CRISPR interference as a titratable, trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002

Metabolic Engineering

Volumn 38, Issue , 2016, Pages 170-179

  Gordon, Gina C ^a,b   Korosh, Travis C ^a,b   Cameron, Jeffrey C ^c   Markley, Andrew L ^a   Begemann, Matthew B ^a,b   Pfleger, Brian F ^a,b  

^a University of Wisconsin Madison   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

^c UNIVERSITY OF COLORADO   (United States)

Author keywords

Carboxysome; Chemical production; CRISPRi; Cyanobacteria; Lactate; Phycobilisome; Synthetic biology; Tunable

Indexed keywords

CARBON DIOXIDE; CELL CULTURE; GENE EXPRESSION; GENES; METABOLISM; NUCLEIC ACIDS; PHYSIOLOGY; PROTEINS; TRANSCRIPTION;

CARBOXYSOMES; CHEMICAL PRODUCTION; CRISPRI; CYANOBACTERIA; LACTATE; PHYCOBILISOME; SYNTHETIC BIOLOGY; TUNABLE;

METABOLIC ENGINEERING;

ALLOPHYCOCYANIN; CRISPR ASSOCIATED PROTEIN; GUIDE RNA; LACTATE DEHYDROGENASE; LACTATE SODIUM; PHYCOCYANIN; TRANSACTIVATOR PROTEIN;

ARTICLE; AUXOTROPHY; BASE PAIRING; CARBON METABOLISM; CELL DENSITY; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; ESCHERICHIA COLI; ESSENTIAL GENE; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GENE REPRESSION; LIGHT HARVESTING SYSTEM; METABOLIC ENGINEERING; NITROGEN FIXATION; NONHUMAN; PHOTOSYNTHESIS; PHOTOSYSTEM I; PHYCOBILISOME; PRIORITY JOURNAL; PROMOTER REGION; STREPTOCOCCUS PYOGENES; SYNECHOCOCCUS; SYNTHETIC BIOLOGY; BIOLOGICAL MODEL; BIOSYNTHESIS; COMPUTER SIMULATION; ETHICS; GENE EDITING; GENE EXPRESSION REGULATION; GENE SILENCING; GENETIC ENHANCEMENT; GENETICS; METABOLISM; PHYSIOLOGY; PROCEDURES; SIGNAL TRANSDUCTION; SPECIES DIFFERENCE;

BIOSYNTHETIC PATHWAYS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; COMPUTER SIMULATION; GENE EDITING; GENE EXPRESSION REGULATION, BACTERIAL; GENE SILENCING; GENETIC ENHANCEMENT; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; MODELS, BIOLOGICAL; SIGNAL TRANSDUCTION; SPECIES SPECIFICITY; STREPTOCOCCUS PYOGENES; SYNECHOCOCCUS; TRANS-ACTIVATORS;

EID: 84982860066     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2016.07.007     Document Type: Article

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