Robust design and optimization of retroaldol enzymes

Protein Science

Volumn 21, Issue 5, 2012, Pages 717-726

  Althoff, Eric A ^a,b   Wang, Ling ^a   Jiang, Lin ^a,c   Giger, Lars ^d   Lassila, Jonathan K ^e   Wang, Zhizhi ^a   Smith, Matthew ^a   Hari, Sanjay ^a   Kast, Peter ^d   Herschlag, Daniel ^e   Hilvert, Donald ^d   Baker, David ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^b Arzeda Corporation   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d ETH ZURICH   (Switzerland)

^e STANFORD UNIVERSITY   (United States)

Author keywords

Aldolase; Computational enzyme design; Computational protein design; Directed evolution; Enzyme; Enzyme engineering; Rational design

Indexed keywords

FRUCTOSE BISPHOSPHATE ALDOLASE; LYSINE; RETROALDOLASE; SCAFFOLD PROTEIN; UNCLASSIFIED DRUG; WATER;

ARTICLE; CATALYST; DIRECTED MOLECULAR EVOLUTION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STRUCTURE; HYDROPHOBICITY; PHASE TRANSITION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN MOTIF; SITE DIRECTED MUTAGENESIS;

ALDEHYDE-LYASES; CATALYTIC DOMAIN; DIRECTED MOLECULAR EVOLUTION; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

EID: 84860115640     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.2059     Document Type: Article

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