Antibody capture by mixed-mode chromatography: A comprehensive study from determination of optimal purification conditions to identification of contaminating host cell proteins

Journal of Chromatography A

Volumn 1218, Issue 45, 2011, Pages 8197-8208

  Pezzini, Jerome ^a,b   Joucla, Gilles ^a   Gantier, René ^b   Toueille, Magali ^b   Lomenech, Anne Marie ^c   Le Sénéchal, Caroline ^a   Garbay, Bertrand ^a   Santarelli, Xavier ^a   Cabanne, Charlotte ^a  

^a UNIVERSITÉ DE BORDEAUX   (France)

^b Pall Life Sciences   (France)

^c UNIVERSITÉ DE BORDEAUX   (France)

Author keywords

Antibody; Design of experiment; Host cell proteins; Mass spectrometry; Mixed mode chromatography; Screening

Indexed keywords

ANTIBODY CAPTURE; ANTIBODY PURIFICATION; CATIONIC GROUPS; COMPLEX BEHAVIOR; COMPREHENSIVE STUDIES; HOST CELL PROTEIN; LABORATORY COLUMNS; MICROPLATES; MIXED MODE; MIXED-MODE CHROMATOGRAPHY; OPTIMAL CONDITIONS; RECOMBINANT ANTIBODY; SUPERNATANTS;

CELL CULTURE; CHROMATOGRAPHY; DESIGN OF EXPERIMENTS; MASS SPECTROMETRY; OPTIMIZATION; PURIFICATION; RESINS;

ANTIBODIES;

ANTIBODY; CAPTO ADHERE RESIN; CATION; CELL PROTEIN; HEA HYPERCEL RESIN; MEP HYPERCEL RESIN; PPA HYPERCEL RESIN; RESIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBODY CAPTURE; ARTICLE; CHEMICAL PROCEDURES; CHO CELL; CHROMATOGRAPHY; COMPLEX FORMATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ELUTION; FEMALE; HOST CELL; HYDROPHOBICITY; MASS SPECTROMETRY; MIXED MODE CHROMATOGRAPHY; MOLECULAR DYNAMICS; NONHUMAN; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN PURIFICATION; SUPERNATANT;

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CHROMATOGRAPHY, LIQUID; CRICETINAE; CRICETULUS; ELECTRIC CONDUCTIVITY; HIGH-THROUGHPUT SCREENING ASSAYS; HYDROGEN-ION CONCENTRATION; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; LABORATORY CHEMICALS; MASS SPECTROMETRY; MODELS, CHEMICAL; PROTEINS; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS;

EID: 80054704670     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2011.09.036     Document Type: Article

References (38)

1. Shukla A.A., Thommes J. Trends Biotechnol. 2010, 28:253.
2. Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use: CBER, FDA, 94D-0259, 1997.
3. Chen J., Tetrault J., Ley A. J. Chromatogr. A 2008, 1177:272.
4. Follman D.K., Farner R.L. J. Chromatogr. A 2004, 1024(1-2):79.
5. Burton S.C., Harding D.R.K. J. Chromatogr. A 1998, 814:71.
6. Guerrier L., Flayeux I., Boschetti E. J. Chromatogr. B 2001, 755:37.
7. Boschetti E. Trends Biotechnol. 2002, 20:333.
8. Ghose S., Hubbard B., Cramer S.M. J. Chromatogr. A 2006, 1122:144.
9. Mowry M.C., Meagher M., Smith L., Marks J., Subramanian A. Protein Expr. Purif. 2004, 37:399.
10. Bak H., Thomas O.R.T. J. Chromatogr. B 2007, 848:116.
11. Chen J., Tetrault J., Zhang Y., Wasserman A., Conley G., DiLeo M., Haimes E., Nixon A.E., Ley A. J. Chromatogr. A 2010, 1217:216.
12. Weatherly G.T., Bouvier A., Lydiard D.D., Chapline J., Henderson I., Schrimsher J.L., Shepard S.R. J. Chromatogr. A 2002, 952:99.
13. Coulon D., Cabanne C., Fitton V., Noubhani A.M., Saint-Christophe E., Santarelli X. J. Chromatogr. B 2004, 808:111.
14. Dux M.P., Barent R., Sinha J., Gouthro M., Swanson T., Barthuli A., Inan M., Ross J.T., Smith L.A., Smith T.J., Webb R., Loveless B., Henderson I., Meagher M.M. Protein Expr. Purif. 2006, 45:359.
15. Rege K., Pepsin M., Falcon B., Steele L., Heng M. Biotechnol. Bioeng. 2006, 93:618.
16. Cabanne C., Pezzini J., Joucla G., Hocquellet A., Barbot C., Garbay B., Santarelli X. J. Chromatogr. A 2009, 1216:4451.
17. Queiroz J.A., Tomaz C.T., Cabral J.M. J. Biotechnol. 2001, 87(2):143.
18. Toueille M., Uzel A., Depoisier J.-F., Gantier R. J. Chromatogr. B 2011, 836.
19. Alizadeh-Pasdar N., Li-Chan E.C.Y. J. Agric. Food Chem. 2000, 48:328.
20. Xia F., Nagrath D., Cramer S.M. J. Chromatogr. A 2005, 1079:229.
21. Jennissen H.P. Hydrophobic Interaction Chromatography 2002, vol. 9. Nature Encyclopedia of Life Sciences (eLS), p. 353.
22. Hofstee B.H.J., Otillio N.F. J. Chromatogr. 1978, 159:57.
23. Makhatadze G.I., Privalov P.L. Biophys. Chem. 1994, 50:285.
24. Bywater R.P., Marsden N.V.B. J. Chromatogr. Libr. 1983, 22A:309.
25. Zhao G., Dong X.-Y., Sun Y. J. Biotechnol. 2009, 144:3.
26. Jin M., Szapiel N., Zhang J., Hickey J., Ghose S. Biotechnol. Bioeng. 2010, 105(2):306.
27. Shin Y.-K., Yoo B.C., Hong Y.S., Chang H.J., Jung K.H., Jeong S.-Y., Park J.-G. Electrophoresis 2009, 30:2182.
28. Wurm F.M. Nat. Biotechnol. 2004, 22(11):1393.
29. Alban J.R., Carpentier M., Lansiaux C. J. Fluoresc. 2008, 18:75.
30. Piez K.A., Trus B.L. J. Mol. Biol. 1977, 110(4):701.
31. Gaucher E.A., Das U.K., Miyamoto M.M., Benner S.A. Mol. Biol. Evol. 2002, 19(4):569.
32. Sieron A.L., Tretiakova A., Jameson B.A., Segall M.L., Lund-Katz S., Tariq Khan M., Li S.-W., Stocker W. Biochemistry 2000, 39:3231.
33. Saint-Jean M., Izard T., Sygusch J. J. Biol. Chem. 2007, 282(19):14309.
34. Bowman G.D., Nodelman I.M., Hong Y., Chua N.-H., Lindberg U., Schutt C.E. Proteins: Struct. Funct. Genet. 2000, 41:374.
35. Mark P., Nilsson L. Eur. Biophys. J. 2007, 36:213.
36. Gilmore W.S., Olwill S., Mc Glynn H., Alexander H.D. Biochem. Soc. Trans. 2004, 32:1122.
37. Ikeda Y., Noguchi T. J. Biol. Chem. 1998, 273:12227.
38. Bozzi M., Di Stasio E., O Cicero D., Giardina B., Paci M., Brancaccio A. Protein Sci. 2004, 13:2437.