Host cell protein dynamics in the supernatant of a mAb producing CHO cell line

Biotechnology and Bioengineering

Volumn 109, Issue 4, 2012, Pages 971-982

  Tait, A S ^a   Hogwood, C E M ^b   Smales, C M ^b   Bracewell, D G ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF KENT   (United Kingdom)

Author keywords

Chinese hamster ovary cells; Downstream processing; Host cell protein; Mammalian cell culture; Monoclonal antibody; Proteomics

Indexed keywords

CHINESE HAMSTER OVARY CELLS; DOWNSTREAM-PROCESSING; HOST CELL PROTEIN; MAMMALIAN CELL CULTURE; PROTEOMICS;

ANIMAL CELL CULTURE; CELLS; CYTOLOGY; MOLECULAR BIOLOGY; MONOCLONAL ANTIBODIES; PRINCIPAL COMPONENT ANALYSIS; PROCESS MONITORING;

PROTEINS;

CALRETICULIN; CELL PROTEIN; ELONGATION FACTOR 2; HOST CELL PROTEIN; MONOCLONAL ANTIBODY; PROTEIN DISULFIDE ISOMERASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBODY PRODUCING CELL; ARTICLE; CELL CULTURE; CELL GROWTH; CELL LINE; CELL VIABILITY; CHO CELL; CONTROLLED STUDY; CYTOLYSIS; DRUG MANUFACTURE; FED BATCH CULTURE; FEMALE; HOST CELL; LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY; MASS SPECTROMETRY; MOLECULAR DYNAMICS; NONHUMAN; PRINCIPAL COMPONENT ANALYSIS; PROCESS MONITORING; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECRETION; SUPERNATANT; SURFACE ENHANCED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; TANDEM MASS SPECTROMETRY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANIMALS; ANTIBODIES, MONOCLONAL; BATCH CELL CULTURE TECHNIQUES; BIOLOGICAL MARKERS; BIOREACTORS; CELL SURVIVAL; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; CRICETULUS; CULTURE MEDIA, CONDITIONED; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; GLUTAMATE-AMMONIA LIGASE; IMMUNOGLOBULIN G; ISOELECTRIC FOCUSING; PARTICLE SIZE; PROTEINS; PROTEOMICS; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TIME FACTORS;

CRICETULUS GRISEUS; MAMMALIA;

EID: 84857442070     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24383     Document Type: Article

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