chk1 protein kinase links the rad checkpoint pathway to cdc2. Nature (London) 363, 368-371
4. Furnari, B., Rhind, N, and Russell, P. (1997) Cdc25 mitotic inducer targeted by chk1 DNA damage checkpoint kinase. Science 277, 1495-1497
5. Peng, C. Y., Graves, P. R., Thoma, R. S., Wu, Z., Shaw, A. S. and Piwnica-Worms, H. (1997) Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216. Science 277, 1501-1505
6. Sanchez, Y., Wong, C., Thoma, R. S., Richman, R., Wu, Z., Piwnica-Worms, H. and Elledge, S. J. (1997) Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25. Science 277, 1497-1501
7. Zeng, Y., Forbes, K. C., Wu, Z., Moreno, S., Piwnica-Worms, H. and Enoch, T (1998) Replication checkpoint requires phosphorylation of the phosphatase Cdc25 by Cds1 or Chk1. Nature (London) 395, 507-510
8. Kumagai, A., Quo, Z., Emami, K. H., Wang, S. X. and Dunphy, W. G. (1998) The Xenopus Chk1 protein kinase mediates a caffeine-sensitive pathway of checkpoint control in cell-free extracts. J. Cell Biol. 142, 1559-1569
9. Blasina, A., de Weyer, I.V., Laus, M. C., Luyten, W. H., Parker, A. E, and McGowan, C. H. (1999) A human homologue of the Checkpoint kinase Cds1 directly inhibits Cdc25 phosphatase. Curr. Biol. 9, 1-10
10. Furnari, B., Blasina, A., Boddy, M. N., McGowan, C. H. and Russell, P. (1999) Cdc25 inhibited in vivo and in vitro by checkpoint kinases Cds1 and Chk1. Mol. Biol. Cell 10, 833-845
11. O'Connell, M. J., Raleigh, J. M., Verkade, H. M. and Nurse, P (1997) Chk1 is a wee1 kinase in the G2 DNA damage Checkpoint inhibiting cdc2 by Y15 phosphorylation. EMBO J. 16, 545-554
12. Lee, J., Kumagai, A. and Dunphy, W. G. (2001) Positive regulation of Wee1 by Chk1 and 14-3-3 proteins. Mol. Biol. Cell 12, 551-563
13. Russell, P. (1998) Checkpoints on the road to mitosis. Trends Biochem. Sci. 23, 399-402
14. Mailand, N., Falck, J., Lukas, C., Syljuasen, R. G., Welcker, M., Bartek, J, and Lukas, J. (2000) Rapid destruction of human Cdc25A in response to DNA damage. Science 288, 1425-1429
15. Sorensen, C, S., Syljuasen, R. G., Falck, J., Schroeder, T., Ronnstrand, L., Khanna, K. K., Zhou, B. B., Bartek, J. and Lukas, J. (2003) Chk1 regulates the S phase checkpoint by coupling the physiological turnover and ionizing radiation-induced accelerated proteolysis of Cdc25A. Cancer Cell 3, 247-258
16. Liu, Q., Guntuku, S., Cui, X. S., Matsuoka, S., Cortez, D., Tamai, K., Luo, G., Carattini-Rivera, S., DeMayo, F., Bradley, A. et al. (2000) Chk1 is an essential kinase that is regulated by ATR and required for the G(2)/M DNA damage checkpoint. Genes Dev. 14, 1448-1459
17. Lopez-Girona, A., Tanaka, K., Chen, X, B., Baber, B. A., McGowan, C. H. and Russell, P. (2001) Serine-345 is required for Rad3-dependent phosphorylation and function of checkpoint kinase Chk1 in fission yeast. Proc. Natl. Acad. Sci. U.S.A. 98, 11289-11294
18. Zhao, H. and Piwnica-Worms, H. (2001) ATR-mediated checkpoint pathways regulate phosphorylation and activation of human Chk1. Mol. Cell. Biol. 21, 4129-4139
19. Jiang, K., Pereira, E., Maxfield, M., Russell, B., Goudelock, D. M. and Sanchez, Y. (2003) Regulation of Chk1 includes chromatin association and 14-3-3 binding following phosphorylation on Ser-345, J. Biol. Chem. 278, 25207-25217
20. Gatei, M., Sloper, K., Sorensen, C., Syljuasen, R., Falck, J., Hobson, K., Savage, K., Lukas, J., Zhou, B. B., Bartek, J. et al. (2003) Ataxia-telangiectasia-mutated (ATM) and NBS1-dependent phosphorylation of Chk1 on Ser-317 in response to ionizing radiation J. Biol. Chem. 278, 14806-14811
21. Walworth, N. C. and Bernards, R. (1996) rad-dependent response of the chk1-encoded protein kinase at the DNA damage checkpoint. Science 271, 353-356
22. Takai, H., Tominaga, K., Motoyama, N., Minamishima, Y. A., Nagahama, H., Tsukiyama, T., Ikeda, K., Nakayama, K., Nakanishi, M. and Nakayama, K. I. (2000) Aberrant cell cycle checkpoint function and early embryonic death in Chk1 (-/-) mice. Genes Dev. 14, 1439-1447
23. Zachos, G., Rainey, M. D. and Gillespie, D. A. (2003) Chk1-deficient tumour cells are viable but exhibit multiple checkpoint and survival defects. EMBO J. 22, 713-723
24. Lam, M. H., Liu, Q., Elledge, S. J, and Rosen, J. M. (2004) Chk1 is haploinsufficient for multiple functions critical to tumor suppression. Cancer Cell 6, 45-59
25. Zhou, B. B., Anderson, H. J. and Roberge, M. (2003) Targeting DNA checkpoint kinases in cancer therapy. Cancer Biol. Ther. 2, S16-S22
26. Dasso, M. and Newport, J. W. (1990) Completion of DNA replication is monitored by a feedback system that controls the initiation of mitosis in vitro: studies in Xenopus. Cell (Cambridge, Mass.) 61, 811-823
27. Kornbluth, S., Smythe, C. and Newport, J. W. (1992) In vitro cell cycle arrest induced by using artificial DNA templates. Mol. Cell Biol. 12, 3216-3223
28. Kumagai, A. and Dunphy, W. G. (2000) Claspin, a novel protein required for the activation of Chk1 during a DNA replication checkpoint response in Xenopus egg extracts. Mol. Cell 6, 839-849
29. Quo, Z., Kumagai, A., Wang, S. X. and Dunphy, W. G. (2000) Requirement for ATR in phosphorylation of Chk1 and cell cycle regulation in response to DNA replication blocks and UV-damaged DNA in Xenopus egg extracts. Genes Dev. 14, 2745-2756
30. Hekmat-Nejad, M., You, Z., Yee, M. C., Newport, J. W. and Cimprich, K. A. (2000) Xenopus ATR is a replication-dependent chromatin-binding protein required for the DNA replication checkpoint. Curr. Biol. 10, 1565-1573
31. Kumagai, A., Kim, S. M. and Dunphy, W. G. (2004) Claspin and the activated form of ATR-ATRIP collaborate in the activation of Chk1. J. Biol. Chem. 279, 49599-49608
32. Lee, J., Kumagai, A. and Dunphy, W. G. (2003) Claspin, a Chk1-regulatory protein, monitors DNA replication on chromatin independently of RPA, ATR, and Rad17. Mol. Cell 11, 329-340
33. Kumagai, A, and Dunphy, W. G. (2003) Repeated phosphopeptide motifs in Claspin mediate the regulated binding of Chk1. Nat. Cell Biol. 5, 161-165
34. Jeong, S. Y., Kumagai, A., Lee, J. and Dunphy, W. G. (2003) Phosphorylated Claspin interacts with a phosphate-binding site in the kinase domain of Chk1 during ATR-mediated activation. J. Biol. Chem. 278, 46782-46788
35. Chini, C. C. and Chen, J. (2003) Human Claspin is required for replication checkpoint control. J. Biol. Chem. 278, 30057-30062
36. Lin, S. Y., Li, K., Stewart, G. S. and Elledge, S. J. (2004) Human Claspin works with BRCA1 to both positively and negatively regulate cell proliferation. Proc. Natl. Acad. Sci. U.S.A. 101, 6484-6489.
37. Hutchins, J. R. A., Hughes, M. and Clarke, P. R. (2000) Substrate specificity determinants of the checkpoint protein kinase Chk1. FEBS Lett. 466, 91-95
38. Dignam, J. D., Lebovitz, R. M. and Roeder, R. G. (1983) Accurate transcription initiation by RNA ploymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 11, 1475-1489
39. Quo, Z. and Dunphy, W. G. (2000) Response of Xenopus Cds1 in cell-free extracts to DNA templates with double-stranded ends. Mol. Biol. Cell 11, 1535-1546
40. Palermo, C. and Walworth, N. C. (2005) Assaying cell cycle checkpoints: activity of the protein kinase Chk1. Methods Mol. Biol. 296, 345-354
41. Graves, P. R., Yu, L., Schwarz, J. K., Gales, J., Sausville, E A., O'Connor, P. M. and Piwnica-Worms, H. (2000) The Chk1 protein kinase and the Cdc25C regulatory pathways are targets of the anticancer agent UCN-01. J. Biol. Chem. 275, 5600-5605
42. Busby, E. C., Leistritz, D. F., Abraham, R. T., Karnitz, L. M. and Sarkaria, J. N. (2000) The radiosensitizing agent 7-hydroxystaurosporine (UCN-01) inhibits the DNA damage checkpoint kinase hChk1 Cancer Res. 60, 2108-2112
43. Davies, S. P., Reddy, H., Caivano, M. and Cohen, P. (2000) Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem. J. 351, 95-105
44. Yoo, H. Y., Kumagai, A., Shevchenko, A. and Dunphy, W. G. (2004) Adaptation of a DNA replication checkpoint response depends upon inactivation of Claspin by the Polo-like kinase. Cell (Cambridge, Mass.) 117, 575-588