Promoter methylation and transgene copy numbers predict unstable protein production in recombinant chinese hamster ovary cell lines

Biotechnology and Bioengineering

Volumn 108, Issue 11, 2011, Pages 2670-2681

  Osterlehner, Andrea ^a   Simmeth, Silke ^a   Göpfert, Ulrich ^a  

^a ROCHE DIAGNOSTICS GMBH   (Germany)

Author keywords

Cell line development; CHO cells; Gene copy number; Promoter methylation; Stability

Indexed keywords

BIOPROCESSES; CELL LINES; CHINESE HAMSTER OVARY CELLS; CHO CELL; CHO CELLS; DINUCLEOTIDES; GENE COPY NUMBER; HETEROLOGOUS PROMOTERS; HUMAN CYTOMEGALOVIRUS; LONG TERM STABILITY; MAMMALIAN CELL LINES; MULTIPLE CELLS; PRODUCT QUALITY; PROMOTER METHYLATION; PROTEIN PRODUCTION; RECOMBINANT PROTEIN PRODUCTIONS; SENSITIVE MEASUREMENT; TRANSGENE COPY NUMBERS;

ALKYLATION; CELL CULTURE; CONVERGENCE OF NUMERICAL METHODS; FLEXIBLE MANUFACTURING SYSTEMS; GENES; MAMMALS; METHYLATION; POLYMERASE CHAIN REACTION; PROTEINS; STABILITY;

CELLS;

RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; CHO CELL; CYTOMEGALOVIRUS; DNA METHYLATION; GENE DOSAGE; IMMEDIATE EARLY GENE; NONHUMAN; NUCLEOTIDE SEQUENCE; PREDICTION; PROMOTER REGION; PROTEIN SYNTHESIS; REACTION ANALYSIS; TRANSGENE;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; CYTOMEGALOVIRUS; DNA METHYLATION; GENE DOSAGE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENOMIC INSTABILITY; PROMOTER REGIONS, GENETIC; REAL-TIME POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; TRANSGENES;

CRICETULUS GRISEUS; HUMAN HERPESVIRUS 5; MAMMALIA;

EID: 80053053600     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23216     Document Type: Article

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