Impact of scaffold rigidity on the design and evolution of an artificial Diels-Alderase

Proceedings of the National Academy of Sciences of the United States of America

Volumn 111, Issue 22, 2014, Pages 8013-8018

  Preiswerk, Nathalie ^a   Beck, Tobias ^a   Schulz, Jessica D ^a   Milovník, Peter ^a,e   Mayer, Clemens ^a   Siegel, Justin B ^b,c,d   Baker, David ^b   Hilvert, Donald ^a  

^a ETH ZURICH   (Switzerland)

^b UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e AbCheck   (Czech Republic)

Author keywords

Biocatalysis; Computational enzyme design; Diels Alder reaction; Enzyme mechanism; Laboratory evolution

Indexed keywords

DIELS ALDERASE; ENZYME; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CATALYST; CHEMICAL REACTION; CRYSTAL STRUCTURE; CYCLOADDITION; DIELS ALDER REACTION; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME INHIBITION; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN STRUCTURE; RIGIDITY; STEREOCHEMISTRY; X RAY CRYSTALLOGRAPHY;

BIOCATALYSIS; COMPUTATIONAL ENZYME DESIGN; DIELS-ALDER REACTION; ENZYME MECHANISM; LABORATORY EVOLUTION;

ACRYLAMIDES; BUTADIENES; CATALYSIS; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; CYCLOADDITION REACTION; ENZYME ACTIVATION; ENZYMES; EVOLUTION, CHEMICAL; KINETICS; MODELS, CHEMICAL; SUBSTRATE SPECIFICITY;

EID: 84901855811     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1401073111     Document Type: Article

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