Exploration of alternate catalytic mechanisms and optimization strategies for retroaldolase design

Journal of Molecular Biology

Volumn 426, Issue 1, 2014, Pages 256-271

  Bjelic, Sinisa ^a   Kipnis, Yakov ^a   Wang, Ling ^a   Pianowski, Zbigniew ^b   Vorobiev, Sergey ^c   Su, Min ^c   Seetharaman, Jayaraman ^c   Xiao, Rong ^d   Kornhaber, Gregory ^d,e,f   Hunt, John F ^c   Tong, Liang ^c   Hilvert, Donald ^b   Baker, David ^a,g  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^b ETH ZURICH   (Switzerland)

^c Och Spine at New York Presbyterian Hospitals   (United States)

^d RUTGERS UNIVERSITY   (United States)

^e RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

^f Northeast Structural Genomics Consortium   (United States)

^g UNIVERSITY OF WASHINGTON   (United States)

Author keywords

enzyme design; enzyme optimization; protein engineering; retroaldolase

Indexed keywords

ACETONE; ALDEHYDE DERIVATIVE; CATALYTIC ANTIBODY; GLUTAMIC ACID; LYSINE; SCHIFF BASE; WATER; FRUCTOSE BISPHOSPHATE ALDOLASE; PHOSPHATE;

ACCELERATION; ARTICLE; CATALYSIS; ENZYME ACTIVE SITE; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; REACTION ANALYSIS; REACTION OPTIMIZATION; SITE DIRECTED MUTAGENESIS; SOLUBILITY; X RAY; YEAST; AMINO ACID SUBSTITUTION; CATALYST; COMPUTER ANALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STRUCTURE; MOLECULAR MECHANICS; MUTAGENESIS; STEADY STATE;

EID: 84890857259     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2013.10.012     Document Type: Article

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