A chloroplast transgenic approach to hyper-express and purify Human Serum Albumin, a protein highly susceptible to proteolytic degradation

Plant Biotechnology Journal

Volumn 1, Issue 2, 2003, Pages 71-79

  Fernández San Millán, Alicia ^a   Mingo Castel, Angel ^b   Miller, Michael ^c   Daniell, Henry ^a  

^a UNIVERSITY OF CENTRAL FLORIDA   (United States)

^b PUBLIC UNIVERSITY OF NAVARRE   (Spain)

^c AUBURN UNIVERSITY   (United States)

Author keywords

biopharmaceuticals; chloroplast genetic engineering; genetically modified crops; molecular farming; recombinant human blood proteins

Indexed keywords

EID: 85169106486     PISSN: 14677644     EISSN: 14677652     Source Type: Journal    
DOI: 10.1046/j.1467-7652.2003.00008.x     Document Type: Article

References (43)

1. Bogorad, L. (2000) Engineering chloroplasts: an alternative site for foreign genes, proteins, reactions and products. Trends Biotechnol. 18, 257–263.pmid/10802561
2. Carrio, M.M., Corchero, J.L. and Villaverde, A. (1999) Proteolytic digestion of bacterial inclusion body proteins during dynamic transition between soluble and insoluble forms. Biochim. Biophys. Act. 1434, 170–176.
3. Daniell, H. (1997) Transformation and foreign gene expression in plants mediated by microprojectile bombardment. Meth. Mol. Biol. 62, 463–489.
4. Daniell, H. (2002) Molecular strategies for gene containment in transgenic crops. Nat. Biotechnol. 20, 581–586.pmid/12042861
5. Daniell, H., Datta, R., Varma, S., Gray, S. and Lee, S.B. (1998) Containment of herbicide resistance through genetic engineering of the chloroplast genome. Nat. Biotechnol. 16, 345–348.pmid/9555724
6. Daniell, H. and Dhingra, A. (2002) Multigene engineering: Dawn of an exciting new era in biotechnology. Curr. Opin. Biotechnol. 13, 136–141.pmid/11950565
7. Daniell, H., Dhingra, A. and Fernandez-San Millan, A. (2001a) Chloroplast transgenic approach for the production of antibodies, biopharmaceuticals and edible vaccines. Proceedings of the International Congress on Photosynthesis, S40–04. Brisbane, Australia. Colling wood, Australia: CSIRO Publishing.
8. Daniell, H., Khan, M.S. and Allison, L. (2002) Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology. Trends Plant Sci. 7, 84–91.pmid/11832280
9. Daniell, H., Lee, S.B., Panchal, T. and Wiebe, P.O. (2001b) Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts. J. Mol. Biol. 311, 1001–1009.pmid/11531335
10. Daniell, H., Muthukumar, B. and Lee, S.B. (2001c) Marker free transgenic plants: Engineering the chloroplast genome wihout the use of antibiotic selection. Curr. Genet. 39, 109–116.pmid/11405095
11. Daniell, H., Streatfield, S. and Wycoff, K. (2001d) Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends Plant Sci. 6, 219–226.pmid/11335175
12. Daniell, H., Vivekananda, J., Neilsen, B., Ye, G.N., Tewari, K.K. and Sanford, J.C. (1990) Transient foreign gene expression in chloroplasts of cultured tobacco cells after biolistic delivery of chloroplast vectors. Proc. Natl Acad. Sci. USA, 87, 88–92.pmid/2404285
13. De Cosa, B., Moar, W., Lee, S.B., Miller, M. and Daniell, H. (2001) Overexpression of the Bt cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystals. Nat. Biotechnol. 19, 71–74.pmid/11135556
14. DeGray, G., Rajasekaran, K., Smith, F., Sanford, J. and Daniell, H. (2001) Expression of an antimicrobial peptide via the chloroplast genome to control phytopathogenic bacteria and fungi. Plant Physiol. 127, 852–862.pmid/11706168
15. Dodsworth, N., Harris, R., Denton, K., Woodrow, P., Wood, C. and Quirk, A. (1996) Comparative studies of recombinant human albumin and human serum albumin derived by blood fractionation. Biotechnol. Appl. Biochem. 24, 171–176.pmid/8865608
16. Eibl, C., Zou, Z., Beck, A., Kim, M., Mullet, J. and Koop, H.U. (1999) In vivo analysis of plastid psbA, rbcL and rpl32 UTR elements by chloroplast transformation: tobacco plastid gene expression is controlled by modulation of transcript levels and translation efficiency. Plant J. 19, 333–345.pmid/10476080
17. Enfors, S.O. (1992) Control of in vivo proteolysis in the production of recombinant proteins. Trends Biotechnol. 10, 310–315.pmid/1369412
18. Farran, I., Sanchez-Serrano, J.J., Medina, J.F., Prieto, J. and Mingo-Castel, A.M. (2002) Targeted expression of human serum albumin to potato tubers. Transgenic Res. 11, 337–346.pmid/12212837
19. Fleer, R., Yeh, P., Amellal, N., Maury, I., Fournier, A., Bacchetta, F., Baduel, P., Jung, G., Lhote, H., Becquart, J., Fukuhara, H. and Mayaux, J.F. (1991) Stable multicopy vectors for high-level secretion of recombinant human serum albumin by Kluyveromyces yeasts. Bio/Technol. 9, 968–975.
20. Guda, C., Lee, S.B. and Daniell, H. (2000) Stable expression of a biodegradable protein-based polymer in tobacco chloroplasts. Plant Cell Report, 19, 257–262.
21. Ketchner, S.L., Drapier, D., Olive, J., Gaudriault, S., Girard-Bascou, J. and Wollman, F.A. (1995) Chloroplasts can accomodate inclusion bodies. J. Biol. Chem. 270, 15299–15306.pmid/7797517
22. Kota, M., Daniell, H., Varma, S., Garczynski, F., Gould, F. and Moar, W.J. (1999) Overexpression of the Bacillus thuringiensis (Bt) Cry2Aa2 protein in chloroplasts confers resistance to plants against susceptible and Bt-resistant insects. Proc. Natl Acad. Sci. USA, 96, 1840–1845.pmid/10051556
23. Latta, L., Knapp, M., Sarmientos, P., Brefort, G., Becquart, J., Guerrier, L., Jung, G. and Mayaux, J. (1987) Synthesis and purification of mature human serum albumin from E. coli. Bio/Technol. 5, 1309–1314.
24. Mayfield, S.P., Yohn, C.B., Cohen, A. and Danon, A. (1995) Regulation of chloroplast gene expression. Annu. Rev. Plant Physiol. Plant Mol. Biol. 46, 147–166.
25. Mitraki, A. and King, J. (1989) Protein folding intermediates and inclusion body formation. Bio/Technol. 7, 690–697.
26. Ohtani, W., Ohda, T., Sumi, A., Kobayashi, K. and Ohmura, T. (1998) Analysis of Pichia pastoris components in recombinant human serum albumin by immunological assays and by HPLC with pulsed amperometric detection. Anal. Chem. 70, 425–429.pmid/9450369
27. Peters, T. (1995) The Albumin molecule. In: All About Albumin: Biochemistry, Genetics and Medical Applications. Chap. 6, pp. 251–284. San Diego, CA: Academic Press.
28. Petersen, C.E., Ha, C., Harohalli, K., Feix, J.B. and Bhagavan, N.V. (2000) A dynamic model for bilirrubin binding to human serum albumin. J. Biol. Chem. 275, 20985–20995.pmid/10764755
29. Petrides, D., Sapidou, E. and Calandranis, J. (1995) Computer aided process analysis and economic evaluation for biosynthetic human insulin production − a study case. Biotechnol. Bioeng. 48, 529–541.
30. Quirk, A.V., Geisow, M.J., Woodrow, J.R., Burton, S.J., Wood, P.C., Sutton, A.D., Johnson, R.A. and Dodsworth, N. (1989) Production of recombinant human serum albumin from Saccharomyces cerevisiae. Biotechnol. Appl. Biochem. 11, 273–287.pmid/2667569
31. Rozkov, A., Schweder, T., Veide, A. and Enfors, S.O. (2000) Dynamics of proteolysis and its influence on the accumulation of intracellular recombinant proteins. Enzyme Microbiol. Technol. 27, 743–748.
32. Ruf, S., Hermann, M., Berger, I.J., Carrer, H. and Bock, R. (2001) Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit. Nat. Biotechnol. 19, 870–875.pmid/11533648
33. Saunders, C.W., Schmidt, B.J., Mallonee, R.L. and Guyer, M.S. (1987) Secretion of human serum albumin from Bacillus subtilis. J. Bacteriol. 169, 2917–2925.pmid/3110129
34. Shively, J.M. and English, R.S. (1991) The carboxysome, a prokaryotic organelle: a mini review. Can. J. Bot. 69, 957–962.
35. Sijmons, P.C., Dekker, B.M., Schrammeijer, B., Verwoerd, T.C., Van Den Elzen, P.J. and Hoekema, A. (1990) Production of correctly processed human serum albumin in transgenic plants. Bio/Technol. 8, 217–221.
36. Staub, J.M., Garcia, B., Graves, J., Hajdukiewicz, P.T., Hunter, P., Nehra, N., Paradkar, V., Schlittler, M., Carroll, J.A., Spatola, L., Ward, D.Ye,, G. and Russell, D.A. (2000) High-yield production of a human therapeutic protein in tobacco chloroplasts. Nat. Biotechnol. 18, 333–338.pmid/10700152
37. Staub, J.M. and Maliga, P. (1993) Accumulation of D1 polypeptide in tobacco plastid is regulated via the untranslated region of the psbA mRNA. EMBO J. 12, 601–606.pmid/8440249
38. Staub, J.M. and Maliga, P. (1994) Translation of psbA mRNA is regulated by light via the 5′-untranslated region in tobacco plastids. Plant J. 6, 547–553.pmid/7987413
39. Tarelli, E., Mire-Sluis, A., Tivnann, H.A., Bolgiano, B., Crane, D.T., Gee, C., Lemercinier, X., Athayde, M.L., Sutcliffe, N., Corran, P.H. and Rafferty, B. (1998) Recombinant human albumin as a stabilizer for biological materials and for the preparation of international reference reagents. Biologicals, 26, 331–346.pmid/10403037
40. Vrekleij, A.J. and Leunissen, J.M., eds. (1989) Immuno-Gold Labeling in Cell Biology. Boca Raton, FL: CRC Press.
41. Watanabe, H., Kragh-Hansen, U., Tanase, S., Nakajou, K., Iwao, Y., Maruyama, T. and Otagiri, M. (2001a) Conformational stability and warfarin-binding properties of human serum albumin studied by recombinant mutants. Biochem. J. 357, 269–274.pmid/11415459
42. Watanabe, H., Yamasaki, K., Kragh-Hansen, U., Tanase, S., Harada, K., Suenaga, A. and Otagiri, M. (2001b) In vitro and in vivo properties of recombinant human serum albumin from Pichia pastoris purified by a method of short processing time. Pharm. Res. 18, 1775–1781.pmid/11785700
43. Zerges, W. (2000) Translation in chloroplasts. Biochimie, 82, 583–601.pmid/10946109