Accumulation of a thermostable endo-1,4-β-D-glucanase in the apoplast of Arabidopsis thaliana leaves

Molecular Breeding

Volumn 6, Issue 1, 2000, Pages 37-46

  Ziegler, Matthew T ^a   Thomas, Steven R ^b   Danna, Kathleen J ^a  

^a UNIVERSITY OF COLORADO   (United States)

^b NATIONAL RENEWABLE ENERGY LABORATORY   (United States)

Author keywords

Apoplast; Biomass conversion; Thermostable endo 1,4 D glucanase; Transgenic Arabidopsis thaliana

Indexed keywords

ACIDOTHERMUS CELLULOLYTICUS; APOPLAST; BIOACCUMULATION; BIOMASS CONVERSION; CELLULASE; CELLULOSE; ENZYME RELEASE; ENZYME SYNTHESIS; ENZYMIC HYDROLYSIS; GENE TARGETING; PROTEIN TARGETING; SIGNAL PEPTIDE; SUSPENSION CELL CULTURE; THERMOSTABILITY; TRANSGENIC PLANT;

ALTERNATIVE FUELS; BIOCONVERSION; BIOMASS; ENZYMATIC HYDROLYSIS; PLANTS (BOTANY); TOBACCO; VIRUSES;

APOPLASTS; ARABIDOPSIS THALIANA; BIOMASS CONVERSION; CATALYTIC DOMAINS; GLUCANASE; PLANT BIOMASS; SIGNAL PEPTIDE; THERMOSTABLE ENDO-1,4-Β-D-GLUCANASE; TRANSGENIC ARABIDOPSI THALIANUM; TRANSGENIC ARABIDOPSIS;

PEPTIDES;

ACIDOTHERMUS CELLULOLYTICUS; ARABIDOPSIS THALIANA; BRASSICA OLERACEA VAR. BOTRYTIS; CAULIFLOWER MOSAIC VIRUS; NICOTIANA TABACUM; TOBACCO MOSAIC VIRUS;

EID: 0033996431     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1009667524690     Document Type: Article

References (41)

1. An G: High efficiency transformation of cultured tobacco cells. Plant Physiol 79: 568-570 (1985).
2. An G, Ebert R, Mitra A, Ha SB: Binary vectors. In: Gelvin SB, Schilperoort RA, Verma DPS (eds) Plant Molecular Biology Manual, pp. A3/1-A3/19. Kluwer Academic Publishers, Dordrecht, Netherlands (1988).
3. Aspegren K, Mannonen I, Ritala A, Teeri TH: Production of fungal, heat-stable β-glucanase in suspension cultures of transgenic barley cells. In: Owen, MRL, Pen, J (eds) Transgenic Plants: A Production System for Industrial and Pharmaceutical Proteins, pp. 201-212 John Wiley, London (1996).
4. 5. Appl Biochem Biotech 45/46: 245-256 (1994).
5. Baker JO, Adney WS, Thomas SR, Nieves RA, Chou Y-C, Vinzant TB, Tucker MP, Laymon RA, Himmel ME: Synergism between purified bacterial and fungal cellulases. In: Saddler JN, Penner MH (eds) Enzymatic Degradation of Insoluble Carbohydrates, ACS Symposium Series 618, pp. 113-141. American Chemical Society, Washington, DC (1995).
6. Benfey PN, Chua N-H: The cauliflower mosaic virus 35S promoter: combinatorial regulation of transcription in plants. Science 250: 959-966 (1990).
7. Bent AF, Clough SJ: Agrobacterium germ-line transformation: transformation of Arabidopsis without tissue culture. In: Gelvin SB, Schilperoort RA, Verma DPS (eds) Plant Molecular Biology Manual, pp. B7: 1-14. Kluwer Academic Publishers, Dordrecht, Netherlands (1998).
8. Bollag DM, Rozycki MD, Edelstein SJ: Protein Methods, 2nd ed. John Wiley, New York (1996).
9. Brett C, Waldron K: Physiology and Biochemistry of Plant Cell Walls, 2nd ed. Chapman & Hall, London (1996).
10. Chalfie MI, Tu Y, Euskirchen G, Ward WW, Prasher DC: Green fluorescent protein as a marker for gene expression. Science 263: 802-805 (1994).
11. Coughlan MP: Staining techniques for the detection of the individual components of cellulolytic enzyme systems. Meth Enzymol 160: 135-144 (1988).
12. Elander RT, Putsche VL: Ethanol from corn: technology and economics. In: Wyman CE (ed) Handbook on Bioethanol: Production and Utilization, pp. 329-349. Taylor and Francis, Washington, DC (1996).
13. Gallie DR, Sleat DE, Watts W, Turner PC, Wilson TMA: The 5′-leader sequence of tobacco mosaic virus RNA enhances the expression of foreign gene transcripts in vitro and in vivo. Nucl Acids Res 15: 3257-3273 (1987).
14. Hammond-Kosack KE, Harrison K, Jones JDG: Develomentally regulated cell death on expression of the fungal avirulence gene Avr9 in tomato seedlings carrying the disease-resistance gene Cf-9. Proc Natl Acad Sci USA 91: 10445-10449 (1994).
15. Haseloff J, Siemering KR, Prasher DC, Hodge S: Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly. Proc Natl Acad Sci USA 94: 2122-2127 (1997).
16. Hennegan KH, Danna KJ: pBIN20: an improved binary vector for Agrobacterium-mediated transformation. Plant Mol Biol Rep 16: 129-131 (1998).
17. Henrissat B, Bairoch A: New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem J 293: 781-788 (1993).
18. Herbers K, Wilke I, Sonnewald U: A thermostable xylanase from Clostridium thermocellum expressed at high levels in the apoplast of transgenic tobacco has no detrimental effects and is easily purified. Bio/technology 13: 63-66 (1995).
19. Hunt MD, Delaney TP, Dietrich RA, Weymann KB, Dangl JL, Ryals, JA: Salicylate-independent lesion formation in Arabidopsis lsd mutants. Mol Plant-Microbe Interact 10: 531-536 (1997).
20. Iglesias VA, Moscone EA, Papp I, Neuhuber F, Michalowski S, Phelan T, Spiker S, Matzke M, Matzke AJM: Molecular and cytogenetic analyses of stably and unstably expressed transgene loci in tobacco. Plant Cell 9: 1251-1264 (1997).
21. Jensen LG, Olsen O, Kops O, Wolf N, Thomsen KK, von Wettstein D: Transgenic barley expressing a protein-engineered, thermostable (1,3-1,4)- β-glucanase during germination. Proc Natl Acad Sci USA 93: 3487-3491 (1996).
22. Kadam K: Cellulase production. In: Wyman CE (ed) Handbook on Bioethanol: Production and Utilization, pp. 213-252. Taylor and Francis, Washington, DC (1996).
23. Kusnadi AR, Nikolov ZL, Howard JA: Production of recombinant proteins in transgenic plants: practical considerations. Biotechnol Bioeng 56: 473-484 (1997).
24. Luehrsen KR, de Wet JR, Walbot V: Transient expression analysis in plants using firefly luciferase reporter gene. Meth Enzymol 216: 397-414 (1992).
25. Lynd LR, Cushman JH, Nichols RJ, Wyman CE: Fuel ethanol from cellulosic biomass. Science 251: 1318-1323 (1991).
26. McBride KE, Schaaf DJ, Daley M, Stalker DM: Controlled expression of plastid transgenes in plants based on a nuclear DNA-encoded and plastid-targeted T7 RNA polymerase. Proc Natl Acad Sci USA 91: 7301-7305 (1994).
27. Mohagheghi A, Grohmann K, Himmel M, Leighton L, Updegraff DM: Isolation and characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a new genus of thermophilic, acidophilic, cellulolytic bacteria. Int J Syst Bact 36: 435-443 (1986).
28. 3 exoglucanase using enzyme-linked immunosorbent assay (ELISA). Appl Biochem Biotech 51/52: 211-223 (1995).
29. Owen MRL, Pen J (eds) Transgenic Plants: A Production System for Industrial and Pharmaceutical Proteins. John Wiley, London (1996).
30. Pelham HRB: The retention signal for soluble proteins of the endoplasmic reticulum. Trends Biochem Sci 15: 483-486 (1990).
31. Phillipson BA: Expression of a hybrid (1-3, 1-4)-β-glucanase in barley protoplasts. Plant Sci 91: 195-206 (1993).
32. Reiter W-D, Chapple CCS, Somerville CR: Altered growth and cell walls in a fucose-deficient mutant of Arabidopsis. Science 261: 1032-1035 (1993).
33. Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual, 2nd ed., p. A.1. Cold Spring Harbor Laboratory Press, Plainview, NY (1989).
34. Shaw, CH: Introduction of cloning plasmids into Agrobacterium tumefaciens. Meth Mol Biol 49: 33-37 (1995).
35. Shedletzky E, Shmuel M, Trainin T, Kalman, S, Delmer D: Cell wall structure in cells adapted to growth on the cellulose-synthesis inhibitor 2,6-dichlorobenzonitrile. Plant Physiol 100: 120-130 (1992).
36. Tomme P, Warren RAJ, Gilkes NR: Cellulose hydrolysis by bacteria and fungi. Adv Microb Physiol 37: 1-81 (1995).
37. Towner P, Purification of DNA. In: Brown A (ed) Essential Molecular Biology, vol. I, pp. 47-68. Oxford Univerity Press, Oxford (1991).
38. Tucker MP, Mohagheghi A, Grohmann, K, Himmel ME: Ultra-thermostable cellulases from Acidothermus cellulolyticus: comparison of temperature optima with previously reported cellulases. Bio/Technology 7: 17-820 (1989).
39. Turner SR, Somerville CR: Collapsed xylem phenotype of Arabidopsis identifies mutants deficient in cellulose deposition in the secondary cell wall. Plant Cell 9: 689-701 (1997).
40. Verwoerd TC, van Paridon PA, van Ooyen AJJ, van Lent JWM, Hoekema A, Pen J: Stable accumulation of Aspergilus niger phytase in transgenic tobacco leaves. Plant Physiol 109: 1199-1205 (1995).
41. Wood PJ, Erfle JD, Teather RM: Use of complex formation between Congo Red and polysaccharides in detection and assay of polysaccharide hydrolases. Meth Enzymol 160: 59-75 (1988).