A single blastocyst assay optimized for detecting CRISPR/Cas9 system-induced indel mutations in mice

BMC Biotechnology

Volumn 14, Issue , 2014, Pages

  Sakurai, Takayuki ^a   Watanabe, Satoshi ^b   Kamiyoshi, Akiko ^a   Sato, Masahiro ^c   Shindo, Takayuki ^a  

^a SHINSHU UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

^b NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

^c KAGOSHIMA UNIVERSITY   (Japan)

Author keywords

Blastocyst; CRISPR Cas9; Crude DNA solution; Gene targeting; Microinjection; Surveyor assay; T7 endonuclease I; Whole genome amplification

Indexed keywords

ASSAYS; COMMINUTION; DNA; GENES; MAMMALS; PROTEINS; SURVEYING;

BLASTOCYST; CRISPR/CAS9; DNA SOLUTIONS; GENE TARGETING; MICRO-INJECTION; T7 ENDONUCLEASE; WHOLE GENOME AMPLIFICATIONS;

POLYMERASE CHAIN REACTION;

DNA; ENDONUCLEASE; GENOMIC DNA; MESSENGER RNA; NUCLEASE; RECEPTOR ACTIVITY MODIFYING PROTEIN 2; RNA; CRISPR ASSOCIATED PROTEIN; DEOXYRIBONUCLEASE I; RAMP2 PROTEIN, MOUSE;

ANIMAL EXPERIMENT; ARTICLE; BLASTOCYST; CONTROLLED STUDY; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; DNA END JOINING REPAIR; DNA TEMPLATE; EMBRYO; EXON; FEMALE; GENE AMPLIFICATION; GENE LOCUS; GENETIC ANALYSIS; GENOME; HOMOLOGOUS RECOMBINATION; INDEL MUTATION; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; ZYGOTE; ANIMAL; C57BL MOUSE; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CYTOLOGY; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; ANIMAL TISSUE; GENE SILENCING; MICROINJECTION; NEWBORN; REPRODUCIBILITY; SINGLE CELL ANALYSIS;

MURINAE; MUS;

ANIMALS; BASE SEQUENCE; BLASTOCYST; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-ASSOCIATED PROTEINS; DEOXYRIBONUCLEASE I; DNA; GENOME; INDEL MUTATION; MICE; MICE, INBRED C57BL; MOLECULAR SEQUENCE DATA; PLASMIDS; POLYMERASE CHAIN REACTION; RECEPTOR ACTIVITY-MODIFYING PROTEIN 2; RNA, MESSENGER;

EID: 84904446988     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-14-69     Document Type: Article

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