Recombinant protein expression by targeting pre-selected chromosomal loci

BMC Biotechnology

Volumn 9, Issue , 2009, Pages

  Nehlsen, Kristina ^a   Schucht, Roland ^a   da Gama Norton, Leonor ^a,b   Krömer, Wolfgang ^b   Baer, Alexandra ^d   Cayli, Aziz ^c   Hauser, Hansjörg ^a   Wirth, Dagmar ^a  

^a HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^b UNIVERSIDADE NOVA DE LISBOA   (Portugal)

^c Cellca GmbH   (Germany)

^d Symphogen AS   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY EXPRESSION; ANTIBODY PRODUCTION; CELL CLONES; CELL LINES; CONTROL ELEMENTS; DESIGN VECTORS; EXPRESSION CONSTRUCTS; HEK293 CELLS; HIGH LEVEL EXPRESSION; INDIVIDUAL CELLS; MAMMALIAN CELLS; PLASMID TRANSFECTION; PRE-SELECTED; PROOF OF PRINCIPLES; PROTEIN EXPRESSIONS; RANDOM TRANSFER; RECOMBINANT PROTEIN EXPRESSION; REGULATORY ELEMENTS; REPORTER-GENE EXPRESSION; RETROVIRAL INFECTIONS; TARGET SITES; TRANS-GENES;

BIOACTIVITY; BIODEGRADATION; CELL CULTURE; CLONE CELLS; GENE EXPRESSION; MAMMALS; NUCLEIC ACIDS;

ANTIBODIES;

RECOMBINANT PROTEIN; RECOMBINASE; RETROVIRUS VECTOR;

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; BIOTECHNOLOGY; CELL CLONE; CELL STRAIN HEK293; CHO CELL; CHROMOSOME ANALYSIS; CONTROLLED STUDY; GENE CASSETTE; GENE EXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; ANIMAL; BIOSYNTHESIS; CRICETULUS; GENE TARGETING; GENE VECTOR; HAMSTER; METHODOLOGY; TRANSGENE;

MAMMALIA;

ANIMALS; ANTIBODY FORMATION; CHO CELLS; CLONE CELLS; CRICETINAE; CRICETULUS; GENE TARGETING; GENETIC VECTORS; HUMANS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; TRANSFECTION; TRANSGENES;

EID: 74749086258     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-9-100     Document Type: Article

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