Effect of trichostatin A and 5-Aza-2′-deoxycytidine on transgene reactivation and epigenetic modification in transgenic pig fibroblast cells

Molecular and Cellular Biochemistry

Volumn 355, Issue 1-2, 2011, Pages 157-165

  Kong, Qingran ^a   Wu, Meiling ^a   Wang, Zhenkun ^b   Zhang, Xinmiao ^a   Li, Lu ^a   Liu, Xiangyu ^a   Mu, Yanshuang ^a   Liu, Zhonghua ^a  

^a NORTHEAST AGRICULTURAL UNIVERSITY   (China)

^b HARBIN MEDICAL UNIVERSITY   (China)

Author keywords

5 Aza dC; Acetylation; Methylation; Transgene expression; TSA

Indexed keywords

5 AZA 2' DEOXYCYTIDINE; GREEN FLUORESCENT PROTEIN; TRICHOSTATIN A;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL DEATH; CELL MEMBRANE; CELL NUCLEUS MEMBRANE; CELL STRUCTURE; CELL SWELLING; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOMEGALOVIRUS; DNA METHYLATION; DNA MODIFICATION; EPIGENETICS; FIBROBLAST; FIBROBLAST CULTURE; GENE EXPRESSION; HISTONE ACETYLATION; HISTONE MODIFICATION; IN VITRO STUDY; MOLECULAR INTERACTION; MORPHOLOGY; NONHUMAN; PORCINE FETAL FIBROBLAST; SILENCER GENE; TRANSGENE; TRANSGENIC PIG;

ACETYLATION; ANIMALS; ANIMALS, GENETICALLY MODIFIED; AZACITIDINE; CELL SHAPE; CELL SURVIVAL; CELLS, CULTURED; CYTOMEGALOVIRUS; DNA METHYLATION; EPIGENESIS, GENETIC; FIBROBLASTS; GENE EXPRESSION REGULATION; GREEN FLUORESCENT PROTEINS; HISTONE DEACETYLASE INHIBITORS; HISTONES; HYDROXAMIC ACIDS; METHYLTRANSFERASES; PROMOTER REGIONS, GENETIC; SWINE; TRANSGENES;

CYTOMEGALOVIRUS; SUIDAE; SUS;

EID: 80051544731     PISSN: 03008177     EISSN: 15734919     Source Type: Journal    
DOI: 10.1007/s11010-011-0849-7     Document Type: Article

References (32)

1. LM Houdebine 2000 Transgenic animal bioreactors Transgenic Res 9 305 320 11131009 10.1023/A:1008934912555 1:CAS:528:DC%2BD3cXovVajtLY%3D
2. AR Migliaccio C Bengra J Ling 2000 Stable and unstable transgene integration sites in the human genome: extinction of the green fluorescent protein transgene in K562 cells Gene 256 197 214 11054549 10.1016/S0378-1119(00) 00353-X 1:CAS:528:DC%2BD3cXosVGit78%3D
3. Y Yamauchi B Doe A Ajduk 2007 Genomic DNA damage in mouse transgenesis Biol Reprod 77 803 812 17652664 10.1095/biolreprod.107.063040 1:CAS:528:DC%2BD2sXht1CnurfK (Pubitemid 350014168)
4. WL Kaufman I Kocman V Agrawal, et al. 2008 Homogeneity and persistence of transgene expression by omitting antibiotic selection in cell line isolation Nucl Acids Res 36 e111 18682524 10.1093/nar/gkn508
5. CS Swindle CA Klug 2002 Mechanisms that regulate silencing of gene expression from retroviral vectors J Hematother Stem Cell Res 11 449 456 12183830 10.1089/15258160260090915 1:CAS:528:DC%2BD38Xlt1ejsr4%3D (Pubitemid 34615030)
6. TB Miranda PA Jones 2007 DNA methylation: the nuts and bolts of repression J Cell Physiol 213 384 390 17708532 10.1002/jcp.21224 1:CAS:528:DC%2BD2sXhtFKnu7rF (Pubitemid 47509717)
7. M Esteller JG Herman 2002 Cancer as an epigenetic disease: DNA methylation and chromatin alterations in human tumours J Pathol 196 1 7 11748635 10.1002/path.1024 1:CAS:528:DC%2BD38XhtFOhs7g%3D (Pubitemid 34071572)
8. T Kouzarides 2007 Chromatin modifications and their function Cell 128 693 705 17320507 10.1016/j.cell.2007.02.005 1:CAS:528:DC%2BD2sXis12ju7Y%3D (Pubitemid 46273577)
9. EM Duncan TL Muratore-Schroeder RG Cook 2008 Cathepsin L proteolytically processes histone H3 during mouse embryonic stem cell differentiation Cell 135 284 294 18957203 10.1016/j.cell.2008.09.055 1:CAS:528:DC%2BD1cXhtlWhsrzO
10. JS Lee A Shukla J Schneider 2007 Histone crosstalk between H2B monoubiquitination and H3 methylation mediated by COMPASS Cell 131 1084 1096 18083099 10.1016/j.cell.2007.09.046 1:CAS:528:DC%2BD1cXksFGgtw%3D%3D (Pubitemid 350235018)
11. F Li M Huarte M Zaratiegui 2008 Lid2 is required for coordinating H3K4 and H3K9 methylation of heterochromatin and euchromatin Cell 135 272 283 18957202 10.1016/j.cell.2008.08.036 1:CAS:528:DC%2BD1cXhtlWhsrzN
12. G Grassi 2003 Inhibitors of DNA methylation and histone deacetylation activate cytomegalovirus promoter-controlled reporter gene expression in human glioblastoma cell line U87 Carcinogenesis 24 1625 1635 12869421 10.1093/carcin/bgg118 1:CAS:528:DC%2BD3sXotVCls7Y%3D (Pubitemid 37304667)
13. KyungHyun Choi Hesham Basma J Singh 2005 Activation of CMV promoter-controlled glycosyltransferase and β-galactosidase glycogenes by butyrate, tricostatin A, and 5-Aza-2′-deoxycytidine J Glycoconjug 22 63 69 10.1007/s10719-005-0326-1 1:CAS:528:DC%2BD2MXjvVyiu7s%3D (Pubitemid 40674816)
14. N Cervoni M Szyf 2001 Demethylase activity is directed by histone acetylation J Biol Chem 276 40778 40787 11524416 10.1074/jbc.M103921200 1:CAS:528:DC%2BD3MXosVKhurg%3D
15. ZH Liu J Song ZK Wang JT Tian QR Kong Z Zheng 2008 Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer Chin Sci Bull 53 1035 1039 10.1007/s11434-008-0168-x 1:CAS:528:DC%2BD1cXmsValtrg%3D (Pubitemid 351738057)
16. C Grunau SJ Clark A Rosenthal 2001 Bisulfite genomic sequencing: systematic investigation of critical experimental parameters Nucl Acids Res 29 e65 11433041 10.1093/nar/29.13.e65 1:STN:280:DC%2BD3MzovF2qsw%3D%3D
17. T Jenuwein CD Allis 2001 Translating the histone code Science 293 1074 1080 11498575 10.1126/science.1063127 1:CAS:528:DC%2BD3MXmtVWltro%3D (Pubitemid 32758077)
18. G Robertson D Garrick M Wilson 1996 Age-dependent silencing of globin transgenes in the mouse Nucl Acids Res 24 1465 1471 8628679 10.1093/nar/24.8. 1465 1:CAS:528:DyaK28Xis1SmsLc%3D (Pubitemid 26118979)
19. G Villuendas A Gutierrez-Adan A Jimenez 2001 CMV-driven expression of green fluorescent protein (GFP) in male germ cells of transgenic mice and its effect on fertility Int J Androl 24 300 305 11554988 10.1046/j.1365-2605.2001. 00302.x 1:CAS:528:DC%2BD3MXnvVOlsb8%3D (Pubitemid 34225155)
20. AR Brooks RN Harkins P Wang 2004 Transcriptional silencing is associated with extensive methylation of the CMV promoter following adenoviral gene delivery to muscle J Gene Med 6 395 404 15079814 10.1002/jgm.516 1:CAS:528:DC%2BD2cXksVSnt70%3D (Pubitemid 40228056)
21. Q Kong M Wu Y Huan L Zhang H Liu Z Liu 2009 Transgene expression is associated with copy number and cytomegalovirus promoter methylation in transgenic pigs PLoS ONE 4 e6679 19688097 10.1371/journal.pone.0006679
22. EE Cameron KE Bachman S Myohanen 1999 Synergy of demethylation and histone deacetylase inhibition in the re-expression of genes silenced in cancer Nat Genet 21 103 107 9916800 10.1038/5047 1:CAS:528:DyaK1MXltlWjtA%3D%3D (Pubitemid 29036293)
23. KH Choi H Basma J Singh 2005 Activation of CMV promoter controlled glycosyltransferase and b-galactosidase glycogenes by butyrate, trichostatin A, and 5-Aza-29-deoxycytidine Glycoconjugate J 22 63 69 10.1007/s10719-005-0326-1 1:CAS:528:DC%2BD2MXjvVyiu7s%3D (Pubitemid 40674816)
24. J Singh GA Khan L Kinarsky 2004 Identification of disulfide bonds among the nine core 2 N-acetylglucosaminyltransferase-M cysteines conserved in the mucin β6N-acetylglucosaminyltransferase family J Biol Chem 279 38969 38977 15226299 10.1074/jbc.M401046200 1:CAS:528:DC%2BD2cXnt1yjsLk%3D (Pubitemid 39296058)
25. A Bartoli K Fettucciari I Fetriconi 2003 Effect of trichostatin a and 5-azacytidine on transgene reactivation in U937 transduced cells Pharmacol Res 48 111 118 12770523 1:CAS:528:DC%2BD3sXktVOgt7o%3D (Pubitemid 36593366)
26. BM Kumar HF Jin JG Kim 2006 DNA methylation levels in porcine fetal fibroblasts induced by an inhibitor of methylation, 5-azacytidine Cell Tissue Res 325 445 454 10.1007/s00441-006-0201-9 1:CAS:528:DC%2BD28Xnt1agt7o%3D (Pubitemid 44126159)
27. T Suganuma JL Workman 2008 Crosstalk among histone modifications Cell 135 604 607 19013272 10.1016/j.cell.2008.10.036 1:CAS:528:DC%2BD1cXhsVCls7rJ
28. V Mutskov G Felsenfeld 2004 Silencing of transgene transcription precedes methylation of promoter DNA and histone H3 lysine 9 EMBO 23 138 149 10.1038/sj.emboj.7600013 1:CAS:528:DC%2BD2cXhtVeqt7Y%3D (Pubitemid 38165754)
29. MJ Pikaart F Recillas-Targa G Felsenfeld 1998 Loss of transcriptional activity of a transgene is accompanied by DNA methylation and histone deacetylation and is prevented by insulators Genes Dev 12 2852 2862 9744862 10.1101/gad.12.18.2852 1:CAS:528:DyaK1cXmtFCku7o%3D (Pubitemid 28440144)
30. JA Oyer A Chu S Brar 2009 Aberrant epigenetic silencing is triggered by a transient reduction in gene expression PLoS ONE 4 e4832 19279688 10.1371/journal.pone.0004832
31. VJ Mutskov CM Farrell PA Wade 2002 The barrier function of an insulator couples high histone acetylation levels with specific protection of promoter DNA from methylation Genes Dev 16 1540 1554 12080092 10.1101/gad.988502 1:CAS:528:DC%2BD38XltVGmtLc%3D (Pubitemid 34686335)
32. W Reik 2007 Stability and flexibility of epigenetic gene regulation in mammalian development Nature 447 425 432 17522676 10.1038/nature05918 1:CAS:528:DC%2BD2sXlsFOgs7w%3D (Pubitemid 46816749)