Systematic testing of enzyme perturbation sensitivities via graded dCas9 modulation in Saccharomyces cerevisiae

Metabolic Engineering

Volumn 40, Issue , 2017, Pages 14-22

  Deaner, Matthew ^a   Alper, Hal S ^a,b  

^a The University of Texas at Austin   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

CRISPR Cas9; Gene regulation; Metabolic engineering

Indexed keywords

BIOCHEMISTRY; ENZYMES; GENE EXPRESSION; GENES; ITERATIVE METHODS; METABOLIC ENGINEERING; METABOLISM; NUCLEIC ACIDS; TRANSCRIPTION; YEAST;

CRISPR-CAS9; GENOME SCALE METABOLIC MODEL; GLYCEROL BIOSYNTHESIS; METABOLIC PATHWAYS; PENTOSE PHOSPHATE PATHWAY; RATE-LIMITING STEPS; SYSTEMATIC TESTING; XYLOSE CATABOLISMS;

GENE EXPRESSION REGULATION;

DCAS9 ENDONUCLEASE; ENDONUCLEASE; GLYCEROL; MESSENGER RNA; PENTOSE PHOSPHATE; UNCLASSIFIED DRUG; XYLOSE; XYLOSE ISOMERASE; XYLULOSE; ENZYME; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; BACTERIAL GROWTH; BIOSYNTHESIS; CATABOLISM; CONTROLLED STUDY; DNA SYNTHESIS; ENZYME REGULATION; ESCHERICHIA COLI; FLOW CYTOMETRY; GENE CONTROL; GENE DELETION; GENE EXPRESSION; GENETIC BACKGROUND; GENETIC ENGINEERING; METABOLIC ENGINEERING; METABOLISM; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PHENOTYPE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE; TATA BOX; CRISPR CAS SYSTEM; ENZYME ACTIVATION; GENE EXPRESSION REGULATION; GENETICS; METABOLIC FLUX ANALYSIS; PHYSIOLOGY; PROCEDURES; SENSITIVITY AND SPECIFICITY;

BIOSYNTHETIC PATHWAYS; CRISPR-CAS SYSTEMS; ENZYME ACTIVATION; ENZYMES; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; METABOLIC ENGINEERING; METABOLIC FLUX ANALYSIS; PENTOSE PHOSPHATE PATHWAY; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SENSITIVITY AND SPECIFICITY;

EID: 85013830067     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2017.01.012     Document Type: Article

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