Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system

Scientific Reports

Volumn 4, Issue , 2014, Pages

  Inui, Masafumi ^a   Miyado, Mami ^a   Igarashi, Maki ^a   Tamano, Moe ^a   Kubo, Atsushi ^b   Yamashita, Satoshi ^a   Asahara, Hiroshi ^a,b,c,d   Fukami, Maki ^a   Takada, Shuji ^a  

^a NATIONAL RESEARCH INSTITUTE FOR CHILD HEALTH AND DEVELOPMENT   (Japan)

^b TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^d SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SUBSTITUTION; ANIMAL; ANIMAL MODEL; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; GENETIC ENGINEERING; GENETIC TRANSFECTION; GENETICS; HUMAN; MOUSE; POINT MUTATION; PROCEDURES; SITE DIRECTED MUTAGENESIS; TRANSGENIC MOUSE;

AMINO ACID SUBSTITUTION; ANIMALS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; GENETIC ENGINEERING; HUMANS; MICE; MICE, TRANSGENIC; MODELS, ANIMAL; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; TRANSFECTION;

EID: 84903185106     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep05396     Document Type: Article

References (13)

1. Mali, P. et al. RNA-Guided Human Genome Engineering via Cas9. Science 339, 823-826 (2013).
2. Cong, L. et al. Multiplex Genome Engineering Using CRISPR/Cas Systems. Science 339, 819-823 (2013).
3. Parker, K. L. & Schimmer, B. P. Steroidogenic factor 1: a key determinant of endocrine development and function. Endocrine Reviews 18, 361-377 (1997).
4. Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat. Biotechnol. 31, 822-6 (2013).
5. Ran, F. A. et al. Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity. Cell 154, 1380-9 (2013).
6. Hsu, P. D. et al. DNA targeting specificity of RNA-guided Cas9 nucleases. Nat. Biotechnol. 31, 827-832 (2013).
7. Akiyama, H. et al. The transcription factor Sox9 is degraded by the ubiquitinproteasome system and stabilized by a mutation in a ubiquitin-target site. Matrix Biol. 23, 5083-5093 (2005).
8. Lee, P. C. et al. SUMOylated SoxE factors recruit Grg4 and function as transcriptional repressors in the neural crest. J. Cell Biol. 198, 799-813 (2012).
9. Wang, H. et al. One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering. Cell 153, 910-918 (2013).
10. Elliott, B., Richardson, C., Winderbaum, J., Nickoloff, J. A. & Jasin, M. Gene conversion tracts from double-strand break repair in mammalian cells. Mol. Cell Biol. 18, 93-101 (1998).
11. Mashiko, D. et al. Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci. Rep. 3, 3355 (2013).
12. Kato, T. et al. Production of Sry knockout mouse using TALEN via oocyte injection. Sci. Rep. 3, 3136 (2013).
13. Takada, S. et al. Targeted Gene Deletion of miRNAs in Mice by TALEN System. PLoS ONE 8, e76004 (2013).