Commercial production of β-glucuronidase (GUS): A model system for the production of proteins in plants

Molecular Breeding

Volumn 4, Issue 4, 1998, Pages 301-312

  Witcher, Derrick R ^a   Hood, Elizabeth E ^b   Peterson, Dave ^a   Bailey, Michele ^b   Bond, Diane ^a   Kusnadi, Ann ^c   Evangelista, Roque ^c   Nikolov, Zivko ^c   Wooge, Cynthia ^d   Mehigh, Richard ^d   Kappel, William ^d   Register, James ^a   Howard, John A ^b  

^a PIONEER HI BRED INTERNATIONAL INC   (United States)

^b Prodigene Inc   (United States)

^c IOWA STATE UNIVERSITY   (United States)

^d Sigma Chemical Company   (United States)

Author keywords

Heterologous gene; Localization; Processing; Protein production; Protein purification; Seed expression; Transgenic maize; glucuronidase (GUS)

Indexed keywords

AMINO ACID SEQUENCE; BETA GLUCURONIDASE; BIOREACTOR; CYTOPLASM; GENE EXPRESSION; HYMECROMONE GLUCURONIDE; ISOELECTRIC POINT; METHIONINE; PLANT GENETICS; PLANT MODEL; PLANT PRODUCT; PROTEIN PURIFICATION; RECOMBINANT ENZYME; SACCHAROLACTONE; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS; SOUTHERN BLOTTING; TRANSGENIC PLANT;

AMINO ACIDS; CLONING; CYTOLOGY; ELECTROPHORESIS; ENZYMES; ESCHERICHIA COLI; GENE EXPRESSION; PLANTS (BOTANY); PURIFICATION; SEED;

COMMERCIAL PRODUCTIONS; GLUCURONIDASE; HETEROLOGOUS GENES; LOCALISATION; PROCESSING; PROTEIN PRODUCTION; PROTEIN PURIFICATION; SEED EXPRESSION; TRANSGENIC MAIZE; Β-GLUCURONIDASE;

RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 0011977387     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1009622429758     Document Type: Article

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