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CC CKR5 cDNA was excised from plasmid p8.5 (22) [cDNA in pBluescript (Stratagene)] by Sma I-Xho I digestion (Sma I digestion eliminated most of the 5′ untranslated region). The Sma I-Xho I fragment was blunt-ended using the Klenow fragment of Escherichia coli DNA polymerase and ligated into the Stu I site of plasmid pSC59. The resulting plasmid, designated pGA9-CKR5, contains the CC CKR5 cDNA linked to a strong synthetic early-late vaccinia promoter (28).
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8944259046
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note
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G.A. was supported by the Dr. Nathan Davis Award from the American Medical Association Education and Research Foundation awarded to E.A.B.; C.C. and C.C.B. are recipients of NIH Intramural Research Training Awards; Y.F. is a recipient of a National Research Council Research Associate fellowship. Support was provided by the NIH Intramural AIDS Targeted Antiviral Program. We are grateful to P. Earl, NIAID, for helpful comments on the manuscript.
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