Stability of single copy transgene expression in CHOK1 cells is affected by histone modifications but not by DNA methylation

Journal of Biotechnology

Volumn 195, Issue 1, 2015, Pages 15-29

  Spencer, Shawal ^a   Gugliotta, Agustina ^a,d   Koenitzer, Jennifer ^b   Hauser, Hansjörg ^a   Wirth, Dagmar ^a,c  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b BOEHRINGER INGELHEIM PHARMA GMBH AND CO KG   (Germany)

^c HANNOVER MEDICAL SCHOOL   (Germany)

^d UNIVERSIDAD NACIONAL DEL LITORAL   (Argentina)

Author keywords

DNA methylation; Epigenetic stability; HDACi; Heterogeneity; Silencing of recombinant gene expression

Indexed keywords

ALKYLATION; CELLS; CHROMOSOMES; CLONE CELLS; CYTOLOGY; DNA; GENES; MAMMALS; METHYLATION;

CHROMOSOMAL INTEGRATION; DNA METHYLATION; HDACI; HETEROGENEITY; HETEROLOGOUS EXPRESSION; HISTONE DEACETYLASES; RECOMBINANT GENE EXPRESSIONS; TRANSGENE EXPRESSION;

GENE EXPRESSION;

HISTONE DEACETYLASE; CHROMATIN; HISTONE;

ARTICLE; CELL CLONE; CELL FRACTIONATION; CELL POPULATION; CELLS; CONTROLLED STUDY; DNA INTEGRATION; DNA METHYLATION; GENE EXPRESSION; GENE SILENCING; GENETIC HETEROGENEITY; GENETIC STABILITY; GENETIC TRANSFECTION; GENETIC VARIABILITY; HETEROLOGOUS EXPRESSION; HISTONE ACETYLATION; HISTONE MODIFICATION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; RANDOMIZED CONTROLLED TRIAL; SINGLE CELL ANALYSIS; TRANSGENE; ANIMAL; CHEMISTRY; CHO CELL LINE; CHROMATIN; CRICETULUS; CYTOMEGALOVIRUS; GENETICS; HAMSTER; HEK293 CELL LINE; HUMAN; METABOLISM;

MAMMALIA;

ANIMALS; CHO CELLS; CHROMATIN; CRICETINAE; CRICETULUS; CYTOMEGALOVIRUS; DNA METHYLATION; GENE SILENCING; HEK293 CELLS; HISTONES; HUMANS; PROMOTER REGIONS, GENETIC; TRANSGENES;

EID: 84937114189     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2014.12.009     Document Type: Article

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