Expression of difficult-to-remove host cell protein impurities during extended Chinese hamster ovary cell culture and their impact on continuous bioprocessing

Biotechnology and Bioengineering

Volumn 112, Issue 6, 2015, Pages 1232-1242

  Valente, Kristin N ^a   Lenhoff, Abraham M ^a   Lee, Kelvin H ^a  

^a UNIVERSITY OF DELAWARE   (United States)

Author keywords

Biotechnology; Chinese hamster ovary (CHO) cells; Continuous bioprocessing; Extended cell culture; Host cell proteins; Proteomics

Indexed keywords

ASSOCIATION REACTIONS; BIOTECHNOLOGY; CELL CULTURE; CELLS; CYTOLOGY; ELECTROPHORESIS; IMPURITIES; MOLECULAR BIOLOGY; PROTEINS; PURIFICATION; REMOVAL;

BIOPHARMACEUTICAL INDUSTRY; CHINESE HAMSTER OVARY CELLS; CONTINUOUS BIOPROCESSING; DOWNSTREAM PURIFICATIONS; HOST CELL PROTEIN; PROTEOMICS; RETENTION CHARACTERISTICS; TWO-DIMENSIONAL ELECTROPHORESIS (2DE);

CONTINUOUS CELL CULTURE;

BETA ACTIN; CATHEPSIN B; CATHEPSIN D; CLUSTERIN; COMPLEMENT COMPONENT C1R; COMPLEMENT COMPONENT C3; ENTACTIN; GLUCAN 1,4 ALPHA GLUCOSIDASE; GLUCOSE REGULATED PROTEIN; GLUTATHIONE TRANSFERASE; HOST CELL PROTEIN; LAMININ; LIPOPROTEIN LIPASE; METALLOPROTEINASE INHIBITOR; NERVE CELL ADHESION MOLECULE; NUCLEOSIDE DIPHOSPHATE KINASE A; NUCLEOSIDE DIPHOSPHATE KINASE B; PEROXIREDOXIN 1; PHOSPHOINOSITIDE DEPENDENT PROTEIN KINASE 1; PIGMENT EPITHELIUM DERIVED FACTOR; PROCOLLAGEN C PROTEINASE; PROSAPOSIN; PROTEIN; PROTEIN A; PROTEIN LYSINE 6 OXIDASE; PROTEOCHONDROITIN SULFATE; PROTEOCHONDROITIN SULFATE 4; SERINE PROTEINASE; SOMATOMEDIN BINDING PROTEIN 4; THROMBOSPONDIN 1; UNCLASSIFIED DRUG; BIOLOGICAL PRODUCT; PROTEOME; RECOMBINANT PROTEIN;

ANALYTIC METHOD; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL AGING; CELL COMPOSITION; CELL CULTURE; CELL GROWTH; CHO CELL LINE; CONTROLLED STUDY; DRUG INDUSTRY; EXTRACELLULAR MATRIX; GENE EXPRESSION; HOST CELL; NONHUMAN; PHASE SEPARATION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEOMICS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TWO DIMENSIONAL ELECTROPHORESIS; ANIMAL; BIOTECHNOLOGY; CRICETULUS; CULTURE TECHNIQUE; DRUG CONTAMINATION; FEMALE; ISOLATION AND PURIFICATION; METABOLISM; PHARMACEUTICS; PROCEDURES;

CRICETULUS GRISEUS;

ANIMALS; BIOLOGICAL PRODUCTS; BIOTECHNOLOGY; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETULUS; DRUG CONTAMINATION; FEMALE; GENE EXPRESSION; PROTEOME; PROTEOMICS; RECOMBINANT PROTEINS; TECHNOLOGY, PHARMACEUTICAL;

EID: 84928428004     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.25515     Document Type: Article

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