Homology-Integrated CRISPR-Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae

ACS Synthetic Biology

Volumn 4, Issue 5, 2015, Pages 585-594

  Bao, Zehua ^a   Xiao, Han ^a   Liang, Jing ^a   Zhang, Lu ^a   Xiong, Xiong ^a   Sun, Ning ^a,b   Si, Tong ^a   Zhao, Huimin ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b HARVARD UNIVERSITY   (United States)

Author keywords

CRISPR Cas; gene knockout; genome editing; multiple gene disruption; Saccharomyces cerevisiae

Indexed keywords

ACTIVATING TRANSCRIPTION FACTOR 2; AMINO ACID TRANSPORTER; CAN1 PROTEIN, S CEREVISIAE; LYP1 PROTEIN, S CEREVISIAE; RNA; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; CRISPR CAS SYSTEM; GENE DISRUPTION; GENE SEQUENCE; GENOME; HOMOLOGOUS RECOMBINATION; KNOCKOUT GENE; MULTIGENE FAMILY; NONHUMAN; PLASMID; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; BIOSYNTHESIS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; GENETICS; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE;

SACCHAROMYCES CEREVISIAE;

AMINO ACID TRANSPORT SYSTEMS, BASIC; BASE SEQUENCE; BIOSYNTHETIC PATHWAYS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; HOMOLOGOUS RECOMBINATION; MOLECULAR SEQUENCE DATA; RNA; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 84929572600     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/sb500255k     Document Type: Article

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