Efficient laboratory evolution of computationally designed enzymes with low starting activities using fluorescence-activated droplet sorting

Protein Engineering, Design and Selection

Volumn 29, Issue 9, 2016, Pages 355-366

  Obexer, Richard ^a   Pott, Moritz ^a   Zeymer, Cathleen ^a   Griffiths, Andrew D ^b   Hilvert, Donald ^a  

^a ETH ZURICH   (Switzerland)

^b PSL RESEARCH UNIVERSITY   (France)

Author keywords

computational enzyme design; directed evolution; fluorescence activated droplet sorting; microfluidics; retroaldolase

Indexed keywords

CATALYST ACTIVITY; DROPS; FLUORESCENCE; MICROFLUIDICS;

DIRECTED EVOLUTION; ENHANCED EFFICIENCY; INITIAL DESIGN; MICROFLUIDIC-BASED; MICROTITER PLATES; MUTANT LIBRARIES; RATE ACCELERATION; RETROALDOLASE;

ENZYMES;

FRUCTOSE BISPHOSPHATE ALDOLASE; LYSINE;

ARTICLE; CATALYST; CELL SELECTION; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME ASSAY; FLUORESCENCE ACTIVATED DROPLET SORTING; MICROFLUIDICS; PRIORITY JOURNAL; AMINO ACID SEQUENCE; CHEMISTRY; COMPUTER AIDED DESIGN; DIRECTED MOLECULAR EVOLUTION; FLOW CYTOMETRY; GENETICS; METABOLISM; MUTATION; PROCEDURES;

AMINO ACID SEQUENCE; COMPUTER-AIDED DESIGN; DIRECTED MOLECULAR EVOLUTION; FLOW CYTOMETRY; FRUCTOSE-BISPHOSPHATE ALDOLASE; MUTATION;

EID: 85010332746     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzw032     Document Type: Article

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