A new approach to produce HIV-1 envelope trimers: Both cleavage and proper glycosylation are essential to generate authentic trimers

Journal of Biological Chemistry

Volumn 290, Issue 32, 2015, Pages 19780-19795

  AlSalmi, Wadad ^a   Mahalingam, Marthandan ^a   Ananthaswamy, Neeti ^a   Hamlin, Christopher ^a   Flores, Dalia ^a   Gao, Guofen ^a   Rao, Venigalla B ^a  

^a CATHOLIC UNIVERSITY OF AMERICA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; DISEASES; GLYCOSYLATION; VACCINES; VIRUSES;

BIOCHEMICAL CHARACTERIZATION; CONFORMATIONAL CHANGE; CONFORMATIONAL HETEROGENEITY; MICROHETEROGENEITY; NEUTRALIZING ANTIBODIES; PRODUCTION SYSTEM; RECOMBINANT VACCINES; SIMPLE APPROACH;

OLIGOMERS;

OLIGOMER; PROTEIN GP140; PROTEIN GP41; TRIMER; UNCLASSIFIED DRUG; VIRUS ENVELOPE PROTEIN; ANTIBODY; GLYCOPROTEIN GP 120; GLYCOPROTEIN GP 41; GP120 PROTEIN, HUMAN IMMUNODEFICIENCY VIRUS 1; GP140 ENVELOPE PROTEIN, HUMAN IMMUNODEFICIENCY VIRUS 1; GP41 PROTEIN, HUMAN IMMUNODEFICIENCY VIRUS 1; HYBRID PROTEIN; OLIGOPEPTIDE; TRP-SER- HIS-PRO-GLN-PHE-GLU-LYS; VIRUS ANTIGEN;

ANTIBODY RESPONSE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN CROSS LINKING; PROTEIN EXPRESSION; PROTEIN GLYCOSYLATION; PROTEIN INTERACTION; PROTEIN PURIFICATION; VIRUS CELL INTERACTION; VIRUS CULTURE; VIRUS ENVELOPE; VIRUS SPIKE; AMINO ACID SEQUENCE; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; GLYCOSYLATION; IMMUNOLOGY; METABOLISM; MOLECULAR GENETICS; PROTEIN DEGRADATION; PROTEIN MULTIMERIZATION; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE;

HUMAN IMMUNODEFICIENCY VIRUS 1;

AMINO ACID SEQUENCE; ANTIBODIES; ANTIGENS, VIRAL; ENV GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENE EXPRESSION; GLYCOSYLATION; HIV ENVELOPE PROTEIN GP120; HIV ENVELOPE PROTEIN GP41; HIV-1; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PROTEOLYSIS; RECOMBINANT FUSION PROTEINS;

EID: 84939128392     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M115.656611     Document Type: Article

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