-
1
-
-
79951481957
-
Initial impact of the sequencing of the human genome
-
Lander, E.S. Initial impact of the sequencing of the human genome. Nature 470, 187-197 (2011).
-
(2011)
Nature
, vol.470
, pp. 187-197
-
-
Lander, E.S.1
-
2
-
-
84892607343
-
Realizing the promise of cancer predisposition genes
-
Rahman, N. Realizing the promise of cancer predisposition genes. Nature 505, 302-308 (2014).
-
(2014)
Nature
, vol.505
, pp. 302-308
-
-
Rahman, N.1
-
3
-
-
20044396570
-
Integrative analysis of the cancer transcriptome
-
Rhodes, D.R., Chinnaiyan, A.M. Integrative analysis of the cancer transcriptome. Nat. Genet. 37 (suppl.): S31-S37 (2005).
-
(2005)
Nat. Genet.
, vol.37
, pp. S31-S37
-
-
Rhodes, D.R.1
Chinnaiyan, A.M.2
-
4
-
-
26844576371
-
Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules
-
Zhang, Y. et al. Time-resolved mass spectrometry of tyrosine phosphorylation sites in the epidermal growth factor receptor signaling network reveals dynamic modules. Mol. Cell. Proteomics 4, 1240-1250 (2005).
-
(2005)
Mol. Cell. Proteomics
, vol.4
, pp. 1240-1250
-
-
Zhang, Y.1
-
5
-
-
70450242805
-
Exploring protein fitness landscapes by directed evolution
-
Romero, P.A., Arnold, F.H. Exploring protein fitness landscapes by directed evolution. Nat. Rev. Mol. Cell Biol. 10, 866-876 (2009).
-
(2009)
Nat. Rev. Mol. Cell Biol.
, vol.10
, pp. 866-876
-
-
Romero, P.A.1
Arnold, F.H.2
-
6
-
-
84924425611
-
An engineered Axl 'decoy receptor' effectively silences the Gas6-Axl signaling axis
-
Kariolis, M.S. et al. An engineered Axl 'decoy receptor' effectively silences the Gas6-Axl signaling axis. Nat. Chem. Biol. 10, 977-983 (2014).
-
(2014)
Nat. Chem. Biol.
, vol.10
, pp. 977-983
-
-
Kariolis, M.S.1
-
7
-
-
84878391705
-
Engineered thermostable fungal Cel6A and Cel7A cellobiohydrolases hydrolyze cellulose efficiently at elevated temperatures
-
Wu, I., Arnold, F.H. Engineered thermostable fungal Cel6A and Cel7A cellobiohydrolases hydrolyze cellulose efficiently at elevated temperatures. Biotechnol. Bioeng. 110, 1874-1883 (2013).
-
(2013)
Biotechnol. Bioeng.
, vol.110
, pp. 1874-1883
-
-
Wu, I.1
Arnold, F.H.2
-
8
-
-
84871556263
-
Dimerization-dependent green and yellow fluorescent proteins
-
Alford, S.C., Ding, Y., Simmen, T., Campbell, R.E. Dimerization-dependent green and yellow fluorescent proteins. ACS Synth. Biol. 1, 569-575 (2012).
-
(2012)
ACS Synth. Biol.
, vol.1
, pp. 569-575
-
-
Alford, S.C.1
Ding, Y.2
Simmen, T.3
Campbell, R.E.4
-
9
-
-
79960980943
-
A general strategy for the evolution of bond-forming enzymes using yeast display
-
Chen, I., Dorr, B.M., Liu, D.R. A general strategy for the evolution of bond-forming enzymes using yeast display. Proc. Natl. Acad. Sci. USA 108, 11399-11404 (2011).
-
(2011)
Proc. Natl. Acad. Sci. USA
, vol.108
, pp. 11399-11404
-
-
Chen, I.1
Dorr, B.M.2
Liu, D.R.3
-
10
-
-
34648832245
-
Yeast surface display for protein engineering and characterization
-
Gai, S.A., Wittrup, K.D. Yeast surface display for protein engineering and characterization. Curr. Opin. Struct. Biol. 17, 467-473 (2007).
-
(2007)
Curr. Opin. Struct. Biol.
, vol.17
, pp. 467-473
-
-
Gai, S.A.1
Wittrup, K.D.2
-
11
-
-
3242760800
-
High-affinity human antibodies from phage-displayed synthetic Fab libraries with a single framework scaffold
-
Lee, C.V. et al. High-affinity human antibodies from phage-displayed synthetic Fab libraries with a single framework scaffold. J. Mol. Biol. 340, 1073-1093 (2004).
-
(2004)
J. Mol. Biol.
, vol.340
, pp. 1073-1093
-
-
Lee, C.V.1
-
12
-
-
84856289656
-
High-throughput screening: The hits and leads of drug discovery
-
Martis, E. High-throughput screening: the hits and leads of drug discovery. J. Appl. Pharm. Sci. 01, 02-10 (2011).
-
(2011)
J. Appl. Pharm. Sci.
, vol.1
, pp. 02-10
-
-
Martis, E.1
-
13
-
-
77953718211
-
Sequence space and the ongoing expansion of the protein universe
-
Povolotskaya, I.S., Kondrashov, F.A. Sequence space and the ongoing expansion of the protein universe. Nature 465, 922-926 (2010).
-
(2010)
Nature
, vol.465
, pp. 922-926
-
-
Povolotskaya, I.S.1
Kondrashov, F.A.2
-
14
-
-
77749270677
-
Ultrahigh-throughput screening in drop-based microfluidics for directed evolution
-
Agresti, J.J. et al. Ultrahigh-throughput screening in drop-based microfluidics for directed evolution. Proc. Natl. Acad. Sci. USA 107, 4004-4009 (2010).
-
(2010)
Proc. Natl. Acad. Sci. USA
, vol.107
, pp. 4004-4009
-
-
Agresti, J.J.1
-
15
-
-
84897065790
-
Ultra-high-throughput screening method for the directed evolution of glucose oxidase
-
Ostafe, R., Prodanovic, R., Nazor, J., Fischer, R. Ultra-high-throughput screening method for the directed evolution of glucose oxidase. Chem. Biol. 21, 414-421 (2014).
-
(2014)
Chem. Biol.
, vol.21
, pp. 414-421
-
-
Ostafe, R.1
Prodanovic, R.2
Nazor, J.3
Fischer, R.4
-
16
-
-
84900653348
-
One in a million: Flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution
-
Zinchenko, A. et al. One in a million: flow cytometric sorting of single cell-lysate assays in monodisperse picolitre double emulsion droplets for directed evolution. Anal. Chem. 86, 2526-2533 (2014).
-
(2014)
Anal. Chem.
, vol.86
, pp. 2526-2533
-
-
Zinchenko, A.1
-
17
-
-
84906549486
-
Evolution of enzyme catalysts caged in biomimetic gel-shell beads
-
Fischlechner, M. et al. Evolution of enzyme catalysts caged in biomimetic gel-shell beads. Nat. Chem. 6, 791-796 (2014).
-
(2014)
Nat. Chem.
, vol.6
, pp. 791-796
-
-
Fischlechner, M.1
-
18
-
-
84931291559
-
Dissecting enzyme function with microfluidic-based deep mutational scanning
-
Romero, P.A., Tran, T.M., Abate, A.R. Dissecting enzyme function with microfluidic-based deep mutational scanning. Proc. Natl. Acad. Sci. USA 112, 7159-7164 (2015).
-
(2015)
Proc. Natl. Acad. Sci. USA
, vol.112
, pp. 7159-7164
-
-
Romero, P.A.1
Tran, T.M.2
Abate, A.R.3
-
19
-
-
4344608786
-
GigaMatrix: An ultra high-throughput tool for accessing biodiversity
-
Lafferty, M., Dycaico, M.J. GigaMatrix: an ultra high-throughput tool for accessing biodiversity. J. Lab. Autom. 9, 200-208 (2004).
-
(2004)
J. Lab. Autom.
, vol.9
, pp. 200-208
-
-
Lafferty, M.1
Dycaico, M.J.2
-
20
-
-
24644467159
-
Construction of novel single-cell screening system using a yeast cell chip for nano-sized modified-protein-displaying libraries
-
Fukuda, T., Shiraga, S., Kato, M., Yamamura, S. Construction of novel single-cell screening system using a yeast cell chip for nano-sized modified-protein-displaying libraries. NanoBiotechnology 1, 105-111 (2005).
-
(2005)
NanoBiotechnology
, vol.1
, pp. 105-111
-
-
Fukuda, T.1
Shiraga, S.2
Kato, M.3
Yamamura, S.4
-
21
-
-
69949143453
-
Mechanistic aspects of horseradish peroxidase elucidated through single-molecule studies
-
Gorris, H.H., Walt, D.R. Mechanistic aspects of horseradish peroxidase elucidated through single-molecule studies. J. Am. Chem. Soc. 131, 6277-6282 (2009).
-
(2009)
J. Am. Chem. Soc.
, vol.131
, pp. 6277-6282
-
-
Gorris, H.H.1
Walt, D.R.2
-
22
-
-
84899494694
-
A single molecule perspective on the functional diversity of in vitro evolved-glucuronidase
-
Liebherr, R.B., Renner, M., Gorris, H.H. A single molecule perspective on the functional diversity of in vitro evolved-glucuronidase. J. Am. Chem. Soc. 136, 5949-5955 (2014).
-
(2014)
J. Am. Chem. Soc.
, vol.136
, pp. 5949-5955
-
-
Liebherr, R.B.1
Renner, M.2
Gorris, H.H.3
-
23
-
-
77953246142
-
Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations
-
Rissin, D.M. et al. Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations. Nat. Biotechnol. 28, 595-599 (2010).
-
(2010)
Nat. Biotechnol.
, vol.28
, pp. 595-599
-
-
Rissin, D.M.1
-
24
-
-
33745072443
-
A microengraving method for rapid selection of single cells producing antigen-specific antibodies
-
Love, J.C., Ronan, J.L., Grotenbreg, G.M., van der Veen, A.G., Ploegh, H.L. A microengraving method for rapid selection of single cells producing antigen-specific antibodies. Nat. Biotechnol. 24, 703-707 (2006).
-
(2006)
Nat. Biotechnol.
, vol.24
, pp. 703-707
-
-
Love, J.C.1
Ronan, J.L.2
Grotenbreg, G.M.3
Van Der Veen, A.G.4
Ploegh, H.L.5
-
25
-
-
84922572327
-
Exploiting highly ordered subnanoliter volume microcapillaries as microtools for the analysis of antibody producing cells
-
Fitzgerald, V. et al. Exploiting highly ordered subnanoliter volume microcapillaries as microtools for the analysis of antibody producing cells. Anal. Chem 87, 997-103 (2015).
-
(2015)
Anal. Chem
, vol.87
, pp. 997-1103
-
-
Fitzgerald, V.1
-
26
-
-
37249018503
-
Intuitive image-based cell sorting using optofluidic cell sorting
-
Kovac, J.R., Voldman, J. Intuitive, image-based cell sorting using optofluidic cell sorting. Anal. Chem. 79, 9321-9330 (2007).
-
(2007)
Anal. Chem.
, vol.79
, pp. 9321-9330
-
-
Kovac, J.R.1
Voldman, J.2
-
27
-
-
84889577845
-
Micropallet arrays for the capture, isolation and culture of circulating tumor cells from whole blood of mice engrafted with primary human pancreatic adenocarcinoma
-
Gach, P.C., Attayek, P.J., Whittlesey, R.L., Yeh, J.J., Allbritton, N.L. Micropallet arrays for the capture, isolation and culture of circulating tumor cells from whole blood of mice engrafted with primary human pancreatic adenocarcinoma. Biosens. Bioelectron. 54, 476-483 (2014).
-
(2014)
Biosens. Bioelectron.
, vol.54
, pp. 476-483
-
-
Gach, P.C.1
Attayek, P.J.2
Whittlesey, R.L.3
Yeh, J.J.4
Allbritton, N.L.5
-
28
-
-
67649612733
-
Accuracy and precision in quantitative fluorescence microscopy
-
Waters, J.C. Accuracy and precision in quantitative fluorescence microscopy. J. Cell Biol. 185, 1135-1148 (2009).
-
(2009)
J. Cell Biol.
, vol.185
, pp. 1135-1148
-
-
Waters, J.C.1
-
29
-
-
42649140235
-
Immunobiology of the TAM receptors
-
Lemke, G., Rothlin, C.V. Immunobiology of the TAM receptors. Nat. Rev. Immunol. 8, 327-336 (2008).
-
(2008)
Nat. Rev. Immunol.
, vol.8
, pp. 327-336
-
-
Lemke, G.1
Rothlin, C.V.2
-
30
-
-
84922481128
-
The TAM family: Phosphatidylserine-sensing receptor tyrosine kinases gone awry in cancer
-
Graham, D.K., DeRyckere, D., Davies, K.D., Earp, H.S. The TAM family: phosphatidylserine-sensing receptor tyrosine kinases gone awry in cancer. Nat. Rev. Cancer 14, 769-785 (2014).
-
(2014)
Nat. Rev. Cancer
, vol.14
, pp. 769-785
-
-
Graham, D.K.1
DeRyckere, D.2
Davies, K.D.3
Earp, H.S.4
-
31
-
-
1842609526
-
Phage-displayed antibody libraries of synthetic heavy chain complementarity determining regions
-
Sidhu, S.S. et al. Phage-displayed antibody libraries of synthetic heavy chain complementarity determining regions. J. Mol. Biol. 338, 299-310 (2004).
-
(2004)
J. Mol. Biol.
, vol.338
, pp. 299-310
-
-
Sidhu, S.S.1
-
32
-
-
84897136274
-
Engineering and characterizing monomeric fluorescent proteins for live-cell imaging applications
-
Ai, H.-W., Baird, M.A., Shen, Y., Davidson, M.W., Campbell, R.E. Engineering and characterizing monomeric fluorescent proteins for live-cell imaging applications. Nat. Protoc. 9, 910-928 (2014).
-
(2014)
Nat. Protoc.
, vol.9
, pp. 910-928
-
-
Ai, H.-W.1
Baird, M.A.2
Shen, Y.3
Davidson, M.W.4
Campbell, R.E.5
-
33
-
-
11144267737
-
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. Red fluorescent protein
-
Shaner, N.C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22, 1567-1572 (2004).
-
(2004)
Nat. Biotechnol.
, vol.22
, pp. 1567-1572
-
-
Shaner, N.C.1
-
34
-
-
84858964964
-
A fluorogenic red fluorescent protein heterodimer
-
Alford, S.C., Abdelfattah, A.S., Ding, Y., Campbell, R.E. A fluorogenic red fluorescent protein heterodimer. Chem. Biol. 19, 353-360 (2012).
-
(2012)
Chem. Biol.
, vol.19
, pp. 353-360
-
-
Alford, S.C.1
Abdelfattah, A.S.2
Ding, Y.3
Campbell, R.E.4
-
35
-
-
84923845791
-
Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange
-
Ding, Y. et al. Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange. Nat. Methods 12, 195-198 (2015).
-
(2015)
Nat. Methods
, vol.12
, pp. 195-198
-
-
Ding, Y.1
-
36
-
-
0026773209
-
Structure and mechanism of alkaline phosphatase
-
Coleman, J. Structure and mechanism of alkaline phosphatase. Annu. Rev. Biophys. Biomol. Struct. 21, 441-483 (1992).
-
(1992)
Annu. Rev. Biophys. Biomol. Struct.
, vol.21
, pp. 441-483
-
-
Coleman, J.1
-
37
-
-
0035873714
-
Functional interrelationships in the alkaline phosphatase superfamily: Phosphodiesterase activity of Escherichia coli alkaline phosphatase
-
O'Brien, P.J., Herschlag, D. Functional interrelationships in the alkaline phosphatase superfamily: phosphodiesterase activity of Escherichia coli alkaline phosphatase. Biochemistry 40, 5691-5699 (2001).
-
(2001)
Biochemistry
, vol.40
, pp. 5691-5699
-
-
O'Brien, P.J.1
Herschlag, D.2
-
38
-
-
84355161649
-
Removing allosteric feedback inhibition of tomato 4-coumarate:CoA ligase by directed evolution
-
Alberstein, M., Eisenstein, M., Abeliovich, H. Removing allosteric feedback inhibition of tomato 4-coumarate:CoA ligase by directed evolution. Plant J. 69, 57-69 (2012).
-
(2012)
Plant J
, vol.69
, pp. 57-69
-
-
Alberstein, M.1
Eisenstein, M.2
Abeliovich, H.3
-
39
-
-
84864590169
-
Rational engineering of enzyme allosteric regulation through sequence evolution analysis
-
Yang, J.-S., Seo, S.W., Jang, S., Jung, G.Y., Kim, S. Rational engineering of enzyme allosteric regulation through sequence evolution analysis. PLoS Comput. Biol. 8, e1002612 (2012).
-
(2012)
PLoS Comput. Biol.
, vol.8
, pp. e1002612
-
-
Yang, J.-S.1
Seo, S.W.2
Jang, S.3
Jung, G.Y.4
Kim, S.5
-
40
-
-
77955550139
-
Engineering of a fungal-galactosidase to remove product inhibition by galactose
-
Hu, X., Robin, S., O'Connell, S., Walsh, G., Wall, J.G. Engineering of a fungal-galactosidase to remove product inhibition by galactose. Appl. Microbiol. Biotechnol. 87, 1773-1782 (2010).
-
(2010)
Appl. Microbiol. Biotechnol.
, vol.87
, pp. 1773-1782
-
-
Hu, X.1
Robin, S.2
O'Connell, S.3
Walsh, G.4
Wall, J.G.5
-
41
-
-
84872495336
-
Olefin cyclopropanation via carbene transfer catalyzed by engineered cytochrome P450 enzymes
-
Coelho, P.S., Brustad, E.M., Kannan, A., Arnold, F.H. Olefin cyclopropanation via carbene transfer catalyzed by engineered cytochrome P450 enzymes. Science 339, 307-310 (2013).
-
(2013)
Science
, vol.339
, pp. 307-310
-
-
Coelho, P.S.1
Brustad, E.M.2
Kannan, A.3
Arnold, F.H.4
-
42
-
-
0038440614
-
Selective cell targeting with light-absorbing microparticles and nanoparticles
-
Pitsillides, C.M., Joe, E.K., Wei, X., Anderson, R.R., Lin, C.P. Selective cell targeting with light-absorbing microparticles and nanoparticles. Biophys. J. 84, 4023-4032 (2003).
-
(2003)
Biophys. J.
, vol.84
, pp. 4023-4032
-
-
Pitsillides, C.M.1
Joe, E.K.2
Wei, X.3
Anderson, R.R.4
Lin, C.P.5
-
43
-
-
84928928784
-
Massively parallel delivery of large cargo into mammalian cells with light pulses
-
Wu, Y.-C. et al. Massively parallel delivery of large cargo into mammalian cells with light pulses. Nat. Methods 12, 439-444 (2015).
-
(2015)
Nat. Methods
, vol.12
, pp. 439-444
-
-
Wu, Y.-C.1
-
44
-
-
0018306059
-
A threshold selection method from gray-level histograms
-
Otsu, N. A threshold selection method from gray-level histograms. IEEE SMC 9, 62-66 (1979).
-
(1979)
IEEE SMC
, vol.9
, pp. 62-66
-
-
Otsu, N.1
-
45
-
-
84934439680
-
Yeast surface display for antibody isolation: Library construction, library screening, and affinity maturation
-
Van Deventer, J.A., Wittrup, K.D. Yeast surface display for antibody isolation: library construction, library screening, and affinity maturation. Methods Mol. Biol. 1131, 151-181 (2014).
-
(2014)
Methods Mol. Biol.
, vol.1131
, pp. 151-181
-
-
Van Deventer, J.A.1
Wittrup, K.D.2
-
46
-
-
34247176809
-
Isolating and engineering human antibodies using yeast surface display
-
Chao, G. et al. Isolating and engineering human antibodies using yeast surface display. Nat. Protoc. 1, 755-768 (2006).
-
(2006)
Nat. Protoc.
, vol.1
, pp. 755-768
-
-
Chao, G.1
-
47
-
-
84877716122
-
Performance of the parametric bootstrap method in small sample interval estimates
-
Benton, D., Krishnamoorthy, K. Performance of the parametric bootstrap method in small sample interval estimates. Adv. Appl. Stat. 2, 269-285 (2002).
-
(2002)
Adv. Appl. Stat.
, vol.2
, pp. 269-285
-
-
Benton, D.1
Krishnamoorthy, K.2
-
48
-
-
84908499924
-
Probing the origins of catalytic discrimination between phosphate and sulfate monoester hydrolysis: Comparative analysis of alkaline phosphatase and protein tyrosine phosphatases
-
Andrews, L.D., Zalatan, J.G., Herschlag, D. Probing the origins of catalytic discrimination between phosphate and sulfate monoester hydrolysis: comparative analysis of alkaline phosphatase and protein tyrosine phosphatases. Biochemistry 53, 6811-6819 (2014).
-
(2014)
Biochemistry
, vol.53
, pp. 6811-6819
-
-
Andrews, L.D.1
Zalatan, J.G.2
Herschlag, D.3
-
49
-
-
0028098798
-
Direct real-time measurement of rapid inorganic phosphate release using a novel fluorescent probe and its application to actomyosin subfragment 1 ATPase
-
Brune, M., Hunter, J.L., Corrie, J.E.T., Webb, M.R. Direct, real-time measurement of rapid inorganic phosphate release using a novel fluorescent probe and its application to actomyosin subfragment 1 ATPase. Biochemistry 33, 8262-8271 (1994).
-
(1994)
Biochemistry
, vol.33
, pp. 8262-8271
-
-
Brune, M.1
Hunter, J.L.2
Corrie, J.E.T.3
Webb, M.R.4
-
50
-
-
56949087215
-
Comparative enzymology in the alkaline phosphatase superfamily to determine the catalytic role of an active-site metal ion
-
Zalatan, J.G., Fenn, T.D., Herschlag, D. Comparative enzymology in the alkaline phosphatase superfamily to determine the catalytic role of an active-site metal ion. J. Mol. Biol. 384, 1174-1189 (2008).
-
(2008)
J. Mol. Biol.
, vol.384
, pp. 1174-1189
-
-
Zalatan, J.G.1
Fenn, T.D.2
Herschlag, D.3
|