Optimization of protein sample preparation for two-dimensional electrophoresis

Electrophoresis

Volumn 33, Issue 13, 2012, Pages 1947-1957

  Valente, Kristin N ^a   Choe, Leila H ^a   Lenhoff, Abraham M ^a   Lee, Kelvin H ^a  

^a UNIVERSITY OF DELAWARE   (United States)

Author keywords

Design of experiments; Proteomics; Sample preparation; Two dimensional electrophoresis

Indexed keywords

CELL CULTURE; DESIGN OF EXPERIMENTS; ELECTROPHORESIS; ESCHERICHIA COLI; METABOLISM; MOLECULAR BIOLOGY; PROTEINS; RECOVERY; SOLUBILITY; SUSPENSIONS (FLUIDS); THROUGHPUT;

CELL LYSATES; OPTIMISATIONS; PHYSICOCHEMICAL PROPERTY; PROTEIN RECOVERY; PROTEIN SAMPLES; PROTEIN SOLUBILITY; PROTEOMICS; SAMPLE PREPARATION; TOTAL PROTEIN; TWO DIMENSIONAL ELECTROPHORESIS;

UREA;

UREA;

ANIMAL CELL; ARTICLE; CELL LYSATE; CHO CELL; CONTROLLED STUDY; ESCHERICHIA COLI; EXPERIMENTAL DESIGN; FACTORIAL ANALYSIS; IMAGE ANALYSIS; METHODOLOGY; NONHUMAN; PHYSICAL CHEMISTRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEOMICS; TWO DIMENSIONAL ELECTROPHORESIS;

EID: 84864018362     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.201100659     Document Type: Article

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