Optimization of the in-silico-designed Kemp eliminase KE70 by computational design and directed evolution

Journal of Molecular Biology

Volumn 407, Issue 3, 2011, Pages 391-412

  Khersonsky, Olga ^a   Röthlisberger, Daniela ^b   Wollacott, Andrew M ^b   Murphy, Paul ^b   Dym, Orly ^a   Albeck, Shira ^a   Kiss, Gert ^c   Houk, K N ^c   Baker, David ^b,d   Tawfik, Dan S ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^b UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF WASHINGTON   (United States)

Author keywords

computational protein design; directed evolution; enzymatic catalysis

Indexed keywords

ASPARTIC ACID; HISTIDINE; KEMP ELIMINASE KE70; LYASE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; COMBINATORIAL LIBRARY; COMPUTER MODEL; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME CONFORMATION; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; MOLECULAR DYNAMICS; MUTATION; PH; PKA; PREDICTION; PRIORITY JOURNAL; STATIC ELECTRICITY; STRUCTURE ANALYSIS;

EID: 79952437832     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2011.01.041     Document Type: Article

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