Quantitative CRISPR interference screens in yeast identify chemical-genetic interactions and new rules for guide RNA design

Genome Biology

Volumn 17, Issue 1, 2016, Pages

  Smith, Justin D ^a   Suresh, Sundari ^a   Schlecht, Ulrich ^a   Wu, Manhong ^a   Wagih, Omar ^b   Peltz, Gary ^a   Davis, Ronald W ^a   Steinmetz, Lars M ^a,c   Parts, Leopold ^a,c,d   St Onge, Robert P ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b EUROPEAN BIOINFORMATICS INSTITUTE   (United Kingdom)

^c EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^d WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

Author keywords

Chemical genetic interactions; CRISPRi; DCas9; Nucleosome; Saccharomyces cerevisiae; Yeast

Indexed keywords

FLUCONAZOLE; GUIDE RNA; NUCLEASE; PROTEIN CAS9; UNCLASSIFIED DRUG; CHROMATIN; ERG25 PROTEIN, S CEREVISIAE; MIXED FUNCTION OXIDASE; NUCLEOSOME; SACCHAROMYCES CEREVISIAE PROTEIN;

ARTICLE; CHEMICAL GENETIC INTERACTION; CHROMATIN; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONTROLLED STUDY; ERG25 GENE; GENE; GENE INTERACTION; GENE LOCUS; GENE REPRESSION; HUMAN; HUMAN CELL; MOLECULAR GENETICS; PLASMID; QUANTITATIVE ANALYSIS; RNA ANALYSIS; RNA SEQUENCE; TRANSCRIPTION INITIATION SITE; YEAST; CRISPR CAS SYSTEM; FUNGAL GENOME; GENETICS; NUCLEOSOME; NUCLEOTIDE SEQUENCE; SACCHAROMYCES CEREVISIAE;

BASE SEQUENCE; CHROMATIN; CRISPR-CAS SYSTEMS; GENOME, FUNGAL; HUMANS; MIXED FUNCTION OXYGENASES; NUCLEOSOMES; RNA, GUIDE; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTION INITIATION SITE;

EID: 84960094162     PISSN: 14747596     EISSN: 1474760X     Source Type: Journal    
DOI: 10.1186/s13059-016-0900-9     Document Type: Article

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