Single-molecule tracking of membrane molecules: Plasma membrane compartmentalization and dynamic assembly of raft-philic signaling molecules

Seminars in Immunology

Volumn 17, Issue 1, 2005, Pages 3-21

  Kusumi, Akihiro ^a   Ike, Hiroshi ^a   Nakada, Chieko ^a   Murase, Kotono ^a   Fujiwara, Takahiro ^a  

^a Institute for Advanced Research   (Japan)

Author keywords

Raft domains; Single fluorescent molecule video imaging; Single particle tracking; T cell receptor

Indexed keywords

ACTIN; LIPID; MEMBRANE PROTEIN; PROTEIN KINASE LCK; T LYMPHOCYTE RECEPTOR;

ANALYTIC METHOD; BINDING SITE; CELL COMPARTMENTALIZATION; CELL MEMBRANE; CELL STRUCTURE; CROSS LINKING; DIFFUSION COEFFICIENT; FLUORESCENCE; FLUORESCENCE MICROSCOPY; IMAGING; IMMUNOLOGY; MOLECULAR DYNAMICS; MONITORING; NONHUMAN; OLIGOMERIZATION; PROTEIN DOMAIN; RATING SCALE; REVIEW; SIGNAL TRANSDUCTION;

EID: 9644284452     PISSN: 10445323     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.smim.2004.09.004     Document Type: Article

References (168)

1. M.P. Sheetz, S. Turney, H. Qian, and E.L. Elson Nanometre-level analysis demonstrates that lipid flow does not drive membrane glycoprotein movements Nature 340 1989 284 288
2. M. De Brabander, R. Nuydens, A. Ishihara, B. Holifield, K. Jacobson, and H. Geerts Lateral diffusion and retrograde movements of individual cell surface components on single motile cells observed with Nanovid microscopy J Cell Biol 112 1991 111 124
3. M. Edidin, S.C. Kuo, and M.P. Sheetz Lateral movements of membrane glycoproteins restricted by dynamic cytoplasmic barriers Science 254 1991 1379 1382
4. D.F. Kucik, S.C. Kuo, E.L. Elson, and M.P. Sheetz Preferential attachment of membrane glycoproteins to the cytoskeleton at the leading edge of lamella J Cell Biol 114 1991 1029 1036
5. S.C. Kuo, and M.P. Sheetz Optical tweezers in cell biology Trends Cell Biol 2 1992 116 118
6. A. Kusumi, Y. Sako, and M. Yamamoto Confined lateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). Effects of calcium-induced differentiation in cultured epithelial cells Biophys. J. 65 1993 2021 2040
7. M.J. Saxton, and K. Jacobson Single-particle tracking: applications to membrane dynamics Annu Rev Biophys Biomol Struct 26 1997 379 399
8. M.J. Saxton Single-particle tracking: the distribution of diffusion coefficients Biophys J 72 1997 1744 1753
9. C.E. Schmidt, A.F. Horwitz, D.A. Lauffenburger, and M.P. Sheetz Integrin-cytoskeletal interactions in migrating fibroblasts are dynamic, asymmetric, and regulated J Cell Biol 123 1993 977 991
10. Y. Sako, and A. Kusumi Barriers for lateral diffusion of transferrin receptor in the plasma membrane as characterized by receptor dragging by laser tweezers: fence versus tether J Cell Biol 129 1995 1559 1574
11. T. Schmidt, G.J. Schütz, W. Baumgartner, H.J. Grüber, and H. Schindler Characterization of photophysics and mobility of single molecules in a fluid lipid-membrane J Phys Chem 99 1995 17662 17668
12. A. Kusumi, Y. Sako, T. Fujiwara, and M. Tomishige Application of laser tweezers to studies of the fences and tethers of the membrane skeleton that regulate the movements of plasma membrane proteins Methods Cell Biol 55 1998 173 194
13. Y. Sako, S. Minoghchi, and T. Yanagida Single-molecule imaging of EGFR signalling on the surface of living cells Nat Cell Biol 2 2000 168 172
14. G.J. Schütz, G. Kada, V.P. Pastushenko, and H. Schindler Properties of lipid microdomains in a muscle cell membrane visualized by single molecule microscopy EMBO J 19 2000 892 901
15. R. Iino, I. Koyama, and A. Kusumi Single molecule imaging of green fluorescent proteins in living cells: E-cadherin forms oligomers on the free cell surface Biophys J 80 2001 2667 2677
16. G.S. Harms, L. Cognet, and P.H. Lommerse Single-molecule imaging of L-type Ca(2+) channels in live cells Biophys J 81 2001 2639 2646
17. H. Murakoshi, R. Iino, and T. Kobayashi Single-molecule imaging analysis of Ras activation in living cells Proc Natl Acad Sci USA 101 2004 7317 7322
18. M. Pasenkiewicz-Gierula, W.K. Subczynski, and A. Kusumi Influence of phospholipid unsaturation on the cholesterol distribution in membranes Biochimie 73 1991 1311 1316
19. T. Fujiwara, K. Ritchie, H. Murakoshi, K. Jacobson, and A. Kusumi Phospholipids undergo hop diffusion in compartmentalized cell membrane J Cell Biol 157 2002 1071 1081
20. A. Kusumi, and Y. Sako Cell surface organization by the membrane skeleton Curr Opin Cell Biol 8 1996 566 574
21. J. Gelles, B.J. Schnapp, and M.P. Sheetz Tracking kinesin-driven movements with nanometre-scale precision Nature 331 1988 450 453
22. M. Tomishige, Y. Sako, and A. Kusumi Regulation mechanism of the lateral diffusion of band 3 in erythrocyte membranes by the membrane skeleton J Cell Biol 142 1998 989 1000
23. C. Nakada, K. Ritchie, and Y. Oba Accumulation of anchored proteins forms membrane diffusion barriers during neuronal polarization Nat Cell Biol 5 2003 626 632
24. S.J. Singer, and G.L. Nicolson The fluid mosaic model of the structure of cell membranes Science 175 1972 720 731
25. P.G. Saffman, and M. Delbrück Brownian motion in biological membranes Proc Natl Acad Sci USA 72 1975 3111 3113
26. R. Peters, and R.J. Cherry Lateral and rotational diffusion of bacteriorhodopsin in lipid bilayers: experimental test of the Saffman-Delbrück equations Proc Natl Acad Sci USA 79 1982 4317 4321
27. D. Axelrod, P. Ravdin, and D.E. Koppel Lateral motion of fluorescently labeled acetylcholine receptors in membranes of developing muscle fibers Proc Natl Acad Sci USA 73 1976 4594 4598
28. M.P. Sheetz, M. Schindler, and D.E. Koppel Lateral mobility of integral membrane proteins is increased in spherocytic erythrocytes Nature 285 1980 510 511
29. M.P. Sheetz Membrane skeletal dynamics - role in modulation of red-cell deformability, mobility of transmembrane proteins, and shape Semin Hematol 20 1983 175 188
30. C.H. Chang, H. Takeuchi, T. Ito, K. Machida, and S. Ohnishi Lateral mobility of erythrocyte membrane proteins studied by the fluorescence photobleaching recovery technique J Biochem 90 1981 997 1004
31. D.W. Tank, E.S. Wu, P.R. Meers, and W.W. Webb Lateral diffusion of gramicidin C in phospholipid multibilayers. Effects of cholesterol and high gramicidin concentration Biophys J 40 1982 129 135
32. E.S. Wu, D.W. Tank, and W.W. Webb Unconstrained lateral diffusion of concanavalin A receptors on bulbous lymphocytes Proc Natl Acad Sci USA 79 1982 4962 4966
33. E. Yechiel, and M. Edidin Micrometer-scale domains in fibroblast plasma membranes J Cell Biol 105 1987 755 760
34. A. Tsuji, K. Kawasaki, S. Ohnishi, H. Merkle, and A. Kusumi Regulation of band 3 mobilities in erythrocyte ghost membranes by protein association and cytoskeletal meshwork Biochemistry 27 1988 7447 7452
35. A. Tsuji, and S. Ohnishi Restriction of the lateral motion of band 3 in the erythrocyte membrane by the cytoskeletal network: dependence on spectrin association state Biochemistry 25 1986 6133 6139
36. M.J. Saxton Lateral diffusion in an archipelago. Effects of impermeable patches on diffusion in a cell membrane Biophys J 39 1982 165 173
37. M.J. Saxton Lateral diffusion in an archipelago. The effect of mobile obstacles Biophys J 52 1987 989 997
38. M.J. Saxton The spectrin network as a barrier to lateral diffusion in erythrocytes. A percolation analysis Biophys J 55 1989 21 28
39. M.J. Saxton Lateral diffusion in an archipelago. Distance dependence of the diffusion coefficient Biophys J 56 1989 615 622
40. M.J. Saxton The membrane skeleton of erythrocytes. A percolation model Biophys J 57 1990 1167 1177
41. M.J. Saxton Single-particle tracking: effects of corrals Biophys J 69 1995 389 398
42. M.J. Saxton Anomalous diffusion due to binding: a Monte Carlo study Biophys J 70 1996 1250 1262
43. M.J. Saxton Anomalous subdiffusion in fluorescence photobleaching recovery: a Monte Carlo study Biophys J 81 2001 2226 2240
44. J.F. Nagle Long tail kinetics in biophysics? Biophys J 63 1992 366 370
45. T.J. Feder, I. Brust-Mascher, J.P. Slattery, B. Baird, and W.W. Webb Constrained diffusion or immobile fraction on cell surfaces: a new interpretation Biophys J 70 1996 2767 2773
46. K. Murase, T. Fujiwara, and Y. Umemura Ultrafine membrane compartments for molecular diffusion as revealed by single molecule techniques Biophys J 86 2004 4075 4093
47. O. Hegener, L. Prenner, F. Runkel, S.L. Baader, J. Kappler, and H. Haberlein Dynamics of beta2-adrenergic receptor-ligand complexes on living cells Biochemistry 43 2004 6190 6199
48. J.L. Thomas, D. Holowka, B. Baird, and W.W. Webb Large-scale co-aggregation of fluorescent lipid probes with cell surface proteins J Cell Biol 125 1994 795 802
49. M. De Brabander, G. Geuens, R. Nuydens, M. Moeremans, and J. De Mey Probing microtubule-dependent intracellular motility with nanometre particle video ultramicroscopy (nanovid ultramicroscopy) Cytobios 43 1985 273 283
50. M. De Brabander, R. Nuydens, H. Geerts, and C.R. Hopkins Dynamic behavior of the transferrin receptor followed in living epidermoid carcinoma (A431) cells with nanovid microscopy Cell Motil Cytoskelet 9 1988 30 47
51. B.J. Schnapp, J. Gelles, and M.P. Sheetz Nanometer-scale measurements using video light microscopy Cell Motil Cytoskelet 10 1988 47 53
52. D.F. Kucik, E.L. Elson, and M.P. Sheetz Forward transport of glycoproteins on leading lamellipodia in locomoting cells Nature 340 1989 315 317
53. Y. Sako, and A. Kusumi Compartmentalized structure of the plasma membrane for receptor movements as revealed by a nanometer-level motion analysis J Cell Biol 125 1994 1251 1264
54. Y. Sako, A. Nagafuchi, S. Tsukita, M. Takeichi, and A. Kusumi Cytoplasmic regulation of the movement of E-cadherin on the free cell surface as studied by optical tweezers and single particle tracking: corralling and tethering by the membrane skeleton J Cell Biol 140 1998 1227 1240
55. D.W. Tank, E.S. Wu, and W.W. Webb Enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints J Cell Biol 92 1982 207 212
56. M.S. Paller Lateral mobility of Na,K-ATPase and membrane lipids in renal cells. Importance of cytoskeletal integrity J Membr Biol 142 1994 127 135
57. M. Takeuchi, H. Miyamoto, Y. Sako, H. Komizu, and A. Kusumi Structure of the erythrocyte membrane skeleton as observed by atomic force microscopy Biophys J 74 1998 2171 2183
58. M. Vrljic, S.Y. Nishimura, S. Brasselet, W.E. Moerner, and H.M. McConnell Translational diffusion of individual class II MHC membrane proteins in cells Biophys J 83 2002 2681 2692
59. K. Schmidt, and B.J. Nichols A barrier to lateral diffusion in the cleavage furrow of dividing mammalian cells Curr Biol 14 2004 1002 1006
60. S.J. Bussell, D.A. Hammer, and D.L. Koch The effect of hydrodynamic interactions on the tracer and gradient diffusion of integral membrane-proteins in lipid bilayers J Fluid Mech 258 1994 167 190
61. S.J. Bussell, D.L. Koch, and D.A. Hammer Effect of hydrodynamic interactions on the diffusion of integral membrane proteins: diffusion in plasma membranes Biophys J 68 1995 1836 1849
62. R.N. Ghosh, and W.W. Webb Automated detection and tracking of individual and clustered cell surface low density lipoprotein receptor molecules Biophys J 66 1994 1301 1318
63. M.J. Saxton Single-particle tracking: models of directed transport Biophys J 67 1994 2110 2119
64. G.B. Warren, M.D. Houslay, J.C. Metcalfe, and N.J. Birdsall Cholesterol is excluded from the phospholipid annulus surrounding an active calcium transport protein Nature 255 1975 684 687
65. A. Kusumi, I. Koyama-Honda, and K. Suzuki Molecular dynamics and interactions for creation of stimulation-induced stabilized rafts from small unstable steady-state rafts Traffic 5 2004 213 230
66. A.K. Kenworthy, B.J. Nichols, and C.L. Remmert Dynamics of putative raft-associated proteins at the cell surface J Cell Biol 165 2004 735 746
67. J.E. Schnitzer, D.P. McIntosh, A.M. Dvorak, J. Liu, and P. Oh Separation of caveolae from associated microdomains of GPI-anchored proteins Science 269 1995 1435 1439
68. R.G. Anderson The caveolae membrane system Annu Rev Biochem 67 1998 199 225
69. R.G. Parton Cell biology. Life without caveolae Science 293 2001 2404 2405
70. K.G. Rothberg, J.E. Heuser, W.C. Donzell, Y.S. Ying, J.R. Glenney, and R.G. Anderson Caveolin a protein component of caveolae membrane coats Cell 68 1992 673 682
71. R.G. Parton Ultrastructural localization of gangliosides; GM1 is concentrated in caveolae J Histochem Cytochem 42 1994 155 166
72. M. Murata, J. Peranen, R. Schreiner, F. Wieland, T.V. Kurzchalia, and K. Simons VIP21/caveolin is a cholesterol-binding protein Proc Natl Acad Sci USA 92 1995 10339 10343
73. S. Mayor, K.G. Rothberg, and F.R. Maxfield Sequestration of GPI-anchored proteins in caveolae triggered by cross-linking Science 264 1994 1948 1951
74. T. Harder, P. Scheiffele, P. Verkade, and K. Simons Lipid domain structure of the plasma membrane revealed by patching of membrane components J Cell Biol 141 1998 929 942
75. T. Harder, and K. Simons Clusters of glycolipid and glycosylphosphatidylinositol-anchored proteins in lymphoid cells: accumulation of actin regulated by local tyrosine phosphorylation Eur J Immunol 29 1999 556 562
76. E.D. Sheets, G.M. Lee, R. Simson, and K. Jacobson Transient confinement of a glycosylphosphatidylinositol-anchored protein in the plasma membrane Biochemistry 36 1997 12449 12458
77. A. Pralle, P. Keller, E.L. Florin, K. Simons, and J.K. Horber Sphingolipid-cholesterol rafts diffuse as small entities in the plasma membrane of mammalian cells J Cell Biol 148 2002 997 1008
78. C. Dietrich, B. Yang, T. Fujiwara, A. Kusumi, and K. Jacobson Relationship of lipid rafts to transient confinement zones detected by single particle tracking Biophys J 82 2002 274 284
79. K. Suzuki, and M.P. Sheetz Binding of cross-linked glycosylphosphatidylinositol-anchored proteins to discrete actin-associated sites and cholesterol-dependent domains Biophys J 81 2001 2181 2189
80. D. Holowka, E.D. Sheets, and B. Baird Interactions between Fc(epsilon)RI and lipid raft components are regulated by the actin cytoskeleton J Cell Sci 113 2000 1009 10019
81. J. Matko, A. Bodnar, and G. Vereb GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines Eur J Biochem 269 2002 1199 1208
82. A. Samsonov, I. Mikhalyov, and F. Cohen Detection of rafts in biological membranes Biophys J 82 2002 521a.
83. D.E. Shvartsman, M. Kotler, R.D. Tall, M.G. Roth, and Y.I. Henis Differently anchored influenza hemagglutinin mutants display distinct interaction dynamics with mutual rafts J Cell Biol 163 2003 879 888
84. L.M. Pierini, R.J. Eddy, M. Fuortes, S. Seveau, C. Casulo, and F.R. Maxfield Membrane lipid organization is critical for human neutrophil polarization J Biol Chem 278 2003 10831 10841
85. L.M. Pierini, and F.R. Maxfield Flotillas of lipid rafts fore and aft Proc Natl Acad Sci USA 98 2001 9471 9473
86. F.R. Maxfield Plasma membrane microdomains Curr Opin Cell Biol 14 2002 483 487
87. I.A. Prior, C. Muncke, R.G. Parton, and J.F. Hancock Direct visualization of Ras proteins in spatially distinct cell surface microdomains J Cell Biol 160 2003 165 170
88. R.G. Parton, and J.F. Hancock Lipid rafts and plasma membrane microorganization: insights from Ras Trends Cell Biol 14 2004 141 147
89. A.K. Kenworthy, N. Petranova, and M. Edidin High-resolution FRET microscopy of cholera toxin B-subunit and GPI-anchored proteins in cell plasma membranes Mol Biol Cell 11 2000 1645 1655
90. K. Kawasaki, J.J. Yin, W.K. Subczynski, J.S. Hyde, and A. Kusumi Pulse EPR detection of lipid exchange between protein-rich raft and bulk domains in the membrane: methodology development and its application to studies of influenza viral membrane Biophys J 80 2001 738 748
91. R. Varma, and S. Mayor GPI-anchored proteins are organized in submicron domains at the cell surface Nature 394 1998 798 801
92. P. Sharma, R. Varma, R.C. Sarasij, I.G. Krishnamoorthy, M. Rao, and S. Mayor Nanoscale organization of multiple GPI-anchored proteins in living cell membranes Cell 116 2004 577 589
93. B.J. Nichols GM1-containing lipid rafts are depleted within clathrin-coated pits Curr Biol 13 2003 686 690
94. A.K. Kenworthy, and M. Edidin Distribution of a glycosylphosphatidylinositol-anchored protein at the apical surface of MDCK cells examined at a resolution of <100 Å using imaging fluorescence resonance energy transfer [erratum appears in J Cell Biol 1998;142(3):following 881] J Cell Biol 142 1998 69 84
95. A. Radhakrishnan, T.G. Anderson, and H.M. McConnell Condensed complexes, rafts, and the chemical activity of cholesterol in membranes Proc Natl Acad Sci USA 97 2000 12422 12427
96. A. Radhakrishnan, X.M. Li, R.E. Brown, and H.M. McConnell Stoichiometry of cholesterol-sphingomyelin condensed complexes in monolayers Biochim Biophys Acta 1511 2001 1 6
97. T.G. Anderson, and H.M. McConnell Condensed complexes and the calorimetry of cholesterol-phospholipid bilayers Biophys J 81 2001 2774 2785
98. T.G. Anderson, and H.M. McConnell A thermodynamic model for extended complexes of cholesterol and phospholipid Biophys J 83 2003 2039 2052
99. T.M. Okonogi, A. Radhakrishnan, and H.M. McConnell Two fatty acids can replace one phospholipid in condensed complexes with cholesterol Biochim Biophys Acta 1564 2002 1 4
100. H.M. McConnell, and A. Radhakrishnan Condensed complexes of cholesterol and phospholipids Biochim Biophys Acta 1610 2003 159 173
101. H.M. McConnell, and M. Vrljic Liquid-liquid immiscibility in membranes Annu Rev Biophys Biomol Struct 32 2003 469 492
102. K. Suzuki, F. Sanematsu, T. Fujiwara, M. Edidin, and A. Kusumi Rapid, continual formation/dispersion of raft-like domains in the resting cell membrane Mol Biol Cell 12 2001 470a
103. K. Suzuki, F. Sanematsu, T. Fujiwara, M. Edidin, and A. Kusumi Stimulation-induced formation of temporal but stabilized rafts Biophys J 82 2002 348a
104. K. Suzuki, F. Sanematsu, T. Fujiwara, K. Ritchie, M. Edidin, and A. Kusumi Crosslinking of a GPI-anchored protein creates signaling rafts from smaller, transient, lipid rafts Biophys J 84 2003 487a
105. R.G. Anderson, and K. Jacobson A role for lipid shells in targeting proteins to caveolae, rafts, and other lipid domains Science 296 2002 1821 1825
106. S.K. Pierce Lipid rafts and B-cell activation Nat Rev Immunol 2 2002 96 105
107. C. Dietrich, Z.N. Volovyk, M. Levi, N.L. Thompson, and K. Jacobson Partitioning of Thy-1, GM1, and cross-linked phospholipid analogs into lipid rafts reconstituted in supported model membrane monolayers Proc Natl Acad Sci USA 98 2001 10642 10647
108. R.M. Young, D. Holowka, and B. Baird A lipid raft environment enhances Lyn kinase activity by protecting the active site tyrosine from dephosphorylation J Biol Chem 278 2003 20746 20752
109. E.D. Sheets, D. Holowka, and B. Baird Critical role for cholesterol in Lyn-mediated tyrosine phosphorylation of FcepsilonRI and their association with detergent-resistant membranes J Cell Biol 145 1999 877 887
110. K.A. Field, D. Holowka, and B. Baird Fc epsilon RI-mediated recruitment of p53/56lyn to detergent-resistant membrane domains accompanies cellular signaling Proc Natl Acad Sci USA 92 1995 9201 9205
111. B. Baird, E.D. Sheets, and D. Holowka How does the plasma membrane participate in cellular signaling by receptors for immunoglobulin E? Biophys Chem 82 1999 109 1019
112. A. Cherukuri, R.H. Carter, and S. Brooks B cell signaling is regulated by induced palmitoylation of CD81 J Biol Chem 279 2004 31973 31982
113. A. Cherukuri, T. Shoham, and H.W. Sohn The tetraspanin CD81 is necessary for partitioning of coligated CD19/CD21-B cell antigen receptor complexes into signaling-active lipid rafts J Immunol 172 2004 370 380
114. M. Dykstra, A. Cherukuri, H.W. Sohn, S.J. Tzeng, and S.K. Pierce Location is everything: lipid rafts and immune cell signaling Annu Rev Immunol 21 2003 457 481
115. A. Stoddart, M.L. Dykstra, B.K. Brown, W. Song, S.K. Pierce, and F.M. Brodsky Lipid rafts unite signaling cascades with clathrin to regulate BCR internalization Immunity 17 2002 451 462
116. P.W. Janes, S.C. Ley, and A.I. Magee Aggregation of lipid rafts accompanies signaling via the T cell antigen receptor J Cell Biol 147 1999 447 461
117. K. Simons, and E. Ikonen Functional rafts in cell membranes Nature 387 1997 569 572
118. W.K. Subczynski, W.E. Antholine, J.S. Hyde, and A. Kusumi Microimmiscibility and three-dimensional dynamic structures of phosphatidylcholine-cholesterol membranes: translational diffusion of a copper complex in the membrane Biochemistry 29 1990 7936 7945
119. T. Gil, J.H. Ipsen, O.G. Mouritsen, M.C. Sabra, M.M. Sperotto, and M.J. Zuckermann Theoretical analysis of protein organization in lipid membranes Biochim Biophys Acta 1376 1998 245 266
120. M.S. Bretscher, and S. Munro Cholesterol and the Golgi apparatus Science 261 1993 1280 1281
121. A. Kusumi, and J.S. Hyde Spin-label saturation-transfer electron spin resonance detection of transient association of rhodopsin in reconstituted membranes Biochemistry 21 1982 5978 5983
122. J.M. East, D. Melville, and A.G. Lee Exchange rates and numbers of annular lipids for the calcium and magnesium ion dependent adenosinetriphosphatase Biochemistry 24 1985 2615 2623
123. L.I. Horvath, P.J. Brophy, and D. Marsh Exchange rates at the lipid-protein interface of myelin proteolipid protein studied by spin-label electron spin resonance Biochemistry 27 1988 46 52
124. S.J. Bussell, D.L. Koch, and D.A. Hammer Effect of hydrodynamic interactions on the diffusion of integral membrane proteins: tracer diffusion in organelle and reconstituted membranes Biophys J 68 1995 1828 1835
125. T. Dodd, D. Hammer, A. Sangani, and D. Koch Numerical simulations of the effect of hydrodynamic interactions on diffusivities of integral membrane proteins J Fluid Mech 293 1995 147 180
126. I. Ashikawa, J.J. Yin, W.K. Subczynski, T. Kouyama, J.S. Hyde, and A. Kusumi Molecular organization and dynamics in bacteriorhodopsin-rich reconstituted membranes: discrimination of lipid environments by the oxygen transport parameter using a pulse ESR spin-labeling technique Biochemistry 33 1994 4947 4952
127. A. Kusumi, T. Sakaki, T. Yoshizawa, and S. Ohnishi Protein-lipid interaction in rhodopsin recombinant membranes as studied by protein rotational mobility and lipid alkyl chain flexibility measurements J Biochem (Tokyo) 88 1980 1103 1111
128. P.F. Knowles, A. Watts, and D. Marsh Spin-label studies of lipid immobilization in dimyristoylphosphatidylcholine-substituted cytochrome oxidase Biochemistry 18 1979 4480 4487
129. P.F. Knowles, A. Watts, and D. Marsh Spin-label studies of head-group specificity in the interaction of phospholipids with yeast cytochrome oxidase Biochemistry 20 1981 5888 5894
130. M.M. Sperotto, and O.G. Mouritsen Monte Carlo simulation studies of lipid order parameter profiles near integral membrane proteins Biophys J 59 1991 261 270
131. P.F. Almeida, W.L. Vaz, and T.E. Thompson Lateral diffusion in the liquid phases of dimyristoylphosphatidylcholine/cholesterol lipid bilayers: a free volume analysis Biochemistry 31 1992 6739 6747
132. L.P. Kane, J. Lin, and A. Weiss Signal transduction by the TCR for antigen Curr Opin Immunol 12 2000 242 249
133. W. Zhang, and L.E. Samelson The role of membrane-associated adaptors in T cell receptor signalling Semin Immunol 12 2000 35 41
134. G. Iezzi, K. Karjalainen, and A. Lanzavecchia The duration of antigenic stimulation determines the fate of naive and effector T cells Immunity 8 1998 89 95
135. J.B. Huppa, M. Gleimer, C. Sumen, and M.M. Davis Continuous T cell receptor signaling required for synapse maintenance and full effector potential Nat Immunol 4 2003 749 755
136. G.J. Kersh, E.N. Kersh, D.H. Fremont, and P.M. Allen High- and low-potency ligands with similar affinities for the TCR: the importance of kinetics in TCR signaling Immunity 9 1998 817 826
137. C. Pioli, L. Gatta, D. Frasca, and G. Doria Cytotoxic T lymphocyte antigen 4 (CTLA-4) inhibits CD28-induced IkappaBalpha degradation and RelA activation Eur J Immunol 29 1999 856 863
138. B.A. Freiberg, H. Kupfer, and W. Maslanik Staging and resetting T cell activation in SMACs Nat Immunol 3 2002 911 917
139. M. Krogsgaard, J.B. Huppa, M.A. Purbhoo, and M.M. Davis Linking molecular and cellular events in T-cell activation and synapse formation Semin Immunol 15 2003 307 315
140. M. Krogsgaard, N. Prado, and E.J. Adams Evidence that structural rearrangements and/or flexibility during TCR binding can contribute to T cell activation Mol Cell 12 2003 1367 1378
141. A. Viola, S. Schroeder, Y. Sakakibara, and A. Lanzavecchia T lymphocyte costimulation mediated by reorganization of membrane microdomains Science 283 1999 680 682
142. L.C. Hartgroves, J. Lin, H. Langen, T. Zech, A. Weiss, and T. Harder Synergistic assembly of linker for activation of T cells signaling protein complexes in T cell plasma membrane domains J Biol Chem 278 2003 20389 20394
143. S.B. Taner, B. Onfelt, N.J. Pirinen, F.E. McCann, A.I. Magee, and D.M. Davis Control of immune responses by trafficking cell surface proteins, vesicles and lipid rafts to and from the immunological synapse Traffic 5 2004 651 661
144. H. Ike, A. Kosugi, and A. Kato Mechanism of Lck recruitment to the T-cell receptor cluster as studied by single-molecule-fluorescence video imaging ChemPhysChem. 4 2003 620 626
145. C.R. Monks, B.A. Freiberg, H. Kupfer, N. Sciaky, and A. Kupfer Three-dimensional segregation of supramolecular activation clusters in T cells Nature 395 1998 82 86
146. A.D. Holdorf, K.H. Lee, W.R. Burack, P.M. Allen, and A.S. Shaw Regulation of Lck activity by CD4 and CD28 in the immunological synapse Nat Immunol 3 2002 259 264
147. P.S. Kabouridis, J. Janzen, A.L. Magee, and S.C. Ley Cholesterol depletion disrupts lipid rafts and modulates the activity of multiple signaling pathways in T lymphocytes Eur J Immunol 30 2000 954 963
148. Q.J. Li, A.R. Dinner, and S. Qi CD4 enhances T cell sensitivity to antigen by coordinating Lck accumulation at the immunological synapse Nat Immunol 5 2004 791 799
149. M. Moran, and M.C. Miceli Engagement of GPI-linked CD48 contributes to TCR signals and cytoskeletal reorganization: a role for lipid rafts in T cell activation Immunity 9 1998 787 796
150. S.C. Bunnell, V. Kapoor, R.P. Trible, W. Zhang, and L.E. Samelson Dynamic actin polymerization drives T cell receptor-induced spreading: a role for the signal transduction adaptor LAT Immunity 14 2001 315 329
151. S.C. Bunnell, D.I. Hong, and J.R. Kardon T cell receptor ligation induces the formation of dynamically regulated signaling assemblies J Cell Biol 158 2002 1263 1275
152. P.W. Kim, Z.Y. Sun, S.C. Blacklow, G. Wagner, and M.J. Eck A zinc clasp structure tethers Lck to T cell coreceptors CD4 and CD8 Science 301 2003 1725 1728
153. M.J. Miller, S.H. Wei, I. Parker, and M.D. Cahalan Two-photon imaging of lymphocyte motility and antigen response in intact lymph node Science 296 2002 1869 1873
154. M.D. Cahalan, I. Parker, S.H. Wei, and M.J. Miller Real-time imaging of lymphocytes in vivo Curr Opin Immunol 15 2003 372 377
155. M.L. Dustin Adhesive bond dynamics in contacts between T lymphocytes and glass-supported planar bilayers reconstituted with the immunoglobulin-related adhesion molecule CD58 J Biol Chem 272 1997 15782 15788
156. J.T. Groves, and M.L. Dustin Supported planar bilayers in studies on immune cell adhesion and communication J Immunol Methods 278 2003 19 32
157. T. Kobayashi, B. Storrie, K. Simons, and C.G. Dotti A functional barrier to movement of lipids in polarized neurons Nature 359 1992 647 650
158. A.H. Futerman, R. Khanin, and L.A. Segel Lipid diffusion in neurons Nature 362 1993 119
159. B. Winckler, and M.M. Poo No diffusion barrier at axon hillock Nature 379 1996 213
160. B. Winckler, and I. Mellman Neuronal polarity: controlling the sorting and diffusion of membrane components Neuron 23 1999 637 640
161. B. Winckler, P. Forscher, and I. Mellman A diffusion barrier maintains distribution of membrane proteins in polarized neurons Nature 397 1999 698 701
162. E. Kordeli, S. Lambert, and V. Bennett Ankyrin G. A new ankyrin gene with neural-specific isoforms localized at the axonal initial segment and node of Ranvier J Biol Chem 270 1995 2352 2359
163. J.Q. Davis, S. Lambert, and V. Bennett Molecular composition of the node of Ranvier: identification of ankyrin-binding cell adhesion molecules neurofascin (mucin+/third FNIII domain-) and NrCAM at nodal axon segments J Cell Biol 135 1996 1355 1367
164. D. Zhou, S. Lambert, P.L. Malen, S. Carpenter, L.M. Boland, and V. Bennett Ankyrin G is required for clustering of voltage-gated Na channels at axon initial segments and for normal action potential firing J Cell Biol 143 1998 1295 1304
165. J.J. Garrido, P. Giraud, and E. Carlier A targeting motif involved in sodium channel clustering at the axonal initial segment Science 300 2004 2091 2094
166. M.P. Fache, A. Moussif, F. Fernandes, P. Giraud, J.J. Garrido, and B. Dargent Endocytotic elimination and domain-selective tethering constitute a potential mechanism of protein segregation at the axonal initial segment J Cell Biol 166 2004 571 578
167. E. Matsumoto, and J. Rosenbluth Plasma membrane structure at the axon hillock, initial segment and cell body of frog dorsal root ganglion cells J Neurocytol 14 1985 731 747
168. S. Berghs, D. Aggujaro, and R. Dirkx Jr. BetaIV spectrin, a new spectrin localized at axon initial segments and nodes of Ranvier in the central and peripheral nervous system J Cell Biol 151 2000 985 1002