Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells

Nature Protocols

Volumn 10, Issue 11, 2015, Pages 1842-1859

  Liu, Jia ^a   Gaj, Thomas ^b,f   Yang, Yifeng ^a   Wang, Nan ^a   Shui, Sailan ^a   Kim, Sojung ^c   Kanchiswamy, Chidananda Nagamangala ^d   Kim, Jin Soo ^c,e   Barbas, Carlos F ^b  

^a SHANGHAITECH UNIVERSITY   (China)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

^c Institute for Basic Science   (South Korea)

^d RESEARCH AND INNOVATION CENTRE   (Italy)

^e Seoul National University   (South Korea)

^f UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; CHEMOKINE RECEPTOR CCR5; NUCLEASE; TALEN PROTEIN; UNCLASSIFIED DRUG; RECOMBINASE;

ARTICLE; CCR5 GENE; CELL MEMBRANE PERMEABILITY; CLINICAL PROTOCOL; DNA MODIFICATION; EMBRYONIC STEM CELL; GENETIC ENGINEERING; GENOME EDITING; HUMAN; INTERNALIZATION; MEDICAL RESEARCH; PRIMARY CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; CELL CULTURE; GENE TARGETING; METABOLISM; MOLECULAR BIOLOGY; PROCEDURES; PROTEIN TRANSPORT; STEM CELL;

CELLS, CULTURED; GENE TARGETING; HUMANS; MOLECULAR BIOLOGY; PROTEIN TRANSPORT; RECOMBINASES; STEM CELLS;

EID: 84965190468     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2015.117     Document Type: Article

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