Effective gene editing by high-fidelity base editor 2 in mouse zygotes

Protein and Cell

Volumn 8, Issue 8, 2017, Pages 601-611

  Liang, Puping ^a,b   Sun, Hongwei ^a   Sun, Ying ^a   Zhang, Xiya ^a   Xie, Xiaowei ^a   Zhang, Jinran ^a   Zhang, Zhen ^a,c   Chen, Yuxi ^a   Ding, Chenhui ^a   Xiong, Yuanyan ^a   Ma, Wenbin ^a   Liu, Dan ^d   Huang, Junjiu ^a,b   Songyang, Zhou ^a,b,d  

^a SUN YAT SEN UNIVERSITY   (China)

^b SUN YAT SEN UNIVERSITY   (China)

^c Guangzhou GLAM Biotechnology Co Ltd   (China)

^d BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

base editor; high fidelity; mouse embryos; proximal site deamination; whole genome sequencing

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL MODEL; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; EMBRYO; GENE EDITING; GENE MUTATION; GENE SEQUENCE; GENOTYPE; LETTER; MALE; MOLECULAR CLONING; MOUSE; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; ZYGOTE; ANIMAL; C57BL MOUSE; CHEMISTRY; EMBRYO TRANSFER; GENETICS; GROWTH, DEVELOPMENT AND AGING; HEK293 CELL LINE; HIGH THROUGHPUT SEQUENCING; HUMAN; MAMMALIAN EMBRYO; METABOLISM; MICROINJECTION; NUCLEOTIDE SEQUENCE; PLASMID; POINT MUTATION; PROCEDURES; TRANSPLANTATION;

APOLIPOPROTEIN B MESSENGER RNA EDITING ENZYME CATALYTIC POLYPEPTIDE 1; BACTERIAL PROTEIN; CAS9 ENDONUCLEASE STREPTOCOCCUS PYOGENES; CYTIDINE; ENDONUCLEASE; GUIDE RNA; THYMIDINE;

ANIMALS; APOBEC-1 DEAMINASE; BACTERIAL PROTEINS; BASE SEQUENCE; CRISPR-CAS SYSTEMS; CYTIDINE; EMBRYO TRANSFER; EMBRYO, MAMMALIAN; ENDONUCLEASES; GENE EDITING; HEK293 CELLS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; MICE; MICE, INBRED C57BL; MICROINJECTIONS; PLASMIDS; POINT MUTATION; RNA, GUIDE; THYMIDINE; ZYGOTE;

EID: 85020202069     PISSN: 1674800X     EISSN: 16748018     Source Type: Journal    
DOI: 10.1007/s13238-017-0418-2     Document Type: Letter

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