Targeted genome editing in human cells using CRISPR/Cas nucleases and truncated guide RNAs

Methods in Enzymology

Volumn 546, Issue C, 2014, Pages 21-45

  Fu, Yanfang ^a,b   Reyon, Deepak ^a,b   Keith Joung, J ^a,b  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Cas9; CRISPR; CRISPR Cas nucleases; Genome editing; Off target effects; Off target mutations; RNA guided nucleases; RNAs; Truncated guide

Indexed keywords

GENOMIC DNA; GUIDE RNA; HETERODUPLEX; NUCLEASE; TRUNCATED GUIDE RNA; UNCLASSIFIED DRUG; CRISPR ASSOCIATED PROTEIN; ENDONUCLEASE;

AGAR GEL ELECTROPHORESIS; AMPLICON; ARTICLE; CAPILLARY ELECTROPHORESIS; CELL VIABILITY; CRISPR CAS SYSTEM; DENATURATION; DNA MODIFICATION; FLUORESCENCE MICROSCOPY; GENOME; GENOME EDITING; GENOTYPE; HUMAN; HUMAN CELL; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRACTICE GUIDELINE; PRIORITY JOURNAL; PROMOTER REGION; STREPTOCOCCUS PYOGENES; WEB BROWSER; CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; GENE TARGETING; GENETICS; HUMAN GENOME; METABOLISM; MOLECULAR GENETICS; MUTAGENESIS; NUCLEOTIDE SEQUENCE;

BASE SEQUENCE; CELL LINE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-ASSOCIATED PROTEINS; CRISPR-CAS SYSTEMS; ENDONUCLEASES; GENE TARGETING; GENOME, HUMAN; HUMANS; MOLECULAR SEQUENCE DATA; MUTAGENESIS; RNA, GUIDE;

EID: 84925407878     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-801185-0.00002-7     Document Type: Chapter

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