-
1
-
-
84873729095
-
Multiplex genome engineering using CRISPR/Cas systems
-
Cong, L. et al. Multiplex genome engineering using CRISPR/Cas systems. Science 339, 819-823 (2013).
-
(2013)
Science
, vol.339
, pp. 819-823
-
-
Cong, L.1
-
2
-
-
84880570576
-
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
-
Fu, Y. et al. High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol 31, 822-826 (2013).
-
(2013)
Nat Biotechnol
, vol.31
, pp. 822-826
-
-
Fu, Y.1
-
3
-
-
84883819602
-
Heritable gene targeting in the mouse and rat using a CRISPR-Cas system
-
Li, D. et al. Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nat Biotechnol 31, 681-683 (2013).
-
(2013)
Nat Biotechnol
, vol.31
, pp. 681-683
-
-
Li, D.1
-
4
-
-
84913594397
-
Genome editing. The new frontier of genome engineering with CRISPR-Cas9
-
Doudna, J. A. & Charpentier, E. Genome editing. The new frontier of genome engineering with CRISPR-Cas9. Science 346, 1258096 (2014).
-
(2014)
Science
, vol.346
-
-
Doudna, J.A.1
Charpentier, E.2
-
5
-
-
84879264708
-
ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
-
Gaj, T., Gersbach, C. A. & Barbas, C. F., 3rd. ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering. Trends Biotechnol 31, 397-405 (2013).
-
(2013)
Trends Biotechnol
, vol.31
, pp. 397-405
-
-
Gaj, T.1
Gersbach, C.A.2
Barbas, C.F.3
-
6
-
-
84884288934
-
Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity
-
Ran, F. A. et al. Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Cell 154, 1380-1389 (2013).
-
(2013)
Cell
, vol.154
, pp. 1380-1389
-
-
Ran, F.A.1
-
7
-
-
84897954175
-
Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects
-
Shen, B. et al. Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Nat Methods 11, 399-402 (2014).
-
(2014)
Nat Methods
, vol.11
, pp. 399-402
-
-
Shen, B.1
-
8
-
-
84891710947
-
Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases
-
Cho, S. W. et al. Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases. Genome Res 24, 132-141 (2014).
-
(2014)
Genome Res
, vol.24
, pp. 132-141
-
-
Cho, S.W.1
-
9
-
-
84902204289
-
Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing
-
Tsai, S. Q. et al. Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Nat Biotechnol 32, 569-576 (2014).
-
(2014)
Nat Biotechnol
, vol.32
, pp. 569-576
-
-
Tsai, S.Q.1
-
10
-
-
84937575600
-
Dimeric CRISPR RNA-Guided FokI-dCas9 Nucleases Directed by Truncated gRNAs for Highly Specific Genome Editing
-
Wyvekens, N., Topkar, V. V., Khayter, C., Joung, J. K. & Tsai, S. Q. Dimeric CRISPR RNA-Guided FokI-dCas9 Nucleases Directed by Truncated gRNAs for Highly Specific Genome Editing. Hum Gene Ther 26, 425-431 (2015).
-
(2015)
Hum Gene Ther
, vol.26
, pp. 425-431
-
-
Wyvekens, N.1
Topkar, V.V.2
Khayter, C.3
Joung, J.K.4
Tsai, S.Q.5
-
11
-
-
84902210542
-
Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification
-
Guilinger, J. P., Thompson, D. B. & Liu, D. R. Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification. Nat Biotechnol 32, 577-582 (2014).
-
(2014)
Nat Biotechnol
, vol.32
, pp. 577-582
-
-
Guilinger, J.P.1
Thompson, D.B.2
Liu, D.R.3
-
12
-
-
84925407878
-
Targeted genome editing in human cells using CRISPR/Cas nucleases and truncated guide RNAs
-
Fu, Y., Reyon, D. & Joung, J. K. Targeted genome editing in human cells using CRISPR/Cas nucleases and truncated guide RNAs. Methods Enzymol 546, 21-45 (2014).
-
(2014)
Methods Enzymol
, vol.546
, pp. 21-45
-
-
Fu, Y.1
Reyon, D.2
Joung, J.K.3
-
13
-
-
84896929630
-
Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
-
Fu, Y., Sander, J. D., Reyon, D., Cascio, V. M. & Joung, J. K. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol 32, 279-284 (2014).
-
(2014)
Nat Biotechnol
, vol.32
, pp. 279-284
-
-
Fu, Y.1
Sander, J.D.2
Reyon, D.3
Cascio, V.M.4
Joung, J.K.5
-
14
-
-
84889855901
-
One-step generation of different immunodeficient mice with multiple gene modifications by CRISPR/Cas9 mediated genome engineering
-
Zhou, J. et al. One-step generation of different immunodeficient mice with multiple gene modifications by CRISPR/Cas9 mediated genome engineering. Int J Biochem Cell Biol 46, 49-55 (2014).
-
(2014)
Int J Biochem Cell Biol
, vol.46
, pp. 49-55
-
-
Zhou, J.1
-
15
-
-
84904446988
-
A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice
-
Sakurai, T., Watanabe, S., Kamiyoshi, A., Sato, M. & Shindo, T. A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice. BMC Biotechnol 14, 69 (2014).
-
(2014)
BMC Biotechnol
, vol.14
, pp. 69
-
-
Sakurai, T.1
Watanabe, S.2
Kamiyoshi, A.3
Sato, M.4
Shindo, T.5
-
16
-
-
84905594309
-
Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system
-
Mizuno, S. et al. Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system. Mamm Genome 25, 327-334 (2014).
-
(2014)
Mamm Genome
, vol.25
, pp. 327-334
-
-
Mizuno, S.1
-
17
-
-
84897140411
-
Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering
-
Horii, T. et al. Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering. Sci Rep 4, 4513 (2014).
-
(2014)
Sci Rep
, vol.4
, pp. 4513
-
-
Horii, T.1
-
18
-
-
84930640841
-
CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells
-
Kimura, Y. et al. CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells. Sci Rep 5, 10710 (2015).
-
(2015)
Sci Rep
, vol.5
-
-
Kimura, Y.1
-
19
-
-
84923266604
-
GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
-
Tsai, S. Q. et al. GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases. Nat Biotechnol 33, 187-197 (2015).
-
(2015)
Nat Biotechnol
, vol.33
, pp. 187-197
-
-
Tsai, S.Q.1
-
20
-
-
84959123021
-
CRISPRscan: Designing highly efficient sgRNAs for CRISPR-Cas9 targeting i vivo
-
Moreno-Mateos, M. A. et al. CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting i vivo. Nat Methods 12, 982-988 (2015).
-
(2015)
Nat Methods
, vol.12
, pp. 982-988
-
-
Moreno-Mateos, M.A.1
-
21
-
-
84921540377
-
Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation
-
Doench, J. G. et al. Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Nat Biotechnol 32, 1262-1267 (2014).
-
(2014)
Nat Biotechnol
, vol.32
, pp. 1262-1267
-
-
Doench, J.G.1
-
22
-
-
84887010498
-
Genome engineering using the CRISPR-Cas9 system
-
Ran, F. A. et al. Genome engineering using the CRISPR-Cas9 system. Nat Protoc 8, 2281-2308 (2013).
-
(2013)
Nat Protoc
, vol.8
, pp. 2281-2308
-
-
Ran, F.A.1
-
23
-
-
84877707375
-
One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering
-
Wang, H. et al. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell 153, 910-918 (2013).
-
(2013)
Cell
, vol.153
, pp. 910-918
-
-
Wang, H.1
-
24
-
-
84890050551
-
Correction of a genetic disease in mouse via use of CRISPR-Cas9
-
Wu, Y. et al. Correction of a genetic disease in mouse via use of CRISPR-Cas9. Cell Stem Cell 13, 659-662 (2013).
-
(2013)
Cell Stem Cell
, vol.13
, pp. 659-662
-
-
Wu, Y.1
-
25
-
-
84902095352
-
Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells
-
Wu, X. et al. Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Nat Biotechnol 32, 670-676 (2014).
-
(2014)
Nat Biotechnol
, vol.32
, pp. 670-676
-
-
Wu, X.1
-
26
-
-
84930153818
-
Off-target mutations are rare in Cas9-modified mice
-
Iyer, V. et al. Off-target mutations are rare in Cas9-modified mice. Nat Methods 12, 479 (2015).
-
(2015)
Nat Methods
, vol.12
, pp. 479
-
-
Iyer, V.1
-
27
-
-
84897140631
-
Heritable multiplex genetic engineering in rats using CRISPR/Cas9
-
Ma, Y. et al. Heritable multiplex genetic engineering in rats using CRISPR/Cas9. PLoS One 9, e89413 (2014).
-
(2014)
PLoS One
, vol.9
-
-
Ma, Y.1
-
28
-
-
84890014631
-
Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease
-
Fujii, W., Kawasaki, K., Sugiura, K. & Naito, K. Efficient generation of large-scale genome-modified mice using gRNA and CAS9 endonuclease. Nucleic Acids Res 41, e187 (2013).
-
(2013)
Nucleic Acids Res
, vol.41
, pp. e187
-
-
Fujii, W.1
Kawasaki, K.2
Sugiura, K.3
Naito, K.4
-
29
-
-
34147177201
-
A guide to murine coagulation factor structure, function, assays, and genetic alterations
-
Emeis, J. J. et al. A guide to murine coagulation factor structure, function, assays, and genetic alterations. J Thromb Haemost 5, 670-679 (2007).
-
(2007)
J Thromb Haemost
, vol.5
, pp. 670-679
-
-
Emeis, J.J.1
-
30
-
-
0030775316
-
Mice lacking factor VII develop normally but suffer fatal perinatal bleeding
-
Rosen, E. D. et al. Mice lacking factor VII develop normally but suffer fatal perinatal bleeding. Nature 390, 290-294 (1997).
-
(1997)
Nature
, vol.390
, pp. 290-294
-
-
Rosen, E.D.1
-
31
-
-
84897413441
-
Efficient generation of genome-modified mice via offset-nicking by CRISPR/Cas system
-
Fujii, W., Onuma, A., Sugiura, K. & Naito, K. Efficient generation of genome-modified mice via offset-nicking by CRISPR/Cas system. Biochem Biophys Res Commun 445, 791-794 (2014).
-
(2014)
Biochem Biophys Res Commun
, vol.445
, pp. 791-794
-
-
Fujii, W.1
Onuma, A.2
Sugiura, K.3
Naito, K.4
-
32
-
-
84937730118
-
Efficient cryopreservation of mouse embryos by modified droplet vitrification (MDV)
-
An, L. et al. Efficient cryopreservation of mouse embryos by modified droplet vitrification (MDV). Cryobiology 71, 70-76 (2015).
-
(2015)
Cryobiology
, vol.71
, pp. 70-76
-
-
An, L.1
-
33
-
-
84905029498
-
Generating genetically modified mice using CRISPR/Cas-mediated genome engineering
-
Yang, H., Wang, H. & Jaenisch, R. Generating genetically modified mice using CRISPR/Cas-mediated genome engineering. Nat Protoc 9, 1956-1968 (2014).
-
(2014)
Nat Protoc
, vol.9
, pp. 1956-1968
-
-
Yang, H.1
Wang, H.2
Jaenisch, R.3
-
34
-
-
79951774269
-
Molecular cloning - A laboratory manual, 3rd edition
-
Chong, L. Molecular cloning - A laboratory manual, 3rd edition. Science 292, 446-446 (2001).
-
(2001)
Science
, vol.292
, pp. 446
-
-
Chong, L.1
-
35
-
-
79955947516
-
A novel missense mutation close to the charge-stabilizing system in a patient with congenital factor VII deficiency
-
Jiang, M. et al. A novel missense mutation close to the charge-stabilizing system in a patient with congenital factor VII deficiency. Blood Coagul Fibrinolysis 22, 264-270 (2011).
-
(2011)
Blood Coagul Fibrinolysis
, vol.22
, pp. 264-270
-
-
Jiang, M.1
|