Mining Enzyme Diversity of Transcriptome Libraries through DNA Synthesis for Benzylisoquinoline Alkaloid Pathway Optimization in Yeast

ACS Synthetic Biology

Volumn 5, Issue 12, 2016, Pages 1505-1518

  Narcross, Lauren ^b   Bourgeois, Leanne ^b   Fossati, Elena ^a   Burton, Euan ^b   Martin, Vincent J J ^b  

^a Concordia University ^*

^b Gina Cody School of Engineering and Computer Science   (Canada)

Author keywords

benzylisoquinoline alkaloids; dihydrosanguinarine; pathway optimization; Saccharomyces cerevisiae; synthetic DNA; transcriptome mining

Indexed keywords

ALKALOID; AMINO ACID; BENZYLISOQUINOLINE DERIVATIVE; CYTOCHROME P450; DNA; ENZYME; METHYLTRANSFERASE; STYLOPINE; TETRAHYDROPAPAVEROLINE; TRANSCRIPTOME; BENZOPHENANTHRIDINE DERIVATIVE; BERBERINE DERIVATIVE; DIHYDROSANGUINARINE; ISOQUINOLINE DERIVATIVE; S-ADENOSYL-L-METHIONINE COCLAURINE N-METHYLTRANSFERASE;

ARTICLE; BIOSYNTHESIS; CODON; COMPARATIVE STUDY; CONTROLLED STUDY; DNA SYNTHESIS; ENZYME ACTIVITY; EXPRESSION VECTOR; FLUORESCENCE; FUNGAL CELL CULTURE; GENE EXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HOMOLOGOUS RECOMBINATION; NONHUMAN; PHYLOGENETIC TREE; PRIORITY JOURNAL; REACTION ANALYSIS; SUBSTRATE CONCENTRATION; SUPERNATANT; GENE LIBRARY; GENETICS; METABOLISM; PHYLOGENY; SACCHAROMYCES CEREVISIAE;

BENZOPHENANTHRIDINES; BENZYLISOQUINOLINES; BERBERINE ALKALOIDS; CYTOCHROME P-450 ENZYME SYSTEM; DNA; ENZYMES; GENE LIBRARY; ISOQUINOLINES; METHYLTRANSFERASES; PHYLOGENY; SACCHAROMYCES CEREVISIAE; TETRAHYDROPAPAVEROLINE; TRANSCRIPTOME;

EID: 85006341508     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.6b00119     Document Type: Article

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