Ultra-high-throughput screening method for the directed evolution of glucose oxidase

Chemistry and Biology

Volumn 21, Issue 3, 2014, Pages 414-421

  Ostafe, Raluca ^a,b   Prodanovic, Radivoje ^b,c   Nazor, Jovana ^d   Fischer, Rainer ^a,b  

^a FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME   (Germany)

^b RWTH AACHEN UNIVERSITY   (Germany)

^c UNIVERSITY OF BELGRADE   (Serbia)

^d Codexis ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME VARIANT; FLUORESCENT DYE; GLUCOSE; GLUCOSE OXIDASE; OCTYL GLUCOSIDE; FUNGAL PROTEIN; HORSERADISH PEROXIDASE;

ARTICLE; CELL CLONE; DIRECTED EVOLUTION; ENZYMATIC ASSAY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE; FLOW CYTOMETRY; GENE LIBRARY; HIGH THROUGHPUT SCREENING; IN VITRO STUDY; MOLECULAR EVOLUTION; MOLECULAR LIBRARY; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; SCREENING; THERMOSTABILITY; ULTRA HIGH THROUGHPUT SCREENING; YEAST CELL; AMINO ACID SEQUENCE; ASPERGILLUS NIGER; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; ENZYMOLOGY; GENETICS; HALF LIFE TIME; METABOLISM; MOLECULAR GENETICS; PROTEIN STABILITY; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS; TEMPERATURE;

AMINO ACID SEQUENCE; ASPERGILLUS NIGER; DIRECTED MOLECULAR EVOLUTION; FLOW CYTOMETRY; FUNGAL PROTEINS; GENE LIBRARY; GLUCOSE; GLUCOSE OXIDASE; HALF-LIFE; HIGH-THROUGHPUT SCREENING ASSAYS; HORSERADISH PEROXIDASE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN STABILITY; SACCHAROMYCES CEREVISIAE; TEMPERATURE;

EID: 84897065790     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chembiol.2014.01.010     Document Type: Article

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