Enabling Graded and Large-Scale Multiplex of Desired Genes Using a Dual-Mode dCas9 Activator in Saccharomyces cerevisiae

ACS Synthetic Biology

Volumn 6, Issue 10, 2017, Pages 1931-1943

  Deaner, Matthew ^a   Mejia, Julio ^a   Alper, Hal S ^a,b  

^a The University of Texas at Austin   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

CRISPR dCas9; gene multiplexing; inducible sgRNAs; metabolic engineering; ribozymes

Indexed keywords

CAS9 PROTEIN; ENDONUCLEASE; GALACTOSE; GUIDE RNA; RIBOZYME; RNA POLYMERASE II; UNCLASSIFIED DRUG;

ARTICLE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; GENE CONTROL; GENE EXPRESSION; GENE LOCUS; GENE REPRESSION; HOMOLOGOUS RECOMBINATION; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROMOTER REGION; SACCHAROMYCES CEREVISIAE; START CODON; TRANSCRIPTION ELONGATION; CRISPR CAS SYSTEM; GENETICS; METABOLIC ENGINEERING; PROCEDURES; TRANSCRIPTION INITIATION;

CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; METABOLIC ENGINEERING; PROMOTER REGIONS, GENETIC; RNA, CATALYTIC; SACCHAROMYCES CEREVISIAE; TRANSCRIPTIONAL ACTIVATION;

EID: 85031934030     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.7b00163     Document Type: Article

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