CRISPR/Cas9-mediated gene knockout screens and target identification via whole-genome sequencing uncover host genes required for picornavirus infection

Journal of Biological Chemistry

Volumn 292, Issue 25, 2017, Pages 10664-10671

  Kim, Heon Seok ^a,b   Lee, Kyungjin ^c   Bae, Sangsu ^d   Park, Jeongbin ^d   Lee, Chong Kyo ^c   Kim, Meehyein ^c   Kim, Eunji ^e   Kim, Minju ^e   Kim, Seokjoong ^e   Kim, Chonsaeng ^c   Kim, Jin Soo ^a,b  

^a Institute for Basic Science   (South Korea)

^b Seoul National University   (South Korea)

^c Korea Research Institute of Chemical Technology   (South Korea)

^d Hanyang University   (South Korea)

^e ToolGen Inc   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOLOGY; DNA; LIBRARIES; OLIGONUCLEOTIDES; VIRUSES;

CELL SURVIVAL; CYTOTOXIC DRUGS; EXPRESSION CASSETTE; GENETIC SCREENS; STATISTICAL ESTIMATION; TARGET IDENTIFICATION; VIRAL INFECTIONS; WHOLE GENOME SEQUENCING;

GENES;

CASPASE 9; GUIDE RNA; OLIGONUCLEOTIDE;

AGPAT3 GENE; ARTICLE; CD163 GENE; CELL METABOLISM; CELL SURVIVAL; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; DHRS12 GENE; DNA ISOLATION; ENTEROVIRUS; FUZ GENE; GENE; GENE IDENTIFICATION; GENE INACTIVATION; GENE LIBRARY; GENE MUTATION; GENE SEQUENCE; GENE TARGETING; GENETIC TRANSFECTION; GLI2 GENE; HPSE GENE; HUMAN; HUMAN CELL; INPP4B GENE; KLF16 GENE; LPGAT1 GENE; NONHUMAN; NTMT1 GENE; PARM1 GENE; PICORNAVIRUS INFECTION; POLIOMYELITIS VIRUS; PRIORITY JOURNAL; PROTEIN BLOOD LEVEL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; PRSS58 GENE; PVR GENE; RPGRIP1 GENE; SCARA5 GENE; SETD6 GENE; SIRT3 GENE; SMARCA5 GENE; THOC1 GENE; ULK2 GENE; WHOLE GENOME SEQUENCING; WISP3 GENE; GENE SILENCING; GENETICS; GENOME-WIDE ASSOCIATION STUDY; HELA CELL LINE; HIGH THROUGHPUT SEQUENCING; HUMAN GENOME; METABOLISM; POLIOMYELITIS;

CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; GENE KNOCKDOWN TECHNIQUES; GENOME, HUMAN; GENOME-WIDE ASSOCIATION STUDY; HELA CELLS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; POLIOMYELITIS; POLIOVIRUS;

EID: 85021640622     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M117.782425     Document Type: Article

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