Stabilization of the soluble, cleaved, trimeric form of the envelope glycoprotein complex of human immunodeficiency virus type 1

Journal of Virology

Volumn 76, Issue 17, 2002, Pages 8875-8889

  Sanders, Rogier W ^a   Vesanen, Mika ^a   Schuelke, Norbert ^a   Master, Aditi ^a   Schiffner, Linnea ^a   Kalyanaraman, Roopa ^a   Paluch, Maciej ^a   Berkhout, Ben ^a   Maddon, Paul J ^a   Olson, William C ^a   Lu, Min ^a   Moore, John P ^a  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCOPROTEIN;

ANIMAL CELL; ANTIGENICITY; ARTICLE; CELL FUSION; COMPLEX FORMATION; COVALENT BOND; DISULFIDE BOND; GEL FILTRATION CHROMATOGRAPHY; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN STABILITY; VACCINATION; VIRUS ENVELOPE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CELL LINE; DIMERIZATION; DISULFIDES; GENE PRODUCTS, ENV; HIV ENVELOPE PROTEIN GP120; HIV ENVELOPE PROTEIN GP41; HIV-1; HUMANS; SOLUBILITY;

EID: 0036333661     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.76.17.8875-8889.2002     Document Type: Article

References (106)

1. Expression and characterization of CD4-IgG2, a novel heterotetramer that neutralizes primary HIV type 1 isolates
2. The ability of an oligomeric human immunodeficiency virus type 1 (HIV-1) envelope antigen to elicit neutralizing antibodies against primary HIV-1 isolates is improved following partial deletion of the second hypervariable region
3. Oligomerization of the hydrophobic heptad repeat of gp41
4. Design of a novel peptide inhibitor of HIV fusion that disrupts the internal trimeric coiled-coil of gp41
5. A recombinant human immunodeficiency virus envelope glycoprotein complex stabilized by an intermolecular disulfide bond between the gp120 and gp41 subunits is an antigenic mimic of the trimeric virion-associated structure
6. Enhancing the proteolytic maturation of human immunodeficiency virus type 1 envelope glycoproteins
7. Enhanced immunogenicity of a human immunodeficiency virus type 1 env DNA vaccine by manipulating N-glycosylation signals. Effects of elimination of the V3 N306 glycan
8. Structure of influenza haemagglutinin at the pH of membrane fusion
9. Vaccines and the induction of functional antibodies: Time to look beyond the molecules of natural infection?
10. Three-dimensional solution structure of the 44 kDa ectodomain of SIV gp41
11. Monomer-trimer equilibrium of the ectodomain of SIV gp41: Insight into the mechanism of peptide inhibition of HIV infection
12. Effects of amino acid changes in the extracellular domain of the human immunodeficiency virus type 1 gp41 envelope glycoprotein
13. The human immunodeficiency virus type 1 gp120 V2 domain mediates gp41-independent intersubunit contacts
14. Oligomeric structure of virion-associated and soluble forms of the simian immunodeficiency virus envelope protein in the prefusion activated conformation
15. Core structure of gp41 from the HIV envelope glycoprotein
16. HIV entry and its inhibition
17. The leucine zipper motif of the envelope glycoprotein ectodomain of human immunodeficiency virus type 1 contains conformationally flexible regions as revealed by NMR and circular dichroism studies in different media
18. Expression, purification, and characterization of gp160e, the soluble, trimeric ectodomain of the simian immunodeficiency virus envelope glycoprotein, gp160
19. Functional role of the zipper motif region of human immunodeficiency virus type 1 transmembrane protein gp41
20. Mutations in the leucine zipper-like heptad repeat sequence of human immunodeficiency virus type 1 gp41 dominantly interfere with wild-type virus infectivity
21. Determination of the helix and beta form of proteins in aqueous solution by circular dichroism
22. + T-cell-depleted rhesus macaques
23. Beyond receptor expression: The influence of receptor conformation, density, and affinity in HIV-1 infection
24. HIV-1 membrane fusion: Targets of opportunity
25. Epitope map of human immunodeficiency virus type 1 gp41 derived from 47 monoclonal antibodies produced by immunization with oligomeric envelope protein
26. Native oligomeric human immunodeficiency virus type 1 envelope glycoprotein elicits diverse monoclonal antibody reactivities
27. Oligomeric structure of the human immunodeficiency virus type 1 envelope glycoprotein
28. Mutational analysis of the assembly domain of the HIV-1 envelope glycoprotein
29. Folding, interaction with GRP78-BiP, assembly, and transport of the human immunodeficiency virus type 1 envelope protein
30. Mechanisms of viral membrane fusion and its inhibition
31. Spectroscopic determination of tryptophan and tyrosine in proteins
32. Stabilization of human immunodeficiency virus type 1 envelope glycoprotein trimers by disulfide bonds introduced into the gp41 glycoprotein ectodomain
33. Interactions of polyclonal and monoclonal anti-glycoprotein 120 antibodies with oligomeric glycoprotein 120-glycoprotein 41 complexes of a primary HIV type 1 isolate: Relationship to neutralization
34. HIV-1 gp41 six-helix bundle formation occurs rapidly after the engagement of gp120 by CXCR4 in the HIV-1 Env-mediated fusion process
35. Recognition by human monoclonal antibodies of free and complexed peptides representing the prefusogenic and fusogenic forms of human immunodeficiency virus type 1 gp41
36. New insights into the spring-loaded conformational change of influenza virus hemagglutinin
37. Crystal structure of an isoleucine-zipper trimer
38. gp41, a multifunctional protein involved in HIV entry and pathogenesis
39. Thermodynamics of trimer-of-hairpins formation by the SIV gp41 envelope protein
40. Buried polar interactions and conformational stability in the simian immunodeficiency virus (SIV) gp41 core
41. A conformation-specific monoclonal antibody reacting with fusion-active gp41 from the human immunodeficiency virus type 1 envelope glycoprotein
42. Analysis of data from the analytical ultracentrifuge by nonlinear leastsquares techniques
43. Rapid and efficient site-specific mutagenesis without phenotypic selection
44. Structures of HIV-1 gp120 envelope glycoproteins from laboratory-adapted and primary isolates
45. Structure of an HIV gp120 envelope glycoprotein in a complex with the CD4 receptor and a neutralizing human antibody
46. A single amino acid change in the cytoplasmic domain of the simian immunodeficiency virus transmembrane molecule increases envelope glycoprotein expression on infected cells
47. Computer-aided interpretation of analytical sedimentation data for proteins
48. Structural and functional analysis of the HIV gp41 core containing an Ile573 to Thr substitution: Implications for membrane fusion
49. Mutations that destabilize the gp41core are determinants for stabilizing the Simian Immunodeficiency Virus-CPmac envelope glycoprotein complex
50. Subdomain folding and biological activity of the core structure from human immunodeficiency virus type 1 gp41: Implications for viral membrane fusion
51. Structural and functional analysis of interhelical interactions in the human immunodeficiency virus type 1 gp41 envelope glycoprotein by alanine-scanning mutagenesis
52. Immunogenicity of DNA vaccines expressing human immunodeficiency virus type 1 envelopeglycoprotein with and without deletions in the V1/2 and V3 regions
53. Crystal structure of the simian immunodeficiency virus (SIV) gp41 core: Conserved helical interactions underlie the broad inhibitory activity of gp41 peptides
54. Endoproteolytic cleavage of gp160 is required for the activation of human immunodeficiency virus
55. Evidence that the transition of HIV-1 gp41 into a six-helix bundle, not the bundle configuration, induces membrane fusion
56. HIV. Envelope's letters boxed into shape
57. HIV-1 neutralization: The consequences of viral adaptation to growth on transformed T cells
58. Genetic subtypes, humoral immunity, and human immunodeficiency virus type 1 vaccine development
59. Antibody cross-competition analysis of the human immunodeficiency virus type 1 gp120 exterior envelope glycoprotein
60. New targets for inhibitors of HIV-1 replication
61. Quintuple deglycosylation mutant of simian immunodeficiency virus SIVmac239 in rhesus macaques: Robust primary replication, tightly contained chronic infection, and elicitation of potent immunity against the parental wild-type strain
62. Maturation of HIV envelope glycoprotein precursors by cellular endoproteases
63. Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbons
64. Mapping of epitopes exposed on intact human immunodeficiency virus type 1 (HIV-1) virions: A new strategy for studying the immunologic relatedness of HIV-1
65. Host cell glycosylation of viral glycoproteins - A battlefield for host defence and viral resistance
66. The human immunodeficiency virus type 1 envelope glycoprotein precursor acquires aberrant intermolecular disulfide bonds that may prevent normal proteolytic processing
67. Fine definition of the epitope on the gp41 glycoprotein of human immunodeficiency virus type 1 for the neutralizing monoclonal antibody 2F5
68. HIV-1 antibody - Debris or virion?
69. Neutralization of human immunodeficiency virus type 1 by antibody to gp120 is determined primarily by occupancy of sites on the virion irrespective of epitope specificity
70. The neutralizing antibody response to HIV-1: Viral evasion and escape from humoral immunity
71. SIV gp41 binds to membranes both in the monomeric and trimeric states: Consequences for the neuropathology and inhibition of HIV infection
72. gp120: Biologic aspects of structural features
73. Human immunodeficiency virus type 1 envelope glycoprotein oligomerization requires the gp41 amphipathic alpha-helical/leucine zipperlike sequence
74. Specific single or double proline substitutions in the "springloaded" coiled-coil region of the influenza hemagglutinin impair or abolish membrane fusion activity
75. A role for carbohydrates in immune evasion in AIDS
76. A conserved HIV gp120 glycoprotein structure involved in chemokine receptor binding
77. Human monoclonal antibodies to the human immunodeficiency virus type 1 (HIV-1) transmembrane glycoprotein gp41 enhance HIV-1 infection in vitro
78. Variable-loop-deleted variants of the human immunodeficiency virus type 1 envelope glycoprotein can be stabilized by an intermolecular disulfide bond between the gp120 and gp41 subunits
79. The mannose-dependent epitope for neutralizing antibody 2G12 on human immunodeficiency virus type 1 glycoprotein gp120
80. Human immunodeficiency virus type I neutralization is determined by epitope exposure on the gp120 oligomer
81. Epitope exposure on functional, oligomeric HIV-1 gp41 molecules
82. Oligomeric and conformational properties of a proteolytically mature, disulfide-stabilized human immunodeficiency virus type 1 gp140 envelope glycoprotein
83. Trimerization specificity in HIV-1 gp41: Analysis with a GCN4 leucine zipper model
84. Biophysical characterization of recombinant proteins expressing the leucine zipper-like domain of the human immunodeficiency virus type 1 transmembrane protein gp41
85. Purification and characterization of oligomeric glycoprotein from a primary R5 subtype B human immunodeficiency virus for vaccine applications
86. Generation and structural analysis of soluble oligomeric gp140 envelope proteins derived from neutralization-resistant and neutralization-susceptible primary HIV type 1 isolates
87. A potent cross-clade neutralizing human monoclonal antibody against a novel epitope on gp41 of human immunodeficiency virus type 1
88. Atomic structure of a thermostable subdomain of HIV-1 gp41
89. Human monoclonal antibody 98-6 reacts with the fusogenic form of gp41
90. Characterization of conserved human immunodeficiency virus type 1 gp120 neutralization epitopes exposed upon gp120-CD4 binding
91. CD4-dependent, antibody-sensitive interactions between HIV-1 and its coreceptor CCR-5
92. Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1
93. Atomic structure of the ectodomain from HIV-1 gp41
94. Mutational analysis of residues in the coiled-coil domain of human immunodeficiency virus type 1 transmembrane protein gp41
95. Structure-function studies of the self-assembly domain of the human immunodeficiency virus type 1 transmembrane protein gp41
96. The antigenic structure of the HIV gp120 envelope glycoprotein
97. Involvement of the V1/V2 variable loop structure in the exposure of human immunodeficiency virus type 1 gp120 epitopes induced by receptor binding
98. The HIV-1 envelope glycoproteins: Fusogens, antigens, and immunogens
99. Epitope mapping of two immunodominant domains of gp41, the transmembrane protein of human immunodeficiency virus type 1, using ten human monoclonal antibodies
100. Characterization of stable, soluble trimers containing complete ectodomains of human immunodeficiency virus type 1 envelope glycoproteins
101. Modifications that stabilize human immunodeficiency virus envelope glycoprotein trimers in solution
102. Highly stable trimers formed by human immunodeficiency virus type 1 envelope glycoproteins fused with the trimeric motif of T4 bacteriophage fibritin
103. Improved elicitation of neutralizing antibodies against primary human immunodeficiency viruses by soluble stabilized envelope glycoprotein trimers
104. Expression, purification, and characterization of recombinant HIV gp140. The gp41 ectodomain of HIV or simian immunodeficiency virus is sufficient to maintain the retroviral envelope glycoprotein as a trimer
105. Broadly neutralizing antibodies targeted to the membraneproximal external region of human immunodeficiency virus type 1 glycoprotein gp41